| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
As a labeled sugar, D-Fructose-13C6 targets carbohydrate metabolic pathways. The isotopic labeling allows researchers to trace the metabolic pathways of fructose accurately, providing deeper insights into its conversion into other sugars and its role in energy production and storage. It is used as a metabolic tracer.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
D-Fructose-13C6 is used as a metabolic tracer in cell culture and biochemical assays. The 13C labeling allows for precise tracking of fructose metabolism and its conversion to other sugars, glycolytic intermediates, and energy metabolites. It is used to study fructose uptake, glycolysis, gluconeogenesis, and the pentose phosphate pathway in various cell types. |
| ln Vivo |
In vivo, D-Fructose-13C6 is used in metabolic tracing studies to investigate fructose metabolism in animal models. The isotopic labeling allows researchers to trace the metabolic fate of fructose, its conversion into other sugars, and its role in energy production and storage. It is used to study diabetes, metabolic syndrome, and other metabolic disorders.
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| Enzyme Assay |
D-Fructose-13C6 is used as a tracer in metabolic flux analysis. Cells or tissues are incubated with the labeled compound, and 13C enrichment in metabolic intermediates is analyzed by LC-MS/MS or NMR. For enzyme assays, the compound is used as a substrate for fructokinase or other fructose-metabolizing enzymes, and the labeled products are quantified by mass spectrometry.
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| Cell Assay |
For in vitro cell assays, cells are cultured in glucose-free medium supplemented with D-Fructose-13C6 at concentrations of 1-25 mM. Fructose uptake is measured by LC-MS/MS. Glycolytic flux is assessed by measuring lactate production and 13C incorporation into glycolytic intermediates. Metabolic flux analysis is performed using 13C tracing and mass spectrometry to follow the conversion of fructose into downstream metabolites.
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| Animal Protocol |
For in vivo animal studies, D-Fructose-13C6 is administered to rodents via oral gavage or intravenous injection at doses of 1-5 g/kg. Blood samples are collected at various time points for metabolic tracing. Plasma fructose and glucose levels are measured, and 13C enrichment in blood glucose, lactate, and other metabolites is quantified by LC-MS/MS or GC-MS. Metabolic flux analysis traces 13C incorporation into glycolytic and TCA cycle intermediates.
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| ADME/Pharmacokinetics |
D-Fructose-13C6 has a molecular weight of 186.11 g/mol. It is highly soluble in water. The compound is stable under standard storage conditions. The uniform 13C labeling provides a mass shift of +6 Da for MS detection. As fructose, it is rapidly absorbed and metabolized through the fructose and glycolytic pathways. It is a natural monosaccharide found in many plants.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available. Fructose is generally recognized as safe at moderate doses. The 13C-labeled form is not expected to exhibit additional toxicity. High doses may cause metabolic effects. Standard safety precautions should be followed.
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| References | |
| Additional Infomation |
D-Fructose-13C6 (CAS# 201595-65-5) is a stable isotope-labeled compound used primarily as a metabolic tracer in studies of carbohydrate metabolism, diabetes, and metabolic syndrome. It has not been investigated in clinical trials nor approved as a therapeutic drug. The compound is widely used in metabolic flux analysis and stable isotope tracing studies to investigate fructose metabolism and its role in health and disease.
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| Molecular Formula |
13C6H12O6
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|---|---|
| Molecular Weight |
186.11
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| Exact Mass |
186.084
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| CAS # |
201595-65-5
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| Related CAS # |
D-Fructose;57-48-7;D-Fructose-13C;108311-21-3;D-Fructose-13C-1;117013-19-1;D-Fructose-13C2;2483736-14-5;D-Fructose-6-13C;287100-63-4;D-Fructose-d7;D-Fructose-d-1;D-Fructose-13C6,d7;D-Fructose-d-2;80599-66-2;D-Fructose-d2;285979-75-1;D-Fructose-d2-1;478518-48-8;D(-)-Fructose-18O-1;D-Fructose-18O-2;D-Fructose-13C3;D-Fructose-13C3-1;D-Fructose-13C4
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| PubChem CID |
71310259
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| Appearance |
White to off-white solid powder
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| Density |
1.758g/cm3
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| Melting Point |
119-122ºC (dec.)(lit.)
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| Index of Refraction |
1.645
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| LogP |
-3.2
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
12
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| Complexity |
147
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| Defined Atom Stereocenter Count |
3
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| SMILES |
[13CH2]([13C@H]([13C@H]([13C@@H]([13C](=O)[13CH2]O)O)O)O)O
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| InChi Key |
BJHIKXHVCXFQLS-HJLYHLTLSA-N
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| InChi Code |
InChI=1S/C6H12O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3,5-9,11-12H,1-2H2/t3-,5-,6-/m1/s1/i1+1,2+1,3+1,4+1,5+1,6+1
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| Chemical Name |
(3S,4R,5R)-1,3,4,5,6-pentahydroxy(1,2,3,4,5,6-13C6)hexan-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (1343.29 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3732 mL | 26.8658 mL | 53.7317 mL | |
| 5 mM | 1.0746 mL | 5.3732 mL | 10.7463 mL | |
| 10 mM | 0.5373 mL | 2.6866 mL | 5.3732 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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