RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.
In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.
M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.
| Structure | Cat No. | Product Name | CAS No. | Product Description |
|---|---|---|---|---|
|
V55123 | 2-O-DMT-Sulfonyldiethanol phosphoramidite (phosphorylated monomer) | 108783-02-4 | 2-O-DMT-Sulfonyldiethanol phosphoramidite is a phosphoramidite monomer that may be utilized in the solid-phase synthesis of oligonucleotides. |
|
V52516 | 23-Oxa-OSW-1 (SBF-1) | 790224-37-2 | 23-Oxa-OSW-1, an analogue of OSW 1, is a potent sterol-binding protein (OSBP) inhibitor. |
|
V55161 | 3′-Deoxy-3′-methyluridine | 444020-64-8 | 3′-Deoxy-3′-methyluridine is a nucleoside analogue involved in the preparation of RNA-dependent RNA viral polymerase inhibitors. |
|
V55193 | 3'-Deoxy-5'-O-(4,4'-dimethoxytrityl)-3'-fluoro uridine-2'-CED-phosphoramidite | 129015-63-0 | 3'-Deoxy-5'-O-(4,4'-dimethoxytrityl)-3'-fluoro uridine-2'-CED-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55201 | 3'-DMTr-dA | 140712-82-9 | 3'-DMTr-dA is used for nucleotide synthesis. |
|
V55178 | 3'-F-3'-dA(Bz)-2'-Phosphoramidite | 2127174-09-6 | 3'-F-3'-dA(Bz)-2'-Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55125 | 3'-TBDMS-Bz-rA Phosphoramidite (3'-TBDMS-Bz-rA Phosphoramidite) | 129451-75-8 | 3'-TBDMS-Bz-rA Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55190 | 3'-TBDMS-ibu-rG Phosphoramidite | 1445905-51-0 | 3'-TBDMS-ibu-rG Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V77323 | 3'Ome-m7GpppAmpG ammonium | 3'Ome-m7GpppAmpG ammonium is a trinucleotide cap analog containing locked nucleic acid (LNA) molecules. | |
|
V55152 | 3-Cyanovinylcarbazole phosphoramidite | 1157899-72-3 | 3-Cyanovinylcarbazole phosphoramidite is a potent antiviral molecule that has the ability to stop the synthesis of viral DNA. |
|
V53337 | 3-Hydroxy-2-methylpyridine | 1121-25-1 | 3-Hydroxy-2-methylpyridine is extracted from theobromine extract and may be utilized in the preparation /synthesis of pyrimidines. |
|
V55180 | 4-Methoxyestradiol | 26788-23-8 | 4-Methoxyestradiol is methoxyestradiol that causes human lung epithelial cells to experience oxidative DNA damage. |
|
V53010 | 4-Methoxyphenethyl alcohol (p-Methoxyphenylethanol) | 702-23-8 | 4-Methoxyphenethyl alcohol, an aromatic alcohol, is the main component of the anise-like odor produced by Amorphophallus. |
|
V55177 | 5'-Cholesteryl-TEG phosphoramidite | 1325147-19-0 | 5'-Cholesteryl-TEG phosphoramidite is a phosphoramidite monomer that may be utilized in the solid-phase synthesis of oligonucleotides. |
|
V55181 | 5'-DMTr-dA(Bz)-Methyl phosphonamidite | 114079-05-9 | 5'-DMTr-dA(Bz)-Methyl phosphonamidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55187 | 5′-DMTr-dC (Ac)-Methylphosphonamidite | 178094-78-5 | 5'-DMTr-dC (Ac)-Methylphosphonamidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55195 | 5'-DMTr-dG(iBu)-Methyl phosphonamidite | 115131-08-3 | 5'-DMTr-dG(iBu)-Methyl phosphonamidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V55166 | 5'-DMTr-T-Methyl phosphonamidite | 114079-04-8 | 5'-DMTr-T-Methyl phosphonamidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
|
V53037 | 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine (5'-O-DMT-dU) | 23669-79-6 | 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine (5'-O-DMT-dU) is a novel inhibitor of Escherichia coli dUTP nucleotidyl hydrolase (dUTPase), Ki value Above 1000 μM. 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine synthesizes nucleoside phosphoramidite blocks, which are used in machine-assisted DNA synthesis. |
|
V55170 | 5'-O-DMT-5-Ethynyl-2'-deoxyuridine 3'-CE phosphoramidite | 188411-06-5 | 5'-O-DMT-5-Ethynyl-2'-deoxyuridine 3'-CE phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. |
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