| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| Other Sizes |
| Targets |
L-Methionine-13C5 targets methionine metabolic pathways, including the S-adenosylmethionine (SAM) and homocysteine pathways. It is used to trace methionine metabolism and to study conditions such as homocystinuria and cardiovascular disease. As an essential amino acid, methionine is vital for protein synthesis and various metabolic processes.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, L-Methionine-13C5 is used in cell culture experiments for stable isotope labeling by amino acids in cell culture (SILAC) workflows. The complete 13C5 labeling allows for unambiguous metabolic flux tracing through SAM and homocysteine pathways. It is also used as an internal standard in LC-MS/MS assays to correct for matrix effects and ensure accurate quantification of methionine in biological samples. |
| ln Vivo |
In vivo, L-Methionine-13C5 is used in metabolic flux studies to trace the incorporation and metabolism of methionine in animal models. The labeled compound is administered and its distribution and conversion to downstream metabolites such as SAM and homocysteine are monitored. This approach provides insights into methionine metabolism and its role in various physiological and pathological conditions.
|
| Enzyme Assay |
In vitro enzyme/receptor binding assays using L-Methionine-13C5 typically involve LC-MS/MS-based activity assays for enzymes in the methionine cycle, such as methionine adenosyltransferase. The labeled substrate is used to measure enzyme activity by tracking the conversion to labeled products. The compound also serves as an internal standard to correct for matrix effects in complex biological samples.
|
| Cell Assay |
In vitro cell experiments with L-Methionine-13C5 involve culturing cells in media containing the labeled amino acid for SILAC proteomics. Cells incorporate the heavy isotope into newly synthesized proteins over several passages. The labeled proteins are then analyzed by mass spectrometry for quantitative proteomic comparisons. The compound is also used in cell-based metabolic studies to trace methionine utilization and flux.
|
| Animal Protocol |
In vivo animal experiments with L-Methionine-13C5 involve administering the labeled compound to animals, typically via oral gavage or intravenous injection. Blood, tissue, or urine samples are collected at various time points and analyzed by LC-MS/MS to measure the incorporation of the labeled isotope into methionine and its metabolites. This approach is used to study methionine metabolism, protein synthesis rates, and the effects of nutritional or pharmacological interventions.
|
| ADME/Pharmacokinetics |
L-Methionine-13C5 is used as an internal standard to correct for matrix effects, ionization suppression, and analyte loss during sample preparation in LC-MS/MS and GC-MS assays. The complete 13C5 labeling provides a robust M+5 mass shift that ensures baseline separation from the natural isotopic envelope. The compound is supplied with verified isotopic purity (≥99 atom% 13C) to meet regulated bioanalysis requirements.
|
| Toxicity/Toxicokinetics |
Toxicological data for L-Methionine-13C5 are limited, as the compound is used primarily as an analytical standard and research tool. L-Methionine itself is an essential amino acid that is generally recognized as safe at physiological concentrations. The stable isotope-labeled version is expected to have a similar safety profile and is not intended for therapeutic use.
|
| References |
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216.
|
| Additional Infomation |
L-Methionine-13C5 is a research-use-only compound with no approved therapeutic indications or clinical trials. Its primary application is as an internal standard in targeted metabolomics, for tracing methionine metabolic flux, and in SILAC workflows for quantitative proteomics. The compound enables matrix-independent quantification in human plasma and serum, making it an essential tool for bioanalysis.
|
| Molecular Formula |
13C5H11NO2S
|
|---|---|
| Molecular Weight |
154.17
|
| Exact Mass |
154.067
|
| CAS # |
202326-57-6
|
| Related CAS # |
L-Methionine;63-68-3
|
| PubChem CID |
102468411
|
| Appearance |
White to off-white solid powder
|
| LogP |
-1.9
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
9
|
| Complexity |
97
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
[13CH3]S[13CH2][13CH2][13C@@H]([13C](=O)O)N
|
| InChi Key |
FFEARJCKVFRZRR-JRGPAWSWSA-N
|
| InChi Code |
InChI=1S/C5H11NO2S/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1/i1+1,2+1,3+1,4+1,5+1
|
| Chemical Name |
(2S)-2-amino-4-(113C)methylsulfanyl(1,2,3,4-13C4)butanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 50 mg/mL (324.32 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.4863 mL | 32.4317 mL | 64.8635 mL | |
| 5 mM | 1.2973 mL | 6.4863 mL | 12.9727 mL | |
| 10 mM | 0.6486 mL | 3.2432 mL | 6.4863 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
