| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
TES free acid is a buffering agent and does not have a specific pharmacological target. It is used as a biochemical reagent to maintain stable pH in biological systems. It has been reported to form complexes with DNA and affect restriction enzyme kinetics. It is a structural analog to Tris buffer.
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|---|---|
| ln Vitro |
TES is used as a buffering agent in a range of protein assays. It has been used in TES buffer to determine the anti-collagenase activity of Marula stem extracts. It is used in cell culture media to maintain physiological pH. TES forms complexes with DNA and affects restriction enzyme kinetics.
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| ln Vivo |
TES is used as a biological buffer in various in vitro and in vivo applications. It is used in cell culture media to maintain stable pH. It has been used in animal studies as a buffer in various formulations. However, TES itself does not have direct pharmacological activity.
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| Enzyme Assay |
TES is characterized in cell-free systems for its buffering capacity and pKa. The pH of TES buffer solutions is measured using a pH meter at different temperatures to determine the pKa. The compound's ability to maintain pH in the presence of acids or bases is assessed using titration experiments. Its compatibility with various biochemical assays is evaluated.
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| Cell Assay |
TES is used in cell-based assays as a component of cell culture media and buffers. Cells are cultured in media containing TES to maintain stable pH during experiments. The buffer's effects on cell viability, growth, and experimental outcomes are evaluated. TES is particularly useful in cultures requiring divalent metal ions.
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| Animal Protocol |
TES is used in animal studies as a component of buffers for various formulations. It has been used in TES buffer for the determination of anti-collagenase activity. However, TES itself is not a drug and has no direct pharmacological activity in vivo.
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| ADME/Pharmacokinetics |
TES free acid has a molecular weight of 229.25 and a molecular formula of C6H15NO6S. It has a CAS number of 7365-44-8. The compound is a white to off-white solid powder. It is soluble in water at ~125 mg/mL. It has a LogP of -2.62. It should be stored as a powder at -20°C.
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| Toxicity/Toxicokinetics |
TES free acid is generally considered safe as a laboratory reagent. No significant toxicity is associated with its use as a buffer. The compound is for research use only and is not intended for human therapeutic use.
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| References | |
| Additional Infomation |
N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid is a Goodyear buffer with a pKa of 7.5 at 20°C. It belongs to the ethanolamine, aminosulfonic acid, and triethanesulfonic acid classes. Its function is related to taurine. It is a tautomer of N-tris(hydroxymethyl)methyl-2-aminoethanesulfonate.
TES free acid is also known as N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid. It is a Good's buffer. It has a PubChem CID of 81831. The compound is not approved for clinical use. |
| Molecular Formula |
C6H15NO6S
|
|---|---|
| Molecular Weight |
229.2514
|
| Exact Mass |
229.062
|
| CAS # |
7365-44-8
|
| Related CAS # |
TES sodium;70331-82-7;TES-d15;1219794-63-4
|
| PubChem CID |
81831
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| Appearance |
White to off-white solid powder
|
| Density |
1.6±0.1 g/cm3
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| Melting Point |
~225 °C (dec.)
|
| Index of Refraction |
1.571
|
| LogP |
-2.62
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
14
|
| Complexity |
233
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
S(C([H])([H])C([H])([H])N([H])C(C([H])([H])O[H])(C([H])([H])O[H])C([H])([H])O[H])(=O)(=O)O[H]
|
| InChi Key |
JOCBASBOOFNAJA-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C6H15NO6S/c8-3-6(4-9,5-10)7-1-2-14(11,12)13/h7-10H,1-5H2,(H,11,12,13)
|
| Chemical Name |
2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~545.26 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (436.21 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3621 mL | 21.8103 mL | 43.6205 mL | |
| 5 mM | 0.8724 mL | 4.3621 mL | 8.7241 mL | |
| 10 mM | 0.4362 mL | 2.1810 mL | 4.3621 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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