| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| Targets |
Asparagine is a substrate for protein synthesis and is involved in nitrogen metabolism. It participates in the metabolism of toxic ammonia in the body through an amidation reaction catalyzed by asparagine synthase, whereby ammonia is linked to aspartic acid. Asparagine is critical for the production of the body's proteins, enzymes, and muscle tissue. It is an essential amino acid for leukemic cells.
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|---|---|
| ln Vitro |
In vitro, L-Asparagine (0.03 mM) can reverse the proliferation decline of six human cancer cells induced by ASNS siRNA knockdown. L-Asparagine (10 mM, 4 h) can stimulate ODC activity and proliferation in IPEC-J2 cells. L-Asparagine is a substrate for L-Asparaginase, a potent anti-leukemic enzyme that promotes asparagine and glutamine depletion and inhibits protein biosynthesis in lymphoblasts.
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| ln Vivo |
In vivo, asparagine is used for nutritional supplementation and for treating dietary shortage or imbalance. It is a non-essential amino acid commonly occurring in the L-form, found in animals and plants. Asparagine may be a neurotransmitter. It is involved in various biological processes including protein synthesis.
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| Enzyme Assay |
Cellular studies with asparagine typically involve cell culture media supplementation or depletion. The effects of asparagine on cell proliferation are assessed by adding it to cultures at various concentrations (e.g., 0.03 mM to 10 mM) and measuring cell growth. The role of asparagine in protein synthesis can be assessed by monitoring the incorporation of radiolabeled asparagine into proteins. In leukemia research, asparagine depletion by L-asparaginase is used to study its effects on lymphoblast survival.
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| Cell Assay |
Asparagine has a molecular formula of C4H8N2O3 and a molecular weight of 132.12 g/mol. Its CAS number is 70-47-3. It is a non-essential amino acid. It appears as white crystals or crystalline powder and is soluble in water. It should be stored at room temperature.
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| Animal Protocol |
In 90-day constant oral studies of rats, asparagine caused weight loss, effects on phosphatases, lipids, and transaminases, changes in brain, kidney, and testicular weights, hyperglycemia, and changes in potassium. It may cause irritation. As with all amino acids, high doses may have adverse effects. Standard safety precautions should be taken when handling asparagine.
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| ADME/Pharmacokinetics |
Asparagine is a non-essential amino acid involved in protein synthesis, cellular signaling, and nitrogen metabolism. It is critical for the production of the body's proteins, enzymes, and muscle tissue. It plays a critical role in the biosynthesis of glycoproteins and the proper functioning of the nervous system. It is used for nutritional supplementation and for treating dietary shortage or imbalance. Asparagine is also used in research to study protein metabolism and leukemia.
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| Toxicity/Toxicokinetics |
Toxicity Summary
Asparagine is a non-essential amino acid that participates in the metabolism of toxic ammonia in the body through an amidation reaction caused by asparagine synthase, whereby ammonia is linked to aspartic acid. Asparagine is also a structural component of many proteins. |
| References |
|
| Additional Infomation |
Pharmacodynamics
Asparagine is a non-essential amino acid. It is crucial for the production of proteins, enzymes, and muscle tissue in the human body. It is claimed that supplementing with this amino acid can balance nervous system function. |
| Molecular Formula |
C4H8N2O3
|
|---|---|
| Molecular Weight |
132.1179
|
| Exact Mass |
132.053
|
| CAS # |
70-47-3
|
| Related CAS # |
28088-48-4
|
| PubChem CID |
6267
|
| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
438.0±40.0 °C at 760 mmHg
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| Melting Point |
235 °C (dec.)(lit.)
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| Flash Point |
218.7±27.3 °C
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| Vapour Pressure |
0.0±2.3 mmHg at 25°C
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| Index of Refraction |
1.533
|
| LogP |
-1.51
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
9
|
| Complexity |
134
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
C([C@@H](C(=O)O)N)C(=O)N
|
| InChi Key |
DCXYFEDJOCDNAF-REOHCLBHSA-N
|
| InChi Code |
InChI=1S/C4H8N2O3/c5-2(4(8)9)1-3(6)7/h2H,1,5H2,(H2,6,7)(H,8,9)/t2-/m0/s1
|
| Chemical Name |
(2S)-2,4-diamino-4-oxobutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~6.67 mg/mL (~50.48 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (75.69 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.5689 mL | 37.8444 mL | 75.6888 mL | |
| 5 mM | 1.5138 mL | 7.5689 mL | 15.1378 mL | |
| 10 mM | 0.7569 mL | 3.7844 mL | 7.5689 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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