| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
GnRH Receptor (gonadotropin-releasing hormone receptor). sGnRH-A acetate is a GnRH analogue that binds to and activates the GnRH receptor on pituitary gonadotropes, stimulating the synthesis and secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It also stimulates growth hormone (GH) secretion via interaction with pituitary somatotropes.
|
|---|---|
| ln Vitro |
sGnRH-A acetate (0.1 nM-1 μM, 24 h) enhances GH secretion and raises GH mRNA levels in a dose-dependent manner [1]. Growth hormone levels are increased by about five times over the course of six hours when exposed to 10 nM sGnRH-A acetate [1].
In vitro, sGnRH-A acetate (0.1 nM-1 uM, 24 hours) increases GH mRNA levels and stimulates GH secretion in a dose-dependent manner. At 10 nM for 6 hours, it induces growth hormone levels nearly 5 times higher than the control in common carp pituitary fragments. The compound is a potent and efficacious GnRH receptor agonist in fish and mammalian systems. |
| ln Vivo |
In vivo, sGnRH-A acetate is used as an ovulation inducer for artificial insemination in various species including fish and rabbits. It stimulates growth hormone secretion and can be used to synchronize ovulation for breeding programs. In rabbits, sGnRH-A has been demonstrated as a potent ovulation inducer. The compound is widely used in aquaculture and reproductive research.
|
| Enzyme Assay |
GnRH receptor binding assay (non-cell-based): Membranes prepared from cells expressing GnRH receptors are incubated with 125I-labeled sGnRH-A (0.1 nM) and increasing concentrations of unlabeled sGnRH-A acetate (0.001-1000 nM) in binding buffer (25 mM HEPES pH 7.4, 1 mM CaCl2, 1 mM MgCl2, 0.5% BSA) for 90 min at 25degC. Bound radioactivity is separated by filtration through glass fiber filters and measured with a gamma counter. IC50/EC50 values are calculated by non-linear regression (n=3).
|
| Cell Assay |
Growth hormone secretion assay (cell-based): Pituitary fragments or primary pituitary cells are isolated from common carp or other species. Cells are seeded in 24-well plates and treated with sGnRH-A acetate (0-1000 nM) for 6-24 hours. Culture medium is collected, and GH levels are measured by ELISA or radioimmunoassay. GH mRNA levels are assessed by qRT-PCR from cell lysates. EC50 for GH stimulation is calculated by non-linear regression from triplicate wells.
|
| Animal Protocol |
Ovulation induction protocol (animal): Female rabbits (or other target species) are treated with sGnRH-A acetate via intramuscular injection or intravenous administration at doses of 0.5-20 ug/kg. For artificial insemination, sGnRH-A is administered at the time of insemination to induce ovulation. Ovulation is confirmed by laparoscopy or by recovery of oocytes/embryos from the reproductive tract. Timing of ovulation is typically 10-14 hours post-treatment. For growth hormone studies, sGnRH-A is administered via intraperitoneal injection to fish (e.g., common carp) and blood samples are collected at 1-6 hours post-dose for GH measurement by ELISA.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for sGnRH-A acetate in fish and mammals are limited. As a peptide (MW 1342.5, acetate salt), it is typically administered via injection as it has poor oral bioavailability due to degradation in the gastrointestinal tract. The compound is water-soluble (100 mg/mL in water) and has a relatively short plasma half-life (minutes to hours depending on species). Peak plasma concentrations are achieved within 30-60 minutes post-injection.
|
| Toxicity/Toxicokinetics |
Toxicity data for sGnRH-A acetate are limited. The compound is approved for veterinary use in some countries as a reproductive management tool in aquaculture and livestock production. As a GnRH analogue, potential adverse effects include ovarian hyperstimulation and hormonal imbalances at suprapharmacological doses. For research use, standard safety precautions should be followed. Not for human therapeutic use.
|
| References |
|
| Additional Infomation |
sGnRH-A acetate is a veterinary and research compound used primarily in aquaculture and reproductive biology studies. It is an analogue of salmon GnRH with a substitution of D-arginine at position 6 to enhance potency and resistance to proteolytic degradation. The compound has not been approved for human clinical use but is valuable as a research tool for studying GnRH physiology, GH regulation, and reproductive endocrinology. Store as powder at -20degC to -80degC.
|
| Molecular Formula |
C66H87N17O14
|
|---|---|
| Molecular Weight |
1342.50
|
| Related CAS # |
sGnRH-A;96497-82-4
|
| Appearance |
White to off-white solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~100 mg/mL (~74.49 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.7449 mL | 3.7244 mL | 7.4488 mL | |
| 5 mM | 0.1490 mL | 0.7449 mL | 1.4898 mL | |
| 10 mM | 0.0745 mL | 0.3724 mL | 0.7449 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
