| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| Other Sizes |
Purity: =99.89%
| Targets |
Allosecurinine does not have a single defined molecular target but exerts its effects through multiple mechanisms. It has been shown to inhibit HIV-1 reverse transcriptase and block herpes simplex virus type 1 replication. It exhibits potent cytotoxicity against K562 leukemia cells. Its antifungal activity has also been documented. Securinega alkaloids, including allosecurinine, are known for stimulating the central nervous system (CNS).
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| ln Vitro |
Suggested by Phytophthora plants, leaf base alkaloids are a class of natural products with significant biological activities that include stimulating the central nervous system (CNS) and having antibacterial, anti-tumor, and anti-malarial properties [1].
In vitro, allosecurinine demonstrates significant cytotoxicity against human cancer cells. It inhibits the proliferation of cancer cells by inducing apoptosis and inhibiting cell cycle progression. The compound shows potent cytotoxicity against K562 leukemia cells. It also demonstrates antiviral activity against HIV and herpes simplex virus type 1 (HSV-1) in vitro. Its antifungal activity has been confirmed. |
| ln Vivo |
In vivo, allosecurinine has been studied for its potential antitumor, antiviral, and anti-inflammatory effects. It exhibits significant bioactivity against various cancer cell lines. Its ability to stimulate the central nervous system suggests potential neurological effects. Further in vivo studies are needed to fully characterize its therapeutic potential and pharmacokinetic properties.
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| Enzyme Assay |
In vitro cytotoxicity assays for allosecurinine are performed using various cancer cell lines, including K562 leukemia cells. Cells are treated with allosecurinine at various concentrations for defined time periods. Cell viability is assessed using MTT or CellTiter-Glo assays. Apoptosis is measured by Annexin V/PI staining or caspase-3 activity assays. Cell cycle analysis is performed by flow cytometry to evaluate cell cycle arrest.
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| Cell Assay |
In vitro antiviral assays are conducted using HIV-1-infected cells or HSV-1-infected cells. Cells are treated with allosecurinine at various concentrations, and viral replication is measured by quantifying viral RNA, p24 antigen (for HIV), or plaque reduction assays (for HSV-1). The compound's ability to inhibit HIV-1 reverse transcriptase is measured using enzymatic assays. Anti-inflammatory activity is assessed by measuring cytokine production in immune cells.
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| Animal Protocol |
In vivo animal experiments with allosecurinine are limited. The compound is a natural product with potential therapeutic applications. Further in vivo studies are needed to evaluate its efficacy in cancer, viral infection, and inflammation models. Animals would be administered allosecurinine via injection or oral gavage, and endpoints would include tumor growth inhibition, viral load reduction, and inflammatory marker measurement.
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| ADME/Pharmacokinetics |
Allosecurinine has a molecular formula of C13H15NO2 and a molecular weight of 217.26 g/mol. It is a natural alkaloid compound. The compound is typically stored at -20°C for long-term stability and should be protected from light and moisture. It is supplied with ≥95% purity. It appears as a solid powder. It is soluble in appropriate organic solvents.
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| Toxicity/Toxicokinetics |
As a research chemical with potent biological activity, allosecurinine is not intended for human use. Its toxicological profile is not fully characterized. Standard laboratory safety precautions should be followed when handling it. Its effects on the central nervous system suggest potential neurological toxicity at high doses. Comprehensive toxicology studies are needed for therapeutic development.
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| References | |
| Additional Infomation |
Verosecurinine belongs to the indoleazine class of compounds. It has been reported to exist in Flueggea suffruticosa and Phyllanthus niruri, and relevant data are available. See also: selenocurinine (note moved to); isoculinine (note moved to); verosoisocurinine (note moved to)... See more...
Allosecurinine is also known as Phyllochrysine. It is a Securinega alkaloid isolated from plants of the Phyllanthaceae family. The compound has important biological activities, including stimulating the central nervous system (CNS) and possessing antibacterial, antitumor, and antimalarial properties. It is used in research on cancer, viral infections, and fungal diseases. It is supplied as a research-grade compound. |
| Molecular Formula |
C13H15NO2
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|---|---|
| Molecular Weight |
217.26400
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| Exact Mass |
217.11
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| CAS # |
884-68-4
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| Related CAS # |
(+)-Viroallosecurinine;1857-30-3
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| PubChem CID |
267769
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.30 g/cm3
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| Boiling Point |
459.0±45.0℃ at 760 mmHg
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| Melting Point |
136-138℃
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| LogP |
1.342
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
16
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| Complexity |
426
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CCN2[C@@H](C1)[C@]34C[C@H]2C=CC3=CC(=O)O4
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| InChi Key |
SWZMSZQQJRKFBP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H15NO2/c15-12-7-9-4-5-10-8-13(9,16-12)11-3-1-2-6-14(10)11/h4-5,7,10-11H,1-3,6,8H2
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| Chemical Name |
14-oxa-7-azatetracyclo[6.6.1.01,11.02,7]pentadeca-9,11-dien-13-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~230.14 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (9.57 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (5.75 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6028 mL | 23.0139 mL | 46.0278 mL | |
| 5 mM | 0.9206 mL | 4.6028 mL | 9.2056 mL | |
| 10 mM | 0.4603 mL | 2.3014 mL | 4.6028 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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