- Calculate the Mass of a compound required to prepare a solution of known volume and concentration
- Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
- Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume

An example of molarity calculation using the molarity calculator is shown below:

What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

- Enter 350.26 in the Molecular Weight (MW) box
- Enter 10 in the Concentration box and choose the correct unit (mM)
- Enter 5 in the Volume box and choose the correct unit (mL)
- Click the “Calculate” button
- The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?

Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

- Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
- Enter 25 into the Concentration (End) box and select the correct unit (mM)
- Enter 25 into the Volume (End) box and choose the correct unit (mL)
- Click the “Calculate” button
- The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Instructions to calculate molar mass (molecular weight) of a chemical compound:

- To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

- Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
- Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

- Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
- Click the “Calculate” button
- The answer appears in the Volume (to add to vial) box

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

Calculation results

**Working concentration**：
mg/mL；

**Method for preparing DMSO stock solution**：
mg
drug pre-dissolved in
μL DMSO (stock solution concentration
mg/mL).* Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.*

**Method for preparing in vivo formulation:**：Take
μL
DMSO stock solution, next add
μL PEG300, mix and clarify, next addμL
Tween 80, mix and clarify, next add
μL ddH_{2}O，mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.

(2) Be sure to add the solvent(s) in order.