| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 1g | |||
| Other Sizes |
(+)-Viroallosecurinine is a naturally occurring cytotoxic alkaloid extracted from Securinega virosa. It exhibits antibacterial activity with MIC of 0.48 μg/mL for Ps. Aeruginosa and Staph. aureus.
| ln Vitro |
Viroallosecurinine exhibited potent in vitro antibacterial activity. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined against five bacterial strains using the agar dilution macromethod.
For Pseudomonas aeruginosa, the MIC was 0.048 mg/ml and the MBC was 0.192 mg/ml. For Staphylococcus aureus, the MIC was 0.048 mg/ml and the MBC was 0.098 mg/ml. For Escherichia coli, the MIC was 0.78 mg/ml and the MBC was 1.56 mg/ml. For Enterococcus faecium, the MIC was 0.70 mg/ml and the MBC was 1.56 mg/ml. For Mycobacterium smegmatis, the MIC was 0.096 mg/ml and the MBC was 0.192 mg/ml. The compound was determined to be bactericidal, as the MIC/MBC ratios were less than 1 for all tested bacteria.[1] |
|---|---|
| References | |
| Additional Infomation |
Securinine belongs to the indoleazine class of compounds.
Securinine has been reported to be found in Flueggea suffruticosa, Phyllanthus amarus, and other organisms with relevant data. See also: Phyllanthus amarus top (part). ViroaloSecurinine is one of two major alkaloids isolated from the leaves of Phyllanthus discoideus. It was tested along with the freeze-dried water extract (LWE), alkaloid fraction (AF), water residue (WR), and another major alkaloid, Securinine. The study concluded that the antibacterial activity of these alkaloids may provide a scientific basis for the traditional use of Phyllanthus discoideus in the treatment of infectious diseases. [1] |
| Molecular Formula |
C13H15NO2
|
|---|---|
| Molecular Weight |
217.26400
|
| Exact Mass |
217.11
|
| CAS # |
1857-30-3
|
| Related CAS # |
Allosecurinine;884-68-4
|
| PubChem CID |
442872
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
459.0±45.0 °C at 760 mmHg
|
| Melting Point |
145-147 ºC (hexane acetone)
|
| Flash Point |
197.0±19.6 °C
|
| Vapour Pressure |
0.0±1.1 mmHg at 25°C
|
| Index of Refraction |
1.633
|
| LogP |
0.6
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
16
|
| Complexity |
426
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
C1CCN2C(C1)C34CC2C=CC3=CC(=O)O4
|
| InChi Key |
SWZMSZQQJRKFBP-WZRBSPASSA-N
|
| InChi Code |
InChI=1S/C13H15NO2/c15-12-7-9-4-5-10-8-13(9,16-12)11-3-1-2-6-14(10)11/h4-5,7,10-11H,1-3,6,8H2/t10-,11-,13+/m1/s1
|
| Chemical Name |
(1S,2R,8S)-14-oxa-7-azatetracyclo[6.6.1.01,11.02,7]pentadeca-9,11-dien-13-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6028 mL | 23.0139 mL | 46.0278 mL | |
| 5 mM | 0.9206 mL | 4.6028 mL | 9.2056 mL | |
| 10 mM | 0.4603 mL | 2.3014 mL | 4.6028 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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