DNA(RNA) Synthesis

DNA(RNA) Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.

In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.

M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.

DNA(RNA) Synthesis related products

Structure Cat No. Product Name CAS No. Product Description
V52845 (+)-TK216 1903783-77-6 (+)-TK216 is the enantiomer of TK216 (HY-122903).
V52847 (-)-TK216 1903783-78-7 (-)-TK216 is the enantiomer of TK216 (HY-122903).
V55140 (E/Z)-NSAH 54009-54-0 (E/Z)-NSAH is an isomer of NSAH (HY-114503).
V52527 1,4-Anthraquinone 635-12-1 1,4-Anthraquinone is a novel anticancer agent.
V53307 1-Hydroxyanthraquinone 129-43-1 1-Hydroxyanthraquinone is an orally active natural product derived from some plants such as Tabebuia avellanedae and has carcinogenic effects.
V77531 1-p-Chlorobenzyl-1,2,3-triazole-5′-O-DMT-dU Phosphoramidite
V77574 2'-Deoxy-2'-fluoroadenosine 5'-triphosphate tetralithium (AfTP (tetralithium), 2'-Fluoro-2'-deoxy-ATP (tetralithium))
V55148 2'-Deoxy-5'-O-DMT-N4-Fmoc-5-methylcytidine 3'-CE-phosphoramidite 176755-87-6 2'-Deoxy-5'-O-DMT-N4-Fmoc-5-methylcytidine 3'-CE-phosphoramidite is an analogue of the nucleoside cytosine.
V55158 2'-Deoxy-5'-O-DMT-N6-Fmoc-adenosine 3'CE-phosphoramidite 109420-86-2 2'-Deoxy-5'-O-DMT-N6-Fmoc-adenosine 3'CE-phosphoramidite is an analog of an adenine nucleoside.
V55184 2'-Deoxyguanosine-(N-iBu)-3'-methyl-phosphoramidite 84416-84-2 2'-Deoxyguanosine-(N-iBu)-3'-methyl-phosphoramidite is a phosphoramidite monomer that finds application in the synthesis of oligonucleotides.
V52985 2′-O-(2-Methoxyethyl)adenosine 168427-74-5 2′-O-(2-Methoxyethyl)adenosine is a compound useful in the synthesis of oligonucleotides.
V55168 2'-O-MOE-5MeU-3'-phosphoramidite (2'-O-MOE-5-ME-U phosphoramidite monomer) 163878-63-5 2'-O-MOE-5MeU-3'-phosphoramidite is a phosphoramidite monomer that finds application in the synthesis of oligonucleotides.
V53025 2'-OMe-Ac-C Phosphoramidite 199593-09-4 2'-OMe-Ac-C Phosphoramidite is a modified phosphoramidite that can be employed to synthesize oligonucleotides.
V55164 2'-OMe-dmf-G-CE-Phosphoramidite 128219-77-2 2'-OMe-dmf-G-CE-Phosphoramidite is a phosphoramidite monomer that finds application in the synthesis of oligonucleotides.
V56546 2,4-D-13C6 (2,4-Dichlorophenoxyacetic acid-13C6) 150907-52-1 2,4-D-13C6 is a 13C-labeled version of 2,4-D. 2,4-D (2,4-Dichlorophenoxyacetic acid) is a novel and seletive herbicide used for broadleaf weed control.
V56556 2,4-D-d3 202480-67-9 2,4-D-d3 is the deuterated form of 2,4-D. 2,4-D (2,4-Dichlorophenoxyacetic acid) is a selective herbicide used for broadleaf weed control.
V56560 2,4-D-d5 352438-69-8 2,4-D-d5 is the deuterated form of 2,4-D. 2,4-D (2,4-Dichlorophenoxyacetic acid) is a selective herbicide used for broadleaf weed control.
V52510 2-Aminofluorene 153-78-6 2-Aminofluorene is a synthetic chemical pesticide.
V56559 2-Aminofluorene-13C (2-Aminofluorene-13C) 335081-08-8 2-Aminofluorene-13C is 13C labeled 2-Aminofluorene.
V56549 2-Aminofluorene-d11 (2-Aminofluorene-d11) 347841-44-5 2-Aminofluorene-d11 is the deuterated product of 2-Aminofluorene.
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