Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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ln Vitro |
PTK7/β-catenin-IN-1 (compound 01065; 72 hours) exhibits antiproliferative properties in HCT116, SW480, and MEF with IC50 values of 19.6 µM, 21.3 µM, and 41 µM, respectively[1]. PTK7/β-catenin-IN-1 (25 µM; 24 hours) increased the percentage of HCT116 and SW480 cells in S phase, followed by a decrease in G0/G1 phase, indicating that S phase arrest inhibits cell cycle progression[1]. PTK7/β-catenin-IN-1 (25 µM; 24 hours) resulted in a significant increase in p21 and p27 protein levels[1]. PTK7/β-catenin-IN-1 (25 µM; 24 hours) resulted in a significant decrease in AXIN2 mRNA levels in HCT116 and SW480[1]. Cell Cycle Analysis[1] Cell Line: HCT116 and SW480 cells Concentration: 25 µM Incubation Time: 24 h Result: Induced an obvious cell cycle arrest through distinct mechanisms. Increased the percentage of HCT116 and SW480 cells in S phase with subsequent decrease in G0/G1 phases suggesting S phase arrest that inhibits cell cycle progression.
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Cell Assay |
Western Blot Analysis[1]
Cell Types: HCT116 and SW480 Tested Concentrations: 25 µM Incubation Duration: 24 h Experimental Results: Caused a significant increase in p21 and p27 protein levels. RT-PCR[1] Cell Types: HCT116 and SW480 Tested Concentrations: 25 µM Incubation Duration: 24 h Experimental Results: Caused a significant reduction in AXIN2 mRNA leve. |
References |
Molecular Formula |
C22H16N2O2
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Molecular Weight |
340.37
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Exact Mass |
340.121
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CAS # |
906147-24-8
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PubChem CID |
7160404
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Appearance |
Solid powder
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Density |
1.310±0.06 g/cm3(Predicted)
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LogP |
4.4
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Hydrogen Bond Donor Count |
2
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Hydrogen Bond Acceptor Count |
2
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Rotatable Bond Count |
4
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Heavy Atom Count |
26
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Complexity |
514
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Defined Atom Stereocenter Count |
0
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SMILES |
N1C2=C(C=CC=C2)C(C(=O)C(NC2=CC=CC=C2C2=CC=CC=C2)=O)=C1
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InChi Key |
YEZRIBXANRYCPY-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C22H16N2O2/c25-21(18-14-23-19-12-6-5-11-17(18)19)22(26)24-20-13-7-4-10-16(20)15-8-2-1-3-9-15/h1-14,23H,(H,24,26)
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Chemical Name |
2-(1H-indol-3-yl)-2-oxo-N-(2-phenylphenyl)acetamide
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 60 mg/mL (176.28 mM; with sonication)
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9380 mL | 14.6899 mL | 29.3798 mL | |
5 mM | 0.5876 mL | 2.9380 mL | 5.8760 mL | |
10 mM | 0.2938 mL | 1.4690 mL | 2.9380 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.