Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Purity: =99.73%
ISRIB (mixed cis- and trans-isomer) is a novel and potent ISR (Integrated Stress Response) Inhibitor. It targets the interactions between eIF2α kinases and elF2B by acting downstream of the PERK signaling pathway. Upon induction of ER stress, ISRIB (IC50 = 5 nM) inhibits the unfolded protein response mediated by eIF2α phosphorylation.
Targets |
ISR; eIF2α
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ln Vitro |
ISRIB inhibits the synthesis of endogenous ATF4, while XBP1 mRNA splicing and production continued. By obstructing signaling via the PERK branch of the UPR, ISRIB keeps cells from regaining ER homeostasis and reduces the survival of ER-stressed cells.[1]
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ln Vivo |
ISRIB exhibits good bioavailability in vivo and favorable properties in pharmacokinetic profiling experiments. By improving fear-associated and spatial learning, ISRIB (0.25 mg/kg i.p.) improves long-term memory in mice.[1]
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Enzyme Assay |
In 96-well plates, ATF4-dGFP-IRES-Cherry reporter-expressing U2OS cells are treated for eight hours with 100 nM thalidomide and 10μM of the selected compounds. Hoechst 33,258 is used to stain cells, and an automated microscope is used to view the results. The INCell Developer Toolbox Software, version 1.9, is used for data acquisition and image analysis. Repurchased for additional analysis are compounds that prevent the ATF4-dGFP reporter from being induced, do not prevent the accumulation of mCherry downstream of the IRES, and are judged non-toxic based on the number of cells counted through nuclei.
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Animal Protocol |
Male C57BL/6J mice
0.25 mg/kg i.p. |
References |
Molecular Formula |
C22H24CL2N2O4
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Molecular Weight |
451.3430
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Exact Mass |
450.1113
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Elemental Analysis |
C, 58.55; H, 5.36; Cl, 15.71; N, 6.21; O, 14.18
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CAS # |
548470-11-7
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Appearance |
Solid powder
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SMILES |
C1CC(CCC1NC(=O)COC2=CC=C(C=C2)Cl)NC(=O)COC3=CC=C(C=C3)Cl
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InChi Key |
HJGMCDHQPXTGAV-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C22H24Cl2N2O4/c23-15-1-9-19(10-2-15)29-13-21(27)25-17-5-7-18(8-6-17)26-22(28)14-30-20-11-3-16(24)4-12-20/h1-4,9-12,17-18H,5-8,13-14H2,(H,25,27)(H,26,28)
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Chemical Name |
2-(4-chlorophenoxy)-N-[4-[[2-(4-chlorophenoxy)acetyl]amino]cyclohexyl]acetamide
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Synonyms |
ISRIB
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 8.3~10 mg/mL (18.5~22.2 mM)
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.2156 mL | 11.0781 mL | 22.1562 mL | |
5 mM | 0.4431 mL | 2.2156 mL | 4.4312 mL | |
10 mM | 0.2216 mL | 1.1078 mL | 2.2156 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.