| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
L-Methionine-13C,d3 does not have a specific pharmacological target as it is a stable isotope-labeled tracer rather than a drug. L-Methionine itself is an essential amino acid involved in protein synthesis, methylation reactions, and sulfur metabolism. It serves as a strong hepatic detoxifier and liver protectant. The labeled form is used to trace methionine metabolism in biological systems.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro activity of L-Methionine-13C,d3 is measured as its utility as a tracer in metabolic studies. In cell culture, the labeled amino acid is incorporated into proteins and metabolic intermediates, allowing for tracking via mass spectrometry. Its "activity" is reflected in its metabolic incorporation and its ability to serve as a probe for studying methionine metabolism, methylation, and sulfur amino acid pathways. |
| ln Vivo |
In vivo, L-Methionine-13C,d3 is used to study methionine metabolism, protein synthesis, and methylation reactions in living organisms. Administered orally or intravenously, the labeled amino acid is incorporated into proteins or metabolized through transmethylation and transsulfuration pathways. These studies provide quantitative data on methionine kinetics and its role in one-carbon metabolism and liver function.
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| Enzyme Assay |
In vitro enzyme assays with L-Methionine-13C,d3 typically involve studying enzymes of methionine metabolism such as methionine adenosyltransferase, methionine synthase, and cystathionine beta-synthase. The labeled substrate is incubated with enzyme preparations, and the reaction products are analyzed by mass spectrometry. These assays provide mechanistic insights into enzyme kinetics and the regulation of methionine metabolism.
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| Cell Assay |
In vitro cell culture experiments with L-Methionine-13C,d3 involve supplementing cell culture media with the labeled amino acid, often in methionine-free or defined media. Cells are cultured to allow incorporation of the label into proteins and metabolites. Following incubation, cells are harvested, and proteins or metabolites are extracted for analysis by mass spectrometry. These experiments are used in SILAC-based proteomics and metabolic flux analysis.
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| Animal Protocol |
In vivo animal experiments with L-Methionine-13C,d3 typically involve administering the labeled compound via oral gavage, intravenous injection, or dietary incorporation to rodents. Blood, tissues (liver, brain, muscle), and excreta are collected at various time points. Isotopic enrichment of methionine and its metabolites is measured by mass spectrometry. These studies provide quantitative data on whole-body methionine metabolism and one-carbon metabolism.
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| ADME/Pharmacokinetics |
The pharmacokinetic (PK) properties of L-Methionine-13C,d3 are essentially identical to those of natural L-methionine. L-methionine is absorbed from the gastrointestinal tract via amino acid transporters, distributed throughout the body, and utilized in protein synthesis or metabolized. It has a relatively short plasma half-life due to rapid clearance and utilization. The isotope labels allow for precise tracking of the compound's distribution and metabolism.
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| Toxicity/Toxicokinetics |
L-Methionine-13C,d3 has a low toxicity profile since it is a naturally occurring essential amino acid. The isotope labels (13C and D) are stable, non-radioactive isotopes and do not introduce additional toxicity. At normal physiological concentrations, L-methionine is safe and well-tolerated. For research use, standard laboratory safety practices are sufficient.
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| References | |
| Additional Infomation |
L-Methionine-13C,d3 is a stable isotope-labeled form of the essential amino acid methionine, where one carbon atom is replaced with 13C and three hydrogen atoms are substituted with deuterium. It is used as a tracer in metabolic studies and as a SILAC amino acid for proteomics research. The compound is not a drug and has no therapeutic indications. It is available for laboratory research use only.
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| Molecular Formula |
C413CH8D3NO2S
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|---|---|
| Molecular Weight |
153.22
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| Exact Mass |
153.073
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| CAS # |
73488-65-0
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| Related CAS # |
L-Methionine;63-68-3
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| PubChem CID |
15556487
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| Appearance |
White to off-white solid powder
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| Melting Point |
273ºC (dec.)(lit.)
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| LogP |
0.851
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
9
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| Complexity |
97
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H][13C]([2H])([2H])SCC[C@@H](C(=O)O)N
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| InChi Key |
FFEARJCKVFRZRR-ZIHWZSJBSA-N
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| InChi Code |
InChI=1S/C5H11NO2S/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1/i1+1D3
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| Chemical Name |
(2S)-2-amino-4-(trideuterio(113C)methylsulfanyl)butanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5266 mL | 32.6328 mL | 65.2656 mL | |
| 5 mM | 1.3053 mL | 6.5266 mL | 13.0531 mL | |
| 10 mM | 0.6527 mL | 3.2633 mL | 6.5266 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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