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| Other Sizes |
| Targets |
L-Asparagine does not have a specific pharmacological target. It is a naturally occurring proteinogenic amino acid involved in nitrogen metabolism and protein synthesis. As a non-essential amino acid, it participates in neurological and metabolic regulation of tissues. L-Asparagine is also used as a local anesthetic, with its structure featuring a thiophene ring instead of a benzene ring which distinguishes it from other anesthetics. The compound serves as a building block for protein and peptide synthesis and is essential for cell culture applications. Its role in nitrogen metabolism makes it important for maintaining cellular homeostasis.
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| ln Vitro |
In vitro, L-Asparagine is used as a versatile reagent for cell culture media preparation, biochemical assays, nutritional studies, and pharmaceutical or biotechnological applications. It is a polar, uncharged amino acid derivative that plays a central role in nitrogen metabolism. The compound features an amide functional group that enables participation in protein and peptide synthesis. In biomanufacturing, L-asparagine is used in cell culture systems for the production of therapeutic recombinant proteins and monoclonal antibodies. As a non-essential amino acid, it participates in neurological and metabolic regulation of tissues. Its in vitro activity is primarily related to its role as a nutrient and metabolic substrate rather than specific pharmacological effects.
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| ln Vivo |
In vivo, L-Asparagine is a naturally occurring proteinogenic amino acid that plays a central role in nitrogen metabolism. As a non-essential amino acid, it participates in neurological and metabolic regulation of tissues. The compound is used in biomanufacturing cell culture systems and as a nutritional supplement. L-Asparagine is also used as a local anesthetic. In the body, it is involved in the synthesis of proteins and other nitrogen-containing compounds. It is metabolized through the asparagine synthetase pathway and is excreted primarily as urea. The compound is generally recognized as safe for use in food and pharmaceutical applications.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cellular) assays are not applicable to L-Asparagine, as it is a naturally occurring amino acid rather than a drug. However, the compound may be used as a substrate in enzyme activity assays for asparaginase, which catalyzes the hydrolysis of asparagine to aspartic acid and ammonia. Enzyme activity is measured by monitoring ammonia production using colorimetric or coupled enzyme assays. L-Asparagine may also be used in assays for asparagine synthetase, which catalyzes the synthesis of asparagine from aspartic acid and glutamine. These assays are relevant to studies of amino acid metabolism and to the development of asparaginase-based therapies for leukemia.
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| Cell Assay |
In vitro cellular experiments with L-Asparagine are performed using various cell lines to assess its effects on cell growth and metabolism. Cells are cultured in appropriate media (e.g., DMEM or RPMI 1640 with 10% FBS) with varying concentrations of L-Asparagine. Cell proliferation and viability are assessed using MTT or CellTiter-Glo assays. L-Asparagine is an essential nutrient for many cell types and is commonly included in cell culture media formulations. Its depletion by asparaginase is used as a therapeutic strategy for acute lymphoblastic leukemia. The compound's effects on protein synthesis and nitrogen metabolism may be studied using metabolic labeling or biochemical assays. Cells are maintained at 37°C in 5% CO₂ with appropriate media supplements.
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| Animal Protocol |
In vivo animal studies with L-Asparagine are not typically performed for therapeutic development, as it is a naturally occurring amino acid. However, studies may be conducted to evaluate the effects of asparagine depletion or supplementation in various disease models. For example, asparaginase, which depletes asparagine, is used in the treatment of acute lymphoblastic leukemia. Animal models of leukemia are used to study the efficacy of asparaginase and the role of asparagine in tumor growth. L-Asparagine supplementation studies may be conducted to assess its effects on metabolism and tissue function. The compound is also used in nutritional studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-Asparagine indicate that it is a naturally occurring amino acid that is well-absorbed and distributed throughout the body. The compound has molecular formula C₄H₁₀N₂O₄ and molecular weight 150.13 g/mol. It appears as a white crystalline powder. L-Asparagine is soluble in water and is stable in its hydrated crystalline form. It is metabolized through the asparagine synthetase pathway and is excreted primarily as urea. As a non-essential amino acid, it is synthesized endogenously and obtained from the diet. Storage: follow manufacturer's guidelines.
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| Toxicity/Toxicokinetics |
Toxicological information for L-Asparagine indicates that it is a naturally occurring amino acid that is generally recognized as safe for use in food, pharmaceutical, and biomanufacturing applications. The compound is a polar, uncharged amino acid derivative. Standard safety precautions for handling chemicals apply, including use of personal protective equipment (gloves, safety goggles, lab coat) and working in a well-ventilated area. The compound is for research use and is also used in biomanufacturing and nutritional applications. Purity: ≥98%.
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| Additional Infomation |
See also: Asparagine (note moved to).
L-Asparagine monohydrate (CAS 5794-13-8) is a naturally occurring proteinogenic amino acid. The compound has molecular formula C₄H₁₀N₂O₄ and molecular weight 150.13 g/mol. L-Asparagine is a polar, uncharged amino acid derivative that plays a central role in nitrogen metabolism. It is used in biomanufacturing cell culture systems for the production of therapeutic recombinant proteins and monoclonal antibodies. Also known as L(+)-Asparagine monohydrate and (S)-(+)-2-Aminosuccinamic acid monohydrate. Purity: ≥98%. |
| Molecular Formula |
C4H10N2O4
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| Molecular Weight |
150.1332
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| Exact Mass |
150.064
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| CAS # |
5794-13-8
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| Related CAS # |
L-Asparagine;70-47-3;L-Asparagine-13C4,15N2 monohydrate;202406-87-9;L-Asparagine-amide-15N monohydrate;204451-47-8;L-Asparagine-13C4,15N2,d3 monohydrate;2483829-30-5;L-Asparagine-15N2,d3 monohydrate;L-Asparagine-1,2,3,4-13C4 monohydrate;768348-44-3
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| PubChem CID |
170358
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| Appearance |
White to off-white solid powder
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| Density |
1,543 g/cm3
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| Boiling Point |
438ºC at 760 mmHg
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| Melting Point |
233-235 °C(lit.)
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| Flash Point |
218.7ºC
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| Index of Refraction |
31 ° (C=10, HCl)
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
10
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| Complexity |
134
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| Defined Atom Stereocenter Count |
1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~20 mg/mL (~133.22 mM)
DMSO :< 1 mg/mL |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6609 mL | 33.3045 mL | 66.6089 mL | |
| 5 mM | 1.3322 mL | 6.6609 mL | 13.3218 mL | |
| 10 mM | 0.6661 mL | 3.3304 mL | 6.6609 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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