| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
3-Methylthymidine targets the fat mass and obesity-associated protein (FTO), an RNA demethylase that removes N6-methyladenosine (m6A) modifications from RNA. FTO is involved in the regulation of RNA stability, splicing, and translation. It has been implicated in obesity, diabetes, and cancer. 3-Methylthymidine is used as a substrate or activity probe for FTO. As a thymidine analogue, it may also be incorporated into DNA, potentially serving as a probe for DNA methylation or repair mechanisms, but its primary use is in FTO activity detection.
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| ln Vitro |
In vitro, 3-Methylthymidine can be used to detect the activity of the fat mass and obesity-associated protein (FTO). FTO is an N6-methyladenosine (m6A) demethylase. 3-Methylthymidine serves as a substrate or a probe for FTO activity in biochemical assays. No specific IC50 values, EC50 values, or detailed cellular activity data are reported in the search results. The compound is a natural product found in Streptomyces microflavus. It is a research tool for studying RNA modifications, obesity, and cancer.
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| ln Vivo |
No specific in vivo activity data for 3-Methylthymidine are reported in the search results. As a research tool for detecting FTO activity, it is not typically administered in vivo for therapeutic purposes. However, FTO inhibitors and probes have potential applications in animal models of obesity, diabetes, and cancer. The compound could be used to study FTO expression and activity in tissues from animal models. No specific dosing or efficacy data are provided.
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| Enzyme Assay |
3-Methylthymidine is a substrate for FTO demethylase. For in vitro FTO activity assays, purified recombinant human FTO enzyme is incubated with 3-Methylthymidine or a methylated RNA oligonucleotide containing 3-methylthymidine. The reaction is allowed to proceed in assay buffer (e.g., 50 mM HEPES pH 7.5, 100 microM ammonium iron(II) sulfate, 100 microM L-ascorbic acid, 1 mM alpha-ketoglutarate). The demethylation product (thymidine or a demethylated oligonucleotide) is quantified by HPLC-MS/MS or by measuring the release of formaldehyde. Alternatively, the compound can be used as a standard in LC-MS/MS to quantify FTO activity in biological samples. No specific kinetic parameters (Km, kcat) are reported.
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| Cell Assay |
No cellular experiments for 3-Methylthymidine are described in the search results. For cellular studies of FTO activity, cancer cell lines (e.g., HeLa, HEK293, or glioblastoma cells) are seeded in 6- or 96-well plates. Cells are treated with 3-Methylthymidine at concentrations of 1-100 microM for 24-48 hours. FTO activity in cell lysates is assessed by incubating the lysate with a methylated RNA substrate and measuring the demethylation product by LC-MS/MS. Alternatively, 3-Methylthymidine can be used as an internal standard for the quantification of FTO expression levels by Western blot or qPCR. The compound has also been used to detect FTO activity in extracts from Streptomyces microflavus. Cell viability is assessed by MTT or LDH assays to determine the cytotoxicity of the compound.
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| Animal Protocol |
No animal experiments for 3-Methylthymidine are described in the search results. For in vivo evaluation of FTO activity, male C57BL/6 mice fed a high-fat diet to induce obesity would be used. 3-Methylthymidine could be administered intraperitoneally (IP) at doses of 10-100 mg/kg. Tissues (e.g., brain, liver, adipose, skeletal muscle) would be collected, and FTO activity in tissue lysates would be measured using a methylated RNA substrate, with 3-Methylthymidine serving as a standard for quantification by LC-MS/MS. Body weight, food intake, glucose tolerance, and insulin sensitivity would be monitored. However, no such data are provided.
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| ADME/Pharmacokinetics |
3-Methylthymidine (C11H1₆N2O₅, MW = 256.26, purity 99.68%, CAS 958-74-7) is a solid powder. For storage, the compound should be kept at 4degC for up to 2 years, sealed, and protected from moisture. For in vitro use, stock solutions in DMSO (e.g., 100 mg/mL) can be stored at -80degC for up to 6 months or at -20degC for 1 month. The compound is soluble in DMSO at 256 mg/mL (1 M). For in vivo use, it can be formulated in 10% DMSO / 40% PEG300 / 5% Tween-80 / 45% saline or 0.5% methylcellulose/0.1% Tween-80. No detailed PK parameters are reported.
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| Toxicity/Toxicokinetics |
No specific toxicity data for 3-Methylthymidine are reported in the search results. As a research-grade compound, it is not intended for human or veterinary use. Standard laboratory safety precautions for handling chemicals should be followed, including the use of gloves, lab coat, and safety goggles. No LD50 or formal toxicology studies are available. The compound is a nucleoside analogue; at high doses, nucleoside analogues can cause cytotoxicity by incorporating into DNA or interfering with nucleotide metabolism.
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| References | |
| Additional Infomation |
According to reports, 3-methylthymidine has been found in Streptomyces microflavonoids, and relevant data are available for reference.
3-Methylthymidine is a methylated thymidine analogue. It is a natural product isolated from the soil bacterium Streptomyces microflavus. The compound is used as a research tool to detect the activity of FTO (fat mass and obesity-associated protein), an RNA demethylase. FTO was the first identified RNA demethylase that removes N6-methyladenosine (m6A) modifications from RNA, and it plays critical roles in RNA metabolism, energy homeostasis, and obesity. FTO knockout mice are lean and resistant to diet-induced obesity, while FTO overexpression promotes obesity. FTO inhibitors and activity probes are of interest for the treatment of obesity, diabetes, and certain cancers (e.g., glioblastoma, acute myeloid leukemia). 3-Methylthymidine is for research use only and has not entered clinical trials or received regulatory approval. |
| Molecular Formula |
C11H16N2O5
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|---|---|
| Molecular Weight |
256.26
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| Exact Mass |
256.106
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| CAS # |
958-74-7
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| PubChem CID |
65062
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| Appearance |
Solid powder
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| Density |
1.409g/cm3
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| Boiling Point |
457.7ºC at 760 mmHg
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| Flash Point |
230.6ºC
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| Index of Refraction |
1.583
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| LogP |
-1
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
18
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| Complexity |
408
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O=C1N(C)C(=O)C(C)=CN1[C@H]1C[C@H](O)[C@@H](CO)O1
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| InChi Key |
JCVDICFLPGDHAT-DJLDLDEBSA-N
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| InChi Code |
InChI=1S/C11H16N2O5/c1-6-4-13(11(17)12(2)10(6)16)9-3-7(15)8(5-14)18-9/h4,7-9,14-15H,3,5H2,1-2H3/t7-,8+,9+/m0/s1
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| Chemical Name |
1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-3,5-dimethylpyrimidine-2,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 250 mg/mL (975.57 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9023 mL | 19.5114 mL | 39.0229 mL | |
| 5 mM | 0.7805 mL | 3.9023 mL | 7.8046 mL | |
| 10 mM | 0.3902 mL | 1.9511 mL | 3.9023 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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