| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
No specific drug target; serves as a stable isotope-labeled tracer and internal standard for asparagine metabolism studies.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The non-labeled L-asparagine monohydrate is a non-essential amino acid involved in the metabolic control of cell functions in nerve and brain tissue. As a stable isotope-labeled compound, L-Asparagine-13C4,15N2 monohydrate exhibits identical chemical properties to unlabeled asparagine but provides a distinct mass signature for precise quantification by LC-MS/MS. |
| ln Vivo |
As a stable isotope-labeled internal standard, in vivo activity is not assessed. The non-labeled L-asparagine is an amino acid essential for protein synthesis and nitrogen transport. It plays a role in the metabolic control of cell function in nerve and brain tissue and is involved in the urea cycle. L-asparagine is also used clinically as a component of parenteral nutrition.
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| Enzyme Assay |
Receptor binding assays are not applicable. For analytical use, standard LC-MS/MS protocols involve preparing calibration standards and quality control samples containing L-Asparagine-13C4,15N2 monohydrate as an internal standard. Sample extraction from biological matrices (plasma, urine, or tissue) is followed by protein precipitation, derivatization if required, and analysis by LC-MS/MS with MRM detection.
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| Cell Assay |
Not applicable as this is an analytical internal standard. For cell culture studies, labeled L-asparagine can be added to culture media at tracer concentrations (e.g., 0.1-2 mM). Cells are incubated for 1-24 hours to allow incorporation into proteins and metabolites. Cellular extracts are analyzed by LC-MS to trace 13C and 15N incorporation into metabolic pathways.
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| Animal Protocol |
In vivo metabolic flux studies using L-Asparagine-13C4,15N2 monohydrate involve intravenous or oral administration of the labeled amino acid to rodents (mice or rats). Blood samples are collected at multiple time points (0-6 hours) and analyzed by LC-MS/MS. Tissues including liver, kidney, and brain may be harvested for isotopic enrichment analysis to study asparagine metabolism and distribution.
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| ADME/Pharmacokinetics |
As an analytical standard, pharmacokinetic properties are not directly assessed. The labeled compound follows the same metabolic fate as unlabeled L-asparagine, which is rapidly distributed throughout the body and actively transported into cells, where it is incorporated into proteins or deaminated by asparaginase. The plasma half-life of L-asparagine is relatively short (approximately 15-30 minutes).
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| Toxicity/Toxicokinetics |
Toxicity data for the labeled compound are not available as it is used only as an analytical standard at tracer concentrations. The non-labeled L-asparagine is a naturally occurring amino acid considered safe at physiological concentrations. At very high doses, L-asparagine is generally well-tolerated. However, asparaginase (which depletes asparagine) is used therapeutically in cancer treatment, indicating that asparagine is essential for certain tumor cells.
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| References | |
| Additional Infomation |
L-Asparagine-13C4,15N2 monohydrate is a stable isotope-labeled compound used exclusively for research and bioanalytical applications. It is particularly valuable as an internal standard for the quantification of L-asparagine in pharmacokinetic studies of asparaginase drugs (e.g., pegaspargase) used in acute lymphoblastic leukemia treatment. This compound is not a therapeutic agent and has no approved clinical indications.
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| Molecular Formula |
C4H10N2O4
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|---|---|
| Molecular Weight |
150.133201122284
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| Exact Mass |
156.071
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| CAS # |
202406-87-9
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| Related CAS # |
L-Asparagine monohydrate;5794-13-8
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| PubChem CID |
117064246
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
10
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| Complexity |
134
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| Defined Atom Stereocenter Count |
1
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| SMILES |
OC([C@H](CC(N)=O)N)=O.O
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| InChi Key |
RBMGJIZCEWRQES-KNBQNQHASA-N
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| InChi Code |
InChI=1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1;
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| Chemical Name |
(2S)-2,4-bis(15N)(azanyl)-4-oxo(1,2,3,4-13C4)butanoic acid;hydrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6609 mL | 33.3045 mL | 66.6089 mL | |
| 5 mM | 1.3322 mL | 6.6609 mL | 13.3218 mL | |
| 10 mM | 0.6661 mL | 3.3304 mL | 6.6609 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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