| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
2-NP-AMOZ-d5 does not have a specific biological target but is used as an analytical standard. AMOZ is the tissue-bound metabolite of furaltadone, a nitrofuran antibiotic that has been banned in food-producing animals due to concerns about its carcinogenic potential. 2-NP-AMOZ-d5 is used to detect protein-bound AMOZ residues in food products. As a deuterated analog, it behaves identically to unlabeled 2-NP-AMOZ in analytical procedures but can be distinguished by mass spectrometry, allowing for accurate quantification of AMOZ residues.
|
|---|---|
| ln Vitro |
In vitro, 2-NP-AMOZ-d5 is used as an internal standard in analytical assays for the detection of AMOZ residues in food samples. The compound is added to samples during the extraction and derivatization process, and its recovery is monitored to correct for losses during sample preparation. Its in vitro activity is purely analytical; it does not exert any pharmacological or toxicological effects. The compound's stability and recovery are assessed during method validation studies. It is available as a certified reference material for analytical research.
|
| ln Vivo |
In vivo, 2-NP-AMOZ-d5 is not administered as a therapeutic agent. It is used as an analytical standard for monitoring AMOZ residues in food products derived from animals that may have been treated with furaltadone. The compound's in vivo relevance is limited to its use in food safety testing and regulatory compliance. The deuterated label allows for accurate quantification of AMOZ residues by LC-MS/MS, ensuring that food products meet safety standards.
|
| Enzyme Assay |
The in vitro analytical assay for 2-NP-AMOZ-d5 involves the extraction and derivatization of AMOZ from food samples (e.g., shrimp, meat, poultry). Samples are hydrolyzed with acid to release protein-bound AMOZ, followed by derivatization with 2-nitrobenzaldehyde to form 2-NP-AMOZ. The deuterated internal standard (2-NP-AMOZ-d5) is added before extraction. The derivatized samples are extracted with organic solvent and analyzed by LC-MS/MS. The compound is quantified by monitoring specific mass transitions for the deuterated and unlabeled compounds. Calibration curves are prepared using known concentrations of 2-NP-AMOZ with a fixed concentration of the deuterated internal standard. The method is validated for accuracy, precision, and linearity according to regulatory guidelines.
|
| Cell Assay |
For in vitro cellular assays, 2-NP-AMOZ-d5 is not typically used, as it is an analytical standard rather than a compound with biological activity. Its primary application is in analytical chemistry and food safety testing. The compound may be used in cell culture studies to track the uptake and metabolism of AMOZ or 2-NP-AMOZ, but such applications are rare. The compound is typically used as an internal standard in LC-MS/MS analysis.
|
| Animal Protocol |
For in vivo studies, 2-NP-AMOZ-d5 is not administered to animals as a therapeutic agent. It is used in food safety testing to monitor AMOZ residues in animal-derived food products. The compound's in vivo relevance is limited to its use as an analytical standard for regulatory compliance. No animal procedures are typically performed with this compound itself, as it is used in the analysis of samples from animals that may have been treated with furaltadone.
|
| ADME/Pharmacokinetics |
The pharmacokinetic properties of 2-NP-AMOZ-d5 are not relevant, as it is an analytical standard rather than a therapeutic agent. The compound has a molecular weight of 339.36. When used as an internal standard, its behavior in analytical procedures is identical to that of unlabeled 2-NP-AMOZ. The compound is stable under recommended storage conditions and is used for analytical method development, validation, and quality control.
|
| Toxicity/Toxicokinetics |
The toxicology of 2-NP-AMOZ-d5 is not relevant, as it is an analytical standard used at trace levels in laboratory analysis. The compound is not intended for human or animal consumption. Nitrofuran antibiotics, including furaltadone, have been banned in food-producing animals due to concerns about carcinogenicity. The deuterated internal standard is used to ensure accurate quantification of AMOZ residues in food safety testing. The compound should be handled with appropriate laboratory safety precautions.
|
| References |
|
| Additional Infomation |
2-NP-AMOZ-d5 is a deuterium-labeled internal standard for the quantification of AMOZ, a metabolite of the nitrofuran antibiotic furaltadone. It is used in food safety testing to monitor AMOZ residues in animal-derived food products. The compound is not approved for human use and is intended for analytical research purposes only. It is available as a high-purity analytical standard for laboratory use.
|
| Molecular Formula |
C15H18N4O5
|
|---|---|
| Molecular Weight |
339.358031749725
|
| Exact Mass |
339.159
|
| CAS # |
1173097-59-0
|
| Related CAS # |
2-NP-AMOZ;183193-59-1
|
| PubChem CID |
71434098
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
485.2±53.0 °C at 760 mmHg
|
| Flash Point |
247.3±30.9 °C
|
| Vapour Pressure |
0.0±1.2 mmHg at 25°C
|
| Index of Refraction |
1.652
|
| LogP |
0.89
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
24
|
| Complexity |
489
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C(C(=C1)/C=N/N2C([2H])([2H])C([2H])(C([2H])([2H])N3CCOCC3)OC2=O)[N+](=O)[O-]
|
| InChi Key |
PCYAMVONOKWOLP-DTSMCWKPSA-N
|
| InChi Code |
InChI=1S/C15H18N4O5/c20-15-18(11-13(24-15)10-17-5-7-23-8-6-17)16-9-12-3-1-2-4-14(12)19(21)22/h1-4,9,13H,5-8,10-11H2/i10D2,11D2,13D
|
| Chemical Name |
4,4,5-trideuterio-5-[dideuterio(morpholin-4-yl)methyl]-3-[(2-nitrophenyl)methylideneamino]-1,3-oxazolidin-2-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9467 mL | 14.7336 mL | 29.4672 mL | |
| 5 mM | 0.5893 mL | 2.9467 mL | 5.8934 mL | |
| 10 mM | 0.2947 mL | 1.4734 mL | 2.9467 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
