| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
PIM1 0.24 μM (IC50) PIM2 10.53 μM (IC50)
|
|---|---|
| ln Vitro |
Pim-1 kinase inhibitor 9 inhibits the growth of breast cancer T47D cells with IC50 values of 9.8 μM (48h) and 2.61 μM (96h) [1]. Pim-1 kinase inhibitor 9 inhibits the activities of Pim-1 kinase and Pim-2 kinase with IC50 values of 0.24 μM (Pim-1 kinase) and 10.53 μM (Pim-2 kinase) [1].
|
| ln Vivo |
Pim-1 kinase inhibitors 9 (1 mg/kg, once a week for three weeks, intraperitoneal injection) inhibited the growth of mouse Ehrlich tumors by promoting the expression of caspase-3 and inhibiting the expression of VEGF [1].
|
| Cell Assay |
Cell Cytotoxicity Assay[1]
Cell Types: T47D Tested Concentrations: 10 μM Incubation Duration: 48 h and 96 h Experimental Results: Exhibited cytotoxicity activity to T47D with IC50 value of 9.8 μM (48 h) and 2.61μM (96h). Cell Cycle Analysis[1] Cell Types: T47D Tested Concentrations: 10 μM Incubation Duration: 48 h Experimental Results: Inhibited cell cycle at S phase. |
| Animal Protocol |
Animal/Disease Models: swiss albino mice/Ehrlich solid carcinoma model[1]
Doses: 1 mg/kg Route of Administration: i.p. injected once a week, for 3 weeks Experimental Results: Inhibited growth of Ehrlich solid tumours in mice. |
| References |
| Molecular Formula |
C36H24F2N4S2
|
|---|---|
| Molecular Weight |
614.73
|
| Appearance |
Typically exists as solid at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6267 mL | 8.1337 mL | 16.2673 mL | |
| 5 mM | 0.3253 mL | 1.6267 mL | 3.2535 mL | |
| 10 mM | 0.1627 mL | 0.8134 mL | 1.6267 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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