| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
As an inorganic salt, TSP does not have a specific biological target. It provides sodium and phosphate ions and is used to adjust pH. At high concentrations, it precipitates calcium and magnesium, which can inhibit enzyme activities that depend on these cations.
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|---|---|
| ln Vitro |
In vitro, TSP (1-50 mM) is used as a buffering agent (phosphate buffer). In enzyme assays, it provides phosphate ions and maintains pH stability. High concentrations may inhibit DNA polymerase and restriction enzymes due to high ionic strength, but it is not a direct enzyme inhibitor.
|
| ln Vivo |
No direct in vivo activity. It is used as a food additive but is not administered as a drug. In laxative formulations, TSP can induce osmotic diarrhea at doses >5 g.
|
| Enzyme Assay |
Not applicable; no specific binding assays. For quality control, purity is determined by titration. Solubility in water is high (>100 g/L). pH of a 1% solution should be 12-13.
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| Cell Assay |
In cell culture, TSP is not recommended due to its high pH and caustic nature; it can be used to prepare phosphate buffer (by combining with NaH2PO4). For cytotoxicity, concentrations >10 mM may cause osmotic stress and cell death.
|
| Animal Protocol |
For in vivo studies, TSP (0.1-1 g/kg) can be administered orally to rodents as a laxative or to induce phosphate loading. IV administration is dangerous due to caustic effects and severe tissue damage. It is rapidly excreted in urine.
|
| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
Sodium phosphate has low absorption and relatively low toxicity. /SRP: Systemic / /Orthophosphate/ is absorbed from the gastrointestinal tract and secreted in small amounts. The transport of phosphate from the intestinal lumen is an active, energy-dependent process influenced by multiple factors. …Vitamin D stimulates phosphate absorption; this effect has been reported to precede its effect on calcium ion transport. In adults, approximately two-thirds of ingested phosphate is absorbed, and almost all absorbed phosphate is excreted in the urine. In growing children, the phosphate balance is positive. Children have higher plasma phosphate concentrations than adults. This “hyperphosphatemia” reduces the affinity of hemoglobin for oxygen and is considered an explanation for physiological “anemia” in children. /Phosphate/ The compound is highly soluble in water. Orally administered phosphate is absorbed in the small intestine, distributed in the extracellular fluid, and excreted by the kidneys (t1/2 6-12 hours). It is not metabolized. |
| Toxicity/Toxicokinetics |
Toxicity Summary
The CIR expert panel concluded that the following 31 ingredients are safe for use in cosmetics at current methods and concentrations, provided that the formulation explicitly states they are non-irritating… trisodium phosphate… Safe for use in cosmetics under specific conditions. Interactions In rabbits fed an atherosclerosis-inducing diet, concurrent administration of trisodium phosphate (50 mg/kg diet) suppressed the elevation of total plasma lipids and total cholesterol in the untreated control group during the first month. These levels increased slightly in the following month, but remained lower than in the control group. The mechanism of action is believed to be related to the metabolism of inorganic phosphates. Non-Human Toxicity Values Oral LD50 in rats: 4.8 mg/kg; Oral LD50 in rats: >2000 mg/kg; Inhalation LD50 in rats: ≥2.16 mg/L/1 hr; Skin LD50 in rabbits: ≥2 mg/kg body weight TSP is corrosive and causes severe skin and eye burns. Inhalation of dust causes respiratory tract irritation. Oral LD₅0 in rats is approximately 7400 mg/kg. Use full PPE (gloves, goggles, face shield, lab coat). Avoid contact with acids (releases heat and toxic gases). |
| Additional Infomation |
Sodium tribasic phosphate is a colorless to white crystalline solid, non-flammable. Sodium tribasic phosphate is a type of sodium phosphate. The CIR panel concluded that the following 31 ingredients are safe at current cosmetic application methods and concentrations (as described in this safety assessment), provided the formulation is non-irritating…sodium tribasic phosphate…
Trisodium phosphate is a food additive and industrial chemical; not an FDA-approved drug. It is used as a laboratory reagent (buffer, cleaning agent). Not for human therapeutic use. For research use only. |
| Molecular Formula |
NA3PO4
|
|---|---|
| Molecular Weight |
163.94
|
| Exact Mass |
163.923
|
| CAS # |
7601-54-9
|
| Related CAS # |
10101-89-0 (dodecahydrate)
; 7632-05-5 (Parent)
|
| PubChem CID |
24243
|
| Appearance |
White to off-white solid powder
|
| Density |
2.536 g/cm3 (17.5 C)
|
| Boiling Point |
158ºC at 760 mmHg
|
| Melting Point |
170.06 °F (USCG, 1999)
; 170 °F
; 1583 °C
|
| LogP |
0.386
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
8
|
| Complexity |
36.8
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
P(=O)([O-])([O-])[O-].[Na+].[Na+].[Na+]
|
| InChi Key |
RYFMWSXOAZQYPI-UHFFFAOYSA-K
|
| InChi Code |
InChI=1S/3Na.H3O4P/c;;;1-5(2,3)4/h;;;(H3,1,2,3,4)/q3*+1;/p-3
|
| Chemical Name |
trisodium;phosphate
|
| Synonyms |
Trisodium phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~200 mg/mL (~1219.96 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.0998 mL | 30.4990 mL | 60.9979 mL | |
| 5 mM | 1.2200 mL | 6.0998 mL | 12.1996 mL | |
| 10 mM | 0.6100 mL | 3.0499 mL | 6.0998 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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