| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| Other Sizes |
| Targets |
2-NP-AMOZ has no intrinsic pharmacological target. The parent drug, Furaltadone, is an antibiotic that targets bacterial cell wall synthesis. AMOZ is its stable metabolite. This analytical standard is not administered to animals; it is a chemical derivative used specifically for residue analysis. Its "target" is the detection of AMOZ in complex biological matrices for food safety testing.
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|---|---|
| ln Vitro |
2-NP-AMOZ is not a pharmacologically active compound and is not used to measure in vitro activity. It is a derivatized standard for analytical chemistry. The parent compound, AMOZ, is a metabolite of the antibiotic Furaltadone. The purpose of this standard is not to assess activity but to enable precise detection of prohibited residues in animal tissues, such as chicken, pork, and fish.
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| ln Vivo |
2-NP-AMOZ is an analytical standard and has no direct in vivo activity. It is used as a reference to monitor and quantify the presence of AMOZ residues in animal tissues for regulatory compliance and food safety. When an animal is treated with Furaltadone, the drug is rapidly metabolized to AMOZ, which binds to tissue proteins. This derivative is used to detect those bound residues.
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| Enzyme Assay |
2-NP-AMOZ is used as a certified reference standard for non-cellular analytical method development. For LC-MS/MS analysis, the standard is dissolved in an organic solvent like methanol or acetonitrile to prepare a stock solution. This solution is then diluted to a series of known concentrations to create a calibration curve. It is also used as a quality control standard to verify the performance of the analytical instrument and the sample preparation method, ensuring accurate identification and quantification of AMOZ residues.
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| Cell Assay |
2-NP-AMOZ is not used in conventional cell culture experiments. Its application is entirely analytical. To test the effectiveness of an analytical method, known as "spike-recovery," a known amount of the 2-NP-AMOZ standard is added (spiked) to a blank tissue sample homogenate (e.g., chicken liver) before the extraction and derivatization steps. The recovered amount is then measured, and the recovery rate is calculated to validate the accuracy of the method.
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| Animal Protocol |
2-NP-AMOZ is not administered to animals for research purposes. However, it is a critical tool in studies involving animal safety and food production. For example, in a study where animals are treated with Furaltadone, tissue samples are collected. These samples are processed, and the 2-NP-AMOZ standard is used to quantify the amount of AMOZ residues. The concentration data obtained is used to determine the withdrawal period (the time required for drug residues to fall to safe levels) for the antibiotic.
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| ADME/Pharmacokinetics |
2-NP-AMOZ is an analytical standard and has no pharmacokinetic parameters. The parent drug, Furaltadone, is rapidly metabolized to AMOZ in vivo, which binds to tissue proteins and has a long residence time in the body. The withdrawal period is set based on the depletion of these bound residues, which is measured using this analytical standard.
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| Toxicity/Toxicokinetics |
2-NP-AMOZ is an analytical standard and is handled in very small quantities (micrograms to milligrams). The parent compound, AMOZ, is a metabolite of a carcinogenic antibiotic, and residues are regulated to be at very low (non-detectable) levels in food. As a research chemical, it is considered a potential health hazard and should be handled with strict precautions, including the use of disposable gloves, a chemical fume hood, and proper disposal procedures to avoid any exposure.
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| References |
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| Additional Infomation |
2-NP-AMOZ is not a drug but an analytical reference standard. Its sole purpose is to ensure the safety of the food supply. It is used by government regulatory agencies, food safety laboratories, and pharmaceutical companies to test for the illegal use of nitrofuran antibiotics (Furaltadone) in food-producing animals. This ensures that meat, milk, and other animal products are free from prohibited carcinogenic residues before they reach the consumer.
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| Molecular Formula |
C15H18N4O5
|
|---|---|
| Molecular Weight |
334.33
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| Exact Mass |
334.127
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| CAS # |
183193-59-1
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| Related CAS # |
2-NP-AMOZ-d5;1173097-59-0
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| PubChem CID |
53393538
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
485.2±53.0 °C at 760 mmHg
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| Flash Point |
247.3±30.9 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.652
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| LogP |
0.89
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
24
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| Complexity |
489
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C(C(=C1)/C=N/N2CC(CN3CCOCC3)OC2=O)[N+](=O)[O-]
|
| InChi Key |
PCYAMVONOKWOLP-CXUHLZMHSA-N
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| InChi Code |
InChI=1S/C15H18N4O5/c20-15-18(11-13(24-15)10-17-5-7-23-8-6-17)16-9-12-3-1-2-4-14(12)19(21)22/h1-4,9,13H,5-8,10-11H2/b16-9+
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| Chemical Name |
5-(morpholin-4-ylmethyl)-3-[(E)-(2-nitrophenyl)methylideneamino]-1,3-oxazolidin-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9911 mL | 14.9553 mL | 29.9106 mL | |
| 5 mM | 0.5982 mL | 2.9911 mL | 5.9821 mL | |
| 10 mM | 0.2991 mL | 1.4955 mL | 2.9911 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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