| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Hydrophobic regions and pockets on proteins. ANS does not have a single defined protein target; it binds nonspecifically to hydrophobic surfaces, especially those exposed on unfolded or partially folded proteins.
|
|---|---|
| ln Vitro |
ANS is a hydrophobic fluorescent probe. In aqueous solution, ANS has very low fluorescence quantum yield. Upon binding to hydrophobic protein surfaces, its fluorescence emission increases significantly (excitation ~350‑385 nm, emission ~480‑500 nm). This property makes it useful for detecting protein conformational changes, measuring ligand binding affinities, and monitoring protein folding and aggregation.
|
| ln Vivo |
Not applicable. ANS is used exclusively for in vitro biochemical applications and is not used in vivo as a therapeutic or imaging agent. It is a research tool for studying protein structure and interactions in purified systems or cell lysates.
|
| Enzyme Assay |
A general cell‑free protocol for using ANS as a fluorescent probe: Protein samples (0.1‑1 mg/mL) are prepared in buffer (e.g., 20 mM Tris‑HCl, pH 7.4, 100 mM NaCl). ANS is added to a final concentration of 10‑100 uM from a stock solution in DMSO or methanol. After incubation for 5‑10 minutes at room temperature, the fluorescence is measured at 25degC using a fluorescence spectrophotometer (excitation 350‑385 nm, emission 480‑500 nm). Increased fluorescence indicates binding of ANS to hydrophobic surfaces, often associated with protein unfolding. For competition assays, unlabeled hydrophobic ligands are added to compete with ANS binding.
|
| Cell Assay |
General cellular protocol for ANS is not typically used, as ANS is a cell‑impermeable probe for in vitro studies. For live‑cell studies, more cell‑permeable probes such as Nile Red or other hydrophobic fluorophores are used. ANS is used in cell lysates to assess protein aggregation or denaturation. Cells are lysed in buffer containing protease inhibitors, and the lysate is incubated with ANS (20‑100 uM). Fluorescence is measured to assess the exposure of hydrophobic protein surfaces, indicating protein denaturation or aggregation.
|
| Animal Protocol |
Not applicable. ANS is not used in animal studies. It is an in vitro research reagent. For in vivo fluorescence imaging, other fluorophores such as near‑infrared (NIR) dyes are typically used due to ANS‘s low tissue penetration and high background fluorescence.
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| References |
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| Additional Infomation |
8-Phenylinonaphthalene-1-sulfonic acid is a naphthalenesulfonic acid formed by substituting phenylamino groups at the 8-position of naphthalene-1-sulfonic acid. It can be used as a fluorescent probe. It is an aminonaphthalene and naphthalenesulfonic acid.
It has been reported that 8-phenylylinonaphthalene-1-sulfonic acid exists in humans, and relevant data are available for reference. ANS has a molecular formula of C16H13NO3S and a molecular weight of 299.35 g/mol (free acid). It is also known as 1,8‑ANS, 1‑Anilino‑8‑naphthalenesulfonic acid, and phenylperi acid. The compound is supplied as a dark gray to gray‑green crystalline powder. It is soluble in methanol (50 mg/mL) and chloroform (30 mg/mL). ANS is a classic fluorescent probe for studying protein conformation, first characterized by Weber and Young in 1964. It is not intended for human therapeutic use. |
| Molecular Formula |
C16H13NO3S
|
|---|---|
| Molecular Weight |
299.34
|
| Exact Mass |
299.061
|
| CAS # |
82-76-8
|
| Related CAS # |
1445-19-8 (mono-hydrochloride);18108-68-4 (magnesium [2:1]);28836-03-5 (mono-ammonium)
|
| PubChem CID |
1369
|
| Appearance |
Solid powder
|
| Density |
1.4±0.1 g/cm3
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| Melting Point |
215-217 °C (dec.)(lit.)
|
| Index of Refraction |
1.710
|
| LogP |
2.45
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
21
|
| Complexity |
439
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=S(C1C2C(=CC=CC=2NC2C=CC=CC=2)C=CC=1)(O)=O
|
| InChi Key |
FWEOQOXTVHGIFQ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C16H13NO3S/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13/h1-11,17H,(H,18,19,20)
|
| Chemical Name |
8-anilinonaphthalene-1-sulfonic acid
|
| Synonyms |
8-Anilino-1-naphthalenesulfonic acid; ANSA; 8-Anilino-1-naphthalenesulfonic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~167.0 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (20.8 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (20.8 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3407 mL | 16.7034 mL | 33.4068 mL | |
| 5 mM | 0.6681 mL | 3.3407 mL | 6.6814 mL | |
| 10 mM | 0.3341 mL | 1.6703 mL | 3.3407 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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