| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 1g | |||
| Other Sizes |
Purity: Protein Content=92.2%
| Targets |
Trypsin's primary targets are peptide bonds adjacent to basic amino acids (lysine and arginine) in proteins. It also activates protease-activated receptors (PARs), particularly PAR2 and PAR4, which are involved in cell proliferation, differentiation, and neurogenic inflammation. PAR2 activation promotes wound healing, while PAR4 is involved in inflammatory responses. Trypsin does not target a single receptor but rather cleaves multiple protein substrates.
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| ln Vitro |
In vitro, trypsin is used for protein digestion and structural analysis. It exhibits proteolytic activity that can be measured using synthetic chromogenic substrates such as N-benzoyl-L-arginine ethyl ester (BAEE) or N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA). The enzyme has optimal activity at pH 7–8. Trypsin also shows anti-inflammatory activity and can induce membrane fusion in cells infected with PDCoV through interaction of its S glycoprotein with pAPN.
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| ln Vivo |
In vivo, trypsin has been shown to promote wound healing. It is used clinically in the treatment of various inflammatory conditions, ulcers, trauma, edema, and skin diseases. It has also been used in the treatment of pulmonary emphysema and bronchitis. Trypsin exerts its effects through proteolytic degradation of necrotic tissue and activation of PARs, promoting tissue repair and resolution of inflammation.
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| Enzyme Assay |
In vitro enzyme assays for trypsin activity are performed using synthetic peptide substrates. The enzyme is incubated with a chromogenic or fluorogenic substrate at 25–37°C in a suitable buffer (e.g., Tris-HCl, pH 7.5–8.0). The release of the chromophore or fluorophore is measured continuously using a spectrophotometer or fluorometer. Activity is expressed as units per mg of protein. Inhibition studies with protease inhibitors are used to characterize specificity.
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| Cell Assay |
In vitro cellular assays using trypsin involve the assessment of PAR2 and PAR4 activation. Cells expressing PAR2 or PAR4 (e.g., human airway epithelial cells or fibroblasts) are treated with various concentrations of trypsin. Receptor activation is measured by intracellular calcium mobilization using fluorescent calcium indicators, or by monitoring downstream signaling pathways such as MAPK activation. The effects of trypsin on cell proliferation and migration can also be assessed.
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| Animal Protocol |
In vivo animal studies for trypsin are conducted in wound healing models. Rats or mice are subjected to full-thickness skin wounds, and trypsin is applied topically or administered systemically. Wound closure is monitored over time, and histological analysis is performed to assess granulation tissue formation, re-epithelialization, and collagen deposition. The anti-inflammatory effects of trypsin are evaluated in models of acute or chronic inflammation.
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| ADME/Pharmacokinetics |
Trypsin is not administered systemically as a drug; it is typically used topically or as a reagent. When used therapeutically, its activity is localized to the site of application. The enzyme is stable indefinitely in dry form at room temperature but should be protected from moisture. It is soluble in water and practically insoluble in alcohol or glycerol. Trypsin is inactivated by heat and protease inhibitors.
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| Toxicity/Toxicokinetics |
Trypsin is generally considered safe for topical and research applications. When used clinically, it is well-tolerated with minimal side effects. As a proteolytic enzyme, it may cause irritation if applied to sensitive tissues. Systemic toxicity is low because the enzyme is large and does not absorb significantly through intact skin. Standard safety precautions for handling enzymes apply, including the use of gloves and eye protection.
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| References | |
| Additional Infomation |
Trypsin is a proteolytic enzyme widely used in biochemical research for protein digestion and sequence analysis. It is also used in cell culture for cell detachment and passaging. Clinically, trypsin is used for wound debridement and the treatment of various inflammatory conditions. It is also used as a rennet extender in food processing and for dehairing hides. The enzyme is available in various grades and activities (e.g., Trypsin 1:250).
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| Molecular Formula |
C35H47N7O10
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|---|---|
| Molecular Weight |
725.7886
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| CAS # |
9002-07-7
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3778 mL | 6.8890 mL | 13.7781 mL | |
| 5 mM | 0.2756 mL | 1.3778 mL | 2.7556 mL | |
| 10 mM | 0.1378 mL | 0.6889 mL | 1.3778 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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