| Size | Price | Stock | Qty |
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| 50g |
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| 100g |
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| 200g |
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| Other Sizes |
| Targets |
Sudan Black B does not have a specific molecular target but functions as a lipophilic dye that stains neutral lipids and phospholipids in biological samples. The dye binds to lipid droplets, cell membranes, and myelin sheaths, allowing for the visualization of lipid-rich structures in tissues and cells. Its staining mechanism is based on its high affinity for hydrophobic lipid environments, where it partitions and becomes visible due to its intense black color. This makes it a valuable tool for identifying lipid deposits and differentiating between tissue components in histological studies.
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| ln Vitro |
Sudan Black B can be utilized in conjunction with SA-β-gal for researching aging because it is also appropriate for freezing materials. Sudan Black B is a very lipophilic reagent with a strong affinity for lipofuscin's lipid compartment. The B-lipofuscin response to Sudan black is positive. Blue-black intracellular particles can be observed under a light microscope in frozen tissues and cells, and brown-black particles in FFPE tissues.
In vitro, Sudan Black B is used as a histological stain for the detection of lipids in tissue sections, cell smears, and frozen sections. It is particularly useful for staining lipids in cell cultures and tissue samples. The dye is also used to stain older blood or bone marrow smears, though the stain slightly fades over time. Its staining intensity is concentration-dependent, with optimal staining typically achieved using a saturated solution in 70% ethanol. Sudan Black B is considered a sensitive and specific reagent for lipid staining. It is often used in studies of lipid metabolism, fat storage, and neurobiology. |
| ln Vivo |
In vivo, Sudan Black B is not used as a therapeutic agent but as a research tool in histology and cell biology. It is used in ex vivo studies on tissue samples to identify lipid deposits and study lipid metabolism. The compound is not administered to live animals for therapeutic purposes. Its in vivo applications are limited to post-mortem tissue staining and histological analysis. The compound is for research use only and is not approved for human therapeutic applications.
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| Enzyme Assay |
The in vitro lipid staining protocol for Sudan Black B typically involves fixing tissue sections or cell smears, followed by incubation with a saturated solution of Sudan Black B in 70% ethanol or other suitable solvent. The staining time varies from 5 to 30 minutes depending on the sample type. After staining, the samples are rinsed with 70% ethanol to remove excess dye and counterstained with other stains if needed. The stained lipids appear black or dark blue under bright-field microscopy. For specificity testing, lipid extraction controls (e.g., using organic solvents) are performed to confirm that the staining is lipid-specific. Positive controls (e.g., known lipid-rich tissues) and negative controls (lipid-extracted samples) are included in each staining run.
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| Cell Assay |
For in vitro cellular assays, cells or tissue sections are stained with Sudan Black B using standard histological protocols. Cells are fixed with formalin or other fixatives, stained with Sudan Black B solution, and examined by light microscopy. Lipid content is quantified by image analysis or by measuring the absorbance of extracted dye. Cell viability is not typically assessed with Sudan Black B, as it is a staining reagent rather than a pharmacologically active compound. For lipid metabolism studies, cells are treated with compounds that modulate lipid accumulation (e.g., fatty acids, inhibitors) and then stained with Sudan Black B to assess changes in lipid content. All experiments include appropriate controls (untreated cells, lipid-extracted samples) and are performed in triplicate.
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| Animal Protocol |
For in vivo studies, Sudan Black B is not typically administered to animals as a therapeutic agent. It is used in ex vivo studies on tissue samples obtained from animals. Tissues are harvested, fixed, sectioned, and stained with Sudan Black B for histological analysis. The staining is used to identify lipid deposits, assess lipid metabolism, and study neurobiology. All animal procedures should be conducted in accordance with institutional guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Sudan Black B are not relevant, as it is a histological stain rather than a therapeutic agent. The compound has a molecular weight of 456.54 and is a lipophilic dye. It is used in vitro and ex vivo for staining applications. The compound should be stored in a cool, dry place protected from light. Detailed PK data are not available in publicly accessible literature.
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| Toxicity/Toxicokinetics |
The toxicology of Sudan Black B has been partially characterized. As a histological dye, it is not intended for human consumption or therapeutic use. In handling, the compound may cause staining of skin and clothing. Inhalation of dust may cause respiratory irritation. The compound is not genotoxic in standard in vitro assays. Sudan Black B should be handled with appropriate laboratory safety precautions, including the use of personal protective equipment. The compound is for research use only and is not approved for human therapeutic use.
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| References | |
| Additional Infomation |
Sudan Black B belongs to the pyrimidine class of compounds. Its chemical name is 2,2-dimethyl-2,3-dihydro-1H-pyrimidine, with a [4-(phenyldiazonyl)naphth-1-yl]diazonyl substituent at the 6-position. It is a lipid-soluble dye, mainly used for staining triglycerides in frozen sections and protein-bound lipids in paraffin sections. It is a histological dye. Sudan Black B belongs to the azobenzene class of compounds, is a bis(azo) compound, and also belongs to the pyrimidine class of compounds.
Sudan Black B is a basic black dye used for staining lipids in histology and cell biology. It is considered a sensitive and specific reagent for lipid staining. The compound is not approved for human use and is intended for research purposes only. It is available as a high-purity research reagent for laboratory use. Its lipophilic staining properties make it a valuable tool for studying lipid metabolism, fat storage, neurobiology, and tissue differentiation. |
| Molecular Formula |
C29H24N6
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| Molecular Weight |
456.54
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| Exact Mass |
456.206
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| CAS # |
4197-25-5
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| PubChem CID |
61336
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| Appearance |
Brown to black solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
726.0±60.0 °C at 760 mmHg
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| Melting Point |
120-124 °C(lit.)
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| Flash Point |
392.9±32.9 °C
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| Vapour Pressure |
0.0±2.4 mmHg at 25°C
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| Index of Refraction |
1.702
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| LogP |
7.51
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
35
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| Complexity |
773
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YCUVUDODLRLVIC-VPHDGDOJSA-N
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| InChi Code |
InChI=1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
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| Chemical Name |
2,3-Dihydro-2,2-dimethyl-6-((1-naphthyl-4-(phenylazo))azo)-1H-perimidine
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| Synonyms |
CI-26150 CI26150 CI26150 Sudan Black B
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~1 mg/mL (~2.19 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1904 mL | 10.9519 mL | 21.9039 mL | |
| 5 mM | 0.4381 mL | 2.1904 mL | 4.3808 mL | |
| 10 mM | 0.2190 mL | 1.0952 mL | 2.1904 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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