| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
5-Propargylamino-3'-azidomethyl-dCTP TEA has no biological target. It is a synthetic substrate for DNA polymerases. The azidomethyl group at the 3' position acts as a reversible terminator, blocking further nucleotide addition after incorporation. This property is essential for next-generation sequencing (SBS). The propargylamino group provides a chemical handle (an alkyne) for attaching fluorophores or biotin via click chemistry after DNA synthesis is complete.
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| ln Vitro |
The "activity" is the ability to serve as a substrate for DNA polymerases and to undergo click chemistry. In an in vitro polymerization assay, it is incorporated with high efficiency (similar to natural dCTP). The 3'-azidomethyl group can be cleaved chemically after each cycle to allow chain elongation. The propargylamine group is used in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) to attach azide-labeled molecules (e.g., azide-Cy3). The TEA salt improves solubility and removes inhibitors for polymerases.
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| ln Vivo |
Specific in vivo activity data is not applicable. This compound is not administered to animals. It is used in cell-free molecular biology applications. However, modified nucleotides like this can be used in "Edu" or "BrdU" type assays if they can be delivered into cells via permeabilization, but it is not standard. Its primary use is in in vitro diagnostics and research assays.
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| Enzyme Assay |
Not applicable. This compound is a substrate for polymerases, not an inhibitor. It is not used in standard enzyme inhibition or receptor binding assays. Its incorporation can be measured in a primer extension assay. Procedure: Design a synthetic DNA template. Prepare a master mix with 50 mM Tris-Cl, pH 8.0, 10 mM MgCl2, 1 mM DTT, 0.5 uM DNA template, 0.5 uM primer, 100 uM dATP, dGTP, and dTTP. Add 100 uM of the modified dCTP (5-Propargylamino-3'-azidomethyl-dCTP) to the reaction mix. Add DNA polymerase (e.g., 9degN exo-). Run PCR for 15 cycles. Analyze by PAGE. A visible band at the correct length indicates successful incorporation.
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| Cell Assay |
Not applicable. The compound itself is not applied directly to live cells because it is a highly polar triphosphate that cannot cross the cell membrane. However, it can be used to label DNA in fixed cells. Procedure: Prepare cytospins of Jurkat cells. Fix cells with 4% PFA, permeabilize with 0.2% Triton X-100. Incubate cells with TdT enzyme (terminal deoxynucleotidyl transferase) and 5-Propargylamino-3'-azidomethyl-dCTP TEA (50 uM) to end-label fragmented DNA (TUNEL). Perform click chemistry: incubate cells with 10 uM CuSO4, 50 uM AF488-azide, and 10 mM sodium ascorbate for 30 min. Wash. The presence of green fluorescence indicates DNA strand breaks (apoptosis).
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| Animal Protocol |
Not applicable. The compound is not used in live animals. However, it can be used to detect apoptosis in tissue sections. Procedure: Prepare 5 um paraffin-embedded sections of mouse liver tissue (e.g., from a drug-induced liver injury model). Deparaffinize, rehydrate, and permeabilize. Treat sections with a TUNEL reaction mixture containing 5-Propargylamino-3'-azidomethyl-dCTP TEA and TdT. Perform a click reaction with an alkyne-fluorophore. Counterstain with DAPI. The number of TUNEL-positive (green) cells in the tissue section is proportional to the level of apoptosis induced by the drug treatment.
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| ADME/Pharmacokinetics |
PK data is not applicable. The compound is a triphosphate nucleotide and cannot cross cell membranes. It is not a systemic drug. For in vitro use, it is supplied as a solution (in water) to avoid freeze-thaw degradation, as triphosphates are sensitive to nucleases and phosphatases. The TEA salt (triethylammonium) is preferred over sodium salts because TEA can be removed more easily during DNA synthesis, leaving a cleaner product.
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| Toxicity/Toxicokinetics |
Specific toxicity data is not provided. The compound is not intended for in vivo use. Standard safety handling for molecular biology reagents applies. Avoid contact with skin and eyes. It should be stored at -20degC or -80degC to maintain stability.
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| References | |
| Additional Infomation |
5-Propargylamino-3'-azidomethyl-dCTP TEA is a reversible terminator nucleotide for next-generation sequencing (NGS). In SBS, each of the four nucleotides (dATP, dGTP, dCTP, dTTP) is present in this modified form, each with a different fluorescent dye attached via a cleavable linker. This allows for "sequencing by synthesis." This specific analog (dCTP) provides the functionality for incorporating and imaging the "C" base. This product is for research use only.
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| Molecular Formula |
C13H20N7O13P3.3.3C6H15N
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|---|---|
| Molecular Weight |
909.19
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| Appearance |
Solid Powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~137.49 mM; with ultrasonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0999 mL | 5.4994 mL | 10.9988 mL | |
| 5 mM | 0.2200 mL | 1.0999 mL | 2.1998 mL | |
| 10 mM | 0.1100 mL | 0.5499 mL | 1.0999 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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