| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 100mg |
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| ln Vitro |
- Secoisolariciresinol showed significant DPPH radical scavenging activity with an IC50 value of 28.4 ± 1.2 μM, which was comparable to the positive control (ascorbic acid, IC50 = 22.1 ± 0.8 μM) [1]
- In the ABTS cation radical scavenging assay, Secoisolariciresinol exhibited dose-dependent activity, with a scavenging rate of 89.6 ± 2.3% at 100 μM, and an IC50 value of 31.7 ± 1.5 μM [1] - The compound displayed moderate ferric reducing antioxidant power (FRAP), with a reducing capacity of 1.24 ± 0.08 mmol Fe²⁺/g at 100 μM [1] - Secoisolariciresinol showed weak ferrous ion chelating activity, with a chelating rate of 32.8 ± 2.1% at 200 μM [1] - Gastrointestinal stability assay revealed that Secoisolariciresinol was stable in simulated gastric fluid (pH 1.2) with a residual rate of 92.3 ± 3.1% after 2 hours of incubation. In simulated intestinal fluid (pH 6.8), the residual rate was 87.6 ± 2.8% after 4 hours of incubation [1] |
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| ln Vivo |
- In mice treated with Secoisolariciresinol (oral administration at 50, 100, and 200 mg/kg body weight) for 7 consecutive days, the serum superoxide dismutase (SOD) activity was significantly increased by 28.5%, 42.3%, and 56.7%, respectively, compared to the control group [1]
- The hepatic catalase (CAT) activity in treated mice was elevated by 24.6% (50 mg/kg), 38.9% (100 mg/kg), and 51.2% (200 mg/kg) compared to the control, with a dose-dependent trend [1] - Secoisolariciresinol significantly reduced the malondialdehyde (MDA) content in mouse serum and liver. At 200 mg/kg, serum MDA was decreased by 48.9% and hepatic MDA by 53.4% compared to the control group [1] - The glutathione (GSH) level in mouse liver was increased by 31.2% (50 mg/kg), 45.8% (100 mg/kg), and 62.3% (200 mg/kg) after administration of Secoisolariciresinol [1] |
| Enzyme Assay |
- DPPH radical scavenging assay: A series of dilutions of Secoisolariciresinol (10–100 μM) were mixed with DPPH radical solution. The mixture was incubated in the dark at room temperature for 30 minutes, and the absorbance was measured at 517 nm. The scavenging rate was calculated, and the IC50 value was determined by regression analysis [1]
- ABTS radical cation scavenging assay: ABTS radical cation was generated by reacting ABTS with potassium persulfate. Secoisolariciresinol solutions (10–100 μM) were added to the radical solution, incubated for 15 minutes at room temperature, and absorbance was measured at 734 nm. The scavenging rate and IC50 value were calculated [1] - FRAP assay: Secoisolariciresinol samples (20–100 μM) were mixed with FRAP reagent (containing ferric tripyridyltriazine complex) and incubated at 37°C for 30 minutes. The absorbance was measured at 593 nm, and the reducing capacity was calculated using a ferrous sulfate standard curve [1] - Ferrous ion chelating assay: Secoisolariciresinol solutions (50–200 μM) were mixed with ferrous chloride solution and ferrozine reagent. After incubation at room temperature for 10 minutes, the absorbance was measured at 562 nm, and the chelating rate was calculated [1] |
| Animal Protocol |
- In vivo antioxidant activity assay: Male ICR mice (20–25 g) were randomly divided into control group (distilled water) and Secoisolariciresinol treatment groups (50, 100, 200 mg/kg body weight). The compound was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na) and administered orally once daily for 7 consecutive days. On the 8th day, mice were anesthetized, and serum and liver tissues were collected for the determination of SOD, CAT, MDA, and GSH levels [1]
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| ADME/Pharmacokinetics |
Gastrointestinal stability: Secoisolariciresinol was incubated with gentle shaking in simulated gastric fluid (pH 1.2, containing pepsin) at 37°C. Samples were taken at 0, 0.5, 1 and 2 hours, and the residues of the compound were quantitatively analyzed by high performance liquid chromatography (HPLC). For simulated intestinal fluid (pH 6.8, containing pancreatin), incubation was performed at 0, 1, 2, 3 and 4 hours, and the residues of Secoisolariciresinol were determined by high performance liquid chromatography (HPLC) [1]
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| References | |
| Additional Infomation |
(-)-secoisolariciresinol is the enantiomer of secoisolariciresinol, with the (-)-(2R,3R)- configuration. It possesses antidepressant, phytometrogenic, and phytoestrogenic activities. It is the enantiomer of (+)-secoisolariciresinol. Secoisolariciresinol has been used in clinical trials for breast cancer prevention. It has been reported to be found in tea (Camellia sinensis) and radish (Raphanus sativus), as well as other organisms with relevant data.
- Secoisolariciresinol is a lignan compound isolated from the wood extract of Abies alba [1] - Secoisolariciresinol has antioxidant mechanisms including scavenging free radicals (DPPH, ABTS), enhancing the activity of endogenous antioxidant enzymes (SOD, CAT), increasing GSH levels, and inhibiting lipid peroxidation (reducing MDA production) [1] - Secoisolariciresinol has good gastrointestinal stability, indicating its potential for oral administration and improving in vivo bioavailability [1] - Secoisolariciresinol helps to improve the overall antioxidant activity of Abies alba wood extract, supporting its potential application as a natural antioxidant [1] |
| Molecular Formula |
C20H26O6
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|---|---|
| Molecular Weight |
362.4168
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| Exact Mass |
362.172
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| CAS # |
29388-59-8
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| Related CAS # |
Secoisolariciresinol diglucoside;257930-74-8;(R,R)-Secoisolariciresinol diglucoside;158932-33-3;Secoisolariciresinol Monoglucoside;63320-67-2
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| PubChem CID |
65373
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
609.1±55.0 °C at 760 mmHg
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| Melting Point |
112-114ºC
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| Flash Point |
322.1±31.5 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
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| Index of Refraction |
1.599
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| LogP |
0.98
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
26
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| Complexity |
357
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| Defined Atom Stereocenter Count |
2
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| SMILES |
COC1=C(C=CC(=C1)C[C@@H](CO)[C@@H](CC2=CC(=C(C=C2)O)OC)CO)O
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| InChi Key |
PUETUDUXMCLALY-HOTGVXAUSA-N
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| InChi Code |
InChI=1S/C20H26O6/c1-25-19-9-13(3-5-17(19)23)7-15(11-21)16(12-22)8-14-4-6-18(24)20(10-14)26-2/h3-6,9-10,15-16,21-24H,7-8,11-12H2,1-2H3/t15-,16-/m0/s1
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| Chemical Name |
(2R,3R)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~275.92 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | |
| 5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | |
| 10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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