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| Targets |
TES sodium does not have a specific pharmacological target as it is a biological buffer. Its function is to maintain a stable pH in biological and biochemical systems. As a zwitterionic buffer, it can act as both a proton donor and acceptor, resisting changes in pH when acids or bases are added. Its pKa of 7.55 makes it particularly useful for applications requiring pH control in the physiological range. TES sodium is compatible with most biological systems and does not interfere with enzymatic reactions or cell growth.
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| ln Vitro |
In vitro, TES sodium is used as a buffering agent in various biochemical and cell biology applications. It is commonly used in cell culture media to maintain pH for cell growth. In enzyme activity assays, TES sodium provides stable pH conditions for optimal enzyme function. The buffer is suitable for epidermal cell growth at pH 7.4-7.9 where additional buffering capacity is required. It is also useful in cell culture media formulations. TES sodium has been found to be beneficial in studies of succinate oxidation.
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| ln Vivo |
TES sodium is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a laboratory buffer for in vitro experiments and biopharmaceutical formulations. The compound is used in cell culture media for maintaining pH in cell culture applications. It is not administered to animals for therapeutic purposes but is used in the preparation of solutions for biological experiments. Its role is limited to maintaining pH homeostasis in experimental systems.
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| Enzyme Assay |
In vitro experiments using TES sodium involve preparing buffer solutions at the desired pH (range 6.8-8.2) by dissolving the appropriate amount of TES sodium in deionized water. The buffer is typically used at concentrations of 10-50 mM. For cell culture applications, TES sodium is added to culture media at 10-25 mM to maintain pH. For enzyme assays, TES buffer is used to prepare substrate solutions and reaction mixtures. The buffer is soluble in water at 200 mg/mL and in DMSO at 60 mg/mL. The pH of the buffer solution can be adjusted with acid or base as needed.
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| Cell Assay |
In vitro cell-based assays using TES sodium involve its incorporation into cell culture media to maintain physiological pH. The compound is dissolved in cell culture medium at concentrations of 10-25 mM. Cells are cultured in TES-buffered media under standard conditions. The buffer is compatible with most cell types and does not affect cell viability at standard concentrations. For studies requiring precise pH control in the range of 6.8-8.2, TES sodium provides effective buffering. The buffer is suitable for epidermal cell growth at pH 7.4-7.9.
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| Animal Protocol |
In vivo animal experiments using TES sodium are not typical as the compound is a research buffer rather than a therapeutic agent. However, TES-buffered solutions may be used in animal studies for the administration of other compounds where pH control is critical. When used in animal studies, TES sodium would be formulated as a buffered saline solution for injection or oral administration. The compound's primary role is to maintain pH stability in administered solutions rather than to exert any pharmacological effect.
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| ADME/Pharmacokinetics |
TES sodium has a molecular weight of 252.24 g/mol and the formula C6H15NNaO6S. The compound has a pKa of 7.55 at 25°C and an effective pH range of 6.8 to 8.2. It is soluble in water at 200 mg/mL and in DMSO at 60 mg/mL. For storage, the powder is kept at -20°C for up to 3 years. TES sodium is a zwitterionic buffer that is compatible with most biological systems. The buffer is stable under normal storage conditions.
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| Toxicity/Toxicokinetics |
TES sodium is generally considered to have low toxicity as a biological buffer. The compound is not intended for human or veterinary use and is classified for research purposes only. Safety data sheets indicate that standard laboratory handling precautions should be followed, including the use of appropriate personal protective equipment. The compound is stable under normal storage conditions. Acute toxicity data are limited, but as a zwitterionic buffer, it is not expected to be highly toxic. In cell culture applications, TES sodium is used at concentrations that are compatible with cell viability and growth.
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| Additional Infomation |
TES sodium (CAS 70331-82-7) is a zwitterionic biological buffer belonging to the Good's buffer family. It is also known as N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid sodium salt. The compound has the molecular formula C6H15NNaO6S and a molecular weight of 252.24 g/mol. TES sodium has a pKa of 7.55 at 25°C and an effective pH range of 6.8 to 8.2. It is commonly used in cell culture and enzyme studies. The buffer is suitable for epidermal cell growth at pH 7.4-7.9 and has been found to be beneficial in studies of succinate oxidation. TES sodium is intended for research use only.
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| Molecular Formula |
C6H14NNAO6S
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| Molecular Weight |
251.2332
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| Exact Mass |
251.043
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| CAS # |
70331-82-7
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| Related CAS # |
TES;7365-44-8
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| PubChem CID |
23702152
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
15
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| Complexity |
239
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C([H])([H])C([H])([H])N([H])C(C([H])([H])O[H])(C([H])([H])O[H])C([H])([H])O[H])(=O)(=O)[O-].[Na+]
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| InChi Key |
GUXMFAHOYNRHBI-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C6H15NO6S.Na/c8-3-6(4-9,5-10)7-1-2-14(11,12)13;/h7-10H,1-5H2,(H,11,12,13);/q;+1/p-1
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| Chemical Name |
sodium;2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~995.10 mM)
DMSO : ~83.33 mg/mL (~331.69 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9804 mL | 19.9021 mL | 39.8042 mL | |
| 5 mM | 0.7961 mL | 3.9804 mL | 7.9608 mL | |
| 10 mM | 0.3980 mL | 1.9902 mL | 3.9804 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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