| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Xanthine oxidase (XO) – Prudomestin showed an IC50 value of 6.73 μM against xanthine oxidase [2].
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|---|---|
| ln Vitro |
Prudomestin demonstrated potent xanthine oxidase (XO) inhibitory activity with an IC50 value of 6.73 μM, which was comparable to the positive control allopurinol (IC50 = 3.38 μM) [2].
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| Enzyme Assay |
The xanthine oxidase (XO) inhibition assay was performed in a 96-well microplate format. Test compounds (including Prudomestin) and allopurinol (positive control) were dissolved in DMSO and then diluted with 0.1 M sodium phosphate buffer (pH 7.8) to achieve working concentrations ranging from 0.01 to 100 μM. For the reaction mixture, 55 μL of the test compound working solution, 40 μL of 0.15 mM xanthine (substrate), and 5.0 μL of xanthine oxidase (0.2 U/mL) were added per well. The mixture was pre-incubated for 20 minutes, and the reaction was initiated by adding the enzyme. The rate of uric acid production was monitored by measuring absorbance at 290 nm every 60 seconds for 10 minutes using a UV-Vis spectrophotometer. The percent inhibition was calculated using the formula: % inhibition = (Abs_control - Abs_sample) / Abs_control × 100. The IC50 values were determined from dose-response curves using non-linear regression analysis according to the Hill equation. Baseline correction was performed by subtracting the optical density of individual sample blanks (without substrate and enzyme) at the corresponding measurement time points [2].
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| References |
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| Additional Infomation |
Prudomestin is a hydroxyflavonoid. It has been reported to be found in European plum (Prunus domestica) and Sichuan pepper (Zanthoxylum acanthopodium), and relevant data are available for reference.
Prudomestin was isolated from the hexane fraction of Zanthoxylum armatum fruits. Its content in the plant material was 0.013% based on dry weight [1]. In a separate study, Prudomestin (ZA-6) was not detected (ND) in the fruits from different geographical locations (Garhwal, India; Lucknow, India; Surkhet, Nepal) when extracted with various solvents and techniques, suggesting it may be an artifact formed during column chromatography rather than a genuine natural product [2]. The compound has been evaluated for xanthine oxidase inhibitory activity, which supports the traditional use of Z. armatum in gout management [2]. No other additional information (e.g., clinical status, FDA warnings) was provided [1][2]. |
| Molecular Formula |
C17H14O7
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|---|---|
| Molecular Weight |
330.28886
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| Exact Mass |
330.073
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| CAS # |
3443-28-5
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| PubChem CID |
10404353
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| Appearance |
Light yellow to yellow solid
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
568.9±50.0 °C at 760 mmHg
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| Melting Point |
209 - 210 °C
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| Flash Point |
212.2±23.6 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
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| Index of Refraction |
1.681
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| LogP |
2.01
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
24
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| Complexity |
509
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1C=CC(C2OC3C(=C(C=C(O)C=3C(=O)C=2O)O)OC)=CC=1
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| InChi Key |
HLSIOUXODPWHFI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O7/c1-22-9-5-3-8(4-6-9)15-14(21)13(20)12-10(18)7-11(19)16(23-2)17(12)24-15/h3-7,18-19,21H,1-2H3
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| Chemical Name |
3,5,7-trihydroxy-8-methoxy-2-(4-methoxyphenyl)chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0276 mL | 15.1382 mL | 30.2764 mL | |
| 5 mM | 0.6055 mL | 3.0276 mL | 6.0553 mL | |
| 10 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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