| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mL |
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| 100mL |
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| Other Sizes |
Purity: ≥98%
Tween 80 (Polysorbate 80; Montanox 80; Alkest TW 80; Tween 80; PS 80) is a surfactant, emulsifier, excipient and non-ionic detergent (for cell lysis), often used in foods and cosmetics. It is a synthetic compound and a viscous, water-soluble yellow liquid that can also reduce bacterial attachment and inhibit biofilm formation.
| ln Vitro |
Tween 80 (Polysorbate 80) is a surfactant that prevents the formation of biofilms and decreases cell division [1]. Solubility rose 100-fold in vitro when 1% Tween 80 was applied [2].
- Promoting bacterial cell dispersion: 0.05% Tween 80 can promote the dispersion of Salmonella enterica serovar Thompson cells in vitro. Digital image analysis of micrographs shows that in the leaf washes containing Tween 80, single - cell proportion in cell assemblages increases from 9.3% to 13.7%, and the percentage of the total cell population in aggregates equal to or larger than 64 cells decreases from 9.8% to 4.4%. It promotes the dispersal of cells from large aggregates into smaller ones and single cells [1] - Antagonizing smooth muscle contraction: Tween 80 has no direct stimulant or relaxant effect on guinea pig ileum or rat uterus in vitro, but it can antagonize the contractions induced by acetylcholine, histamine, barium, 5 - hydroxytryptamine and carbachol in a dose - dependent manner. It also has a direct relaxant effect on rabbit jejunum [4] - Myocardial depressant effect: On the electrically - driven guinea pig left atrial preparation, Tween 80 exhibits a dose - dependent negative inotropic action [4] |
|---|---|
| ln Vivo |
Tween 80 (Polysorbate 80) (IP; 10 mL/kg) lowers body weight by a large amount to 24–30 g[3].
- Central nervous system depression: Tween 80 can produce mild to moderate depression of the central nervous system in mice and rats, with a marked reduction in locomotor activity and rectal temperature, and can also exhibit ataxia and paralytic activity [4] - Hypotensive effect: Intravenous administration of Tween 80 in dogs has a dose - dependent hypotensive effect [4] |
| Animal Protocol |
- Mouse and rat experiments: In mice and rats, Tween 80 is administered intraperitoneally or orally to study its effect on the central nervous system, observing changes in locomotor activity, rectal temperature, ataxia and paralytic activity. The dosage should not exceed 1 ml/kg when used as a vehicle for neuropsychopharmacological experiments [4]
- Dog experiment: Intravenous administration of Tween 80 to dogs, and then observing its blood pressure changes to study its hypotensive effect [4] |
| Toxicity/Toxicokinetics |
Interactions
This study investigated the effects of oral administration of polysorbate 80 on the bioavailability and distribution of methotrexate (MTX) in NMRI and Porton mice. Results showed that this nonionic surfactant increased MTX concentrations in different organs of both mouse species. Tween 80 Enhanced the Antitumor Activity of Daunorubicin in Ehrlich ascites tumor rats and isolated tumor cells. At the subcellular level, Tween 80 enhanced the binding of daunorubicin to the cell nucleus and mitochondria. Treatment with the nonionic surfactant Tween 80 in Chinese hamster cell lines resistant to actinomycin D and daunorubicin enhanced the effects of these two antibiotics in growth studies and increased their uptake. Intravenous injection of an aqueous solution of doxorubicin containing 10% Tween 80 into tumor-bearing mice resulted in higher antitumor activity and increased doxorubicin levels in the spleen, lungs, and kidneys compared to doxorubicin alone. For more complete data on interactions of polysorbate 80 (7 in total), please visit the HSDB record page. Non-human toxicity values Rat intraperitoneal LD50: 6804 mg/kg Rat intravenous LD50: 1790 mg/kg Mouse oral LD50: 25000 mg/kg Mouse intraperitoneal LD50: 7600 mg/kg For more complete data on non-human toxicity of polysorbate 80 (6 in total), please visit the HSDB record page. Tween 80 has low toxicity in mice and rats when administered intraperitoneally and orally [4] |
| References | |
| Additional Infomation |
Tween 80 is a surfactant widely used as a solvent in pharmacological experiments. However, it is not suitable for in vitro tissue experiments or intravenous administration. It can be safely used as a solvent in neuropsychopharmacology experiments at doses not exceeding 1 ml/kg [4]
|
| Molecular Formula |
C32H60O10
|
|---|---|
| Molecular Weight |
604.8128
|
| Exact Mass |
604.418
|
| Elemental Analysis |
C, 63.55; H, 10.00; O, 26.45
|
| CAS # |
9005-65-6
|
| Related CAS # |
9005-65-6
|
| PubChem CID |
6364656
|
| Appearance |
Colorless to light yellow liquid
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
695.8±55.0 °C at 760 mmHg
|
| Melting Point |
-21ºC
|
| Flash Point |
207.1±25.0 °C
|
| Vapour Pressure |
0.0±5.0 mmHg at 25°C
|
| Index of Refraction |
1.502
|
| LogP |
4.8
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
31
|
| Heavy Atom Count |
42
|
| Complexity |
632
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCCCCCCC/C=C\CCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
|
| InChi Key |
HDTIFOGXOGLRCB-KTKRTIGZSA-N
|
| InChi Code |
InChI=1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-26-28(38-21-18-33)32-31(41-23-20-35)29(27-42-32)39-22-19-34/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9-
|
| Chemical Name |
2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl (Z)-octadec-9-enoate
|
| Synonyms |
Polysorbate 80; Montanox 80; sorbate80; DSSTox_CID_1175; DSSTox_RID_75992; DSSTox_GSID_21175; ...; CAS-9005-65-6; Alkest; TW 80; Tween 80; PS 80
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (1.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (1.91 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (1.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6534 mL | 8.2671 mL | 16.5341 mL | |
| 5 mM | 0.3307 mL | 1.6534 mL | 3.3068 mL | |
| 10 mM | 0.1653 mL | 0.8267 mL | 1.6534 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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