| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| 2g | |||
| Other Sizes |
| Targets |
L-Leucine-1-13C targets the same biological pathways as L-Leucine. L-Leucine acts as a nutrient signal to stimulate protein synthesis. It activates the mammalian target of rapamycin (mTOR) kinase, which regulates cell growth. L-Leucine is involved in protein synthesis, metabolic regulation, and as a precursor for other molecules. The 13C-label allows for the tracing of leucine metabolism in vivo and in vitro.
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| ln Vitro |
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has drawn attention due to its potential to impact drug pharmacokinetic and metabolic profiles[1].
L-Leucine-1-13C is used in vitro as a tracer to study leucine metabolism. It is used to measure protein synthesis rates, amino acid uptake, and metabolic flux. The 13C-label allows for the quantification of leucine and its metabolites using mass spectrometry or NMR spectroscopy. |
| ln Vivo |
L-Leucine-1-13C is used in vivo as a tracer to study leucine metabolism and protein turnover. It is administered to animals or humans, and the incorporation of 13C into proteins or metabolites is measured. This allows for the determination of protein synthesis rates, amino acid kinetics, and metabolic flux.
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| Enzyme Assay |
L-Leucine-1-13C is a stable isotope-labeled compound, and its "activity" is measured by its enrichment and its use as a tracer. In a typical assay, the compound is administered to cells or organisms, and the incorporation of 13C into proteins or metabolites is measured using mass spectrometry or NMR spectroscopy. The rate of incorporation is used to calculate protein synthesis rates or metabolic flux.
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| Cell Assay |
L-Leucine-1-13C is dissolved in aqueous buffers and applied to cells or administered to animals. The concentration used depends on the specific application, but it is typically in the range of 1-100 µM for cell culture or 1-100 mg/kg for animal studies. The incorporation of 13C is then measured.
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| ADME/Pharmacokinetics |
L-Leucine-1-13C has a molecular weight of 132.17 g/mol and a molecular formula of C6H13NO2 (with one 13C atom). It is a stable isotope-labeled compound. The compound is typically stored as a powder at room temperature. L-Leucine-1-13C is also known as L-(1-13C)Leucine.
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| Toxicity/Toxicokinetics |
L-Leucine-1-13C is considered to have low toxicity, consistent with L-Leucine. L-Leucine is an essential amino acid and is generally recognized as safe. No significant toxicity has been reported for the 13C-labeled compound.
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| References |
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| Additional Infomation |
L-Leucine-1-13C is a stable isotope-labeled form of the essential amino acid L-Leucine. It is commonly used in medical, environmental, and industrial research as a tracer. L-Leucine-1-13C is a 13C-labeled L-Leucine that significantly enhances the affinity of GLP-4. L-Leucine acts as a nutrient signal to stimulate protein synthesis and activates the mTOR signaling pathway.
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| Molecular Formula |
C513CH13NO2
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|---|---|
| Molecular Weight |
132.16
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| Exact Mass |
132.098
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| Elemental Analysis |
C, 55.28; H, 9.91; N, 10.60; O, 24.21
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| CAS # |
74292-94-7
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| Related CAS # |
L-Leucine;61-90-5
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| PubChem CID |
135315
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| Appearance |
Solid powder
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| Density |
1.035g/cm3
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| Melting Point |
>300ºC(lit.)
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| Index of Refraction |
1.462
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| LogP |
1.144
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
9
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| Complexity |
101
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(C)CC(C(=O)O)N
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| InChi Key |
ROHFNLRQFUQHCH-SANWUMGISA-N
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| InChi Code |
InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i6+1
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| Chemical Name |
(2S)-2-amino-4-methyl(113C)pentanoic acid
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| Synonyms |
(1-13C)Leucine; L-(1-13C)Leucine; L-Leucine-1-13C
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: ~5 mg/mL (~37.83 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.5666 mL | 37.8329 mL | 75.6659 mL | |
| 5 mM | 1.5133 mL | 7.5666 mL | 15.1332 mL | |
| 10 mM | 0.7567 mL | 3.7833 mL | 7.5666 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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