| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Cn2 toxin TFA targets the voltage-gated sodium channel (VGSC) subtype Nav1.6. Nav1.6 is a pore-forming subunit of voltage-gated sodium channels that is highly expressed in the central and peripheral nervous systems. It plays a critical role in neuronal excitability and the propagation of action potentials. Cn2 toxin TFA acts as a β-scorpion toxin, which typically binds to the voltage sensor domain of the sodium channel and alters its gating properties. This modulation can lead to hyperexcitability or blockade of the channel, depending on the specific mechanism of the toxin. The compound's specificity for Nav1.6 makes it a valuable tool for studying the function of this channel subtype.
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| ln Vitro |
In vitro, Cn2 toxin TFA is used as a tool to study the function of the Nav1.6 voltage-gated sodium channel. Its activity can be assessed in electrophysiological assays using cells expressing Nav1.6. The toxin modulates the channel's activity by binding to it. As a β-scorpion toxin, it is expected to alter the voltage dependence of channel activation or inactivation. The compound's specificity for Nav1.6 makes it useful for dissecting the role of this channel subtype in neuronal excitability.
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| ln Vivo |
In vivo activity data for Cn2 toxin TFA are not available in the current scientific literature, as it is a toxin used primarily for in vitro research. As a scorpion toxin, it would be expected to have potent neurotoxic effects in vivo, but these are not typically studied in a research context.
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| Enzyme Assay |
Cn2 toxin TFA's activity is typically assessed using electrophysiological techniques. A standard protocol involves expressing Nav1.6 channels in Xenopus oocytes or mammalian cell lines. Whole-cell patch-clamp recordings are performed to measure sodium currents in the presence and absence of the toxin. The toxin is applied to the bath solution at varying concentrations, and its effects on channel gating (e.g., voltage dependence of activation, inactivation) are analyzed.
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| Cell Assay |
Cn2 toxin TFA is not typically used in cell-based assays measuring cell viability or proliferation. Its use is focused on electrophysiological studies of ion channel function.
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| Animal Protocol |
Cn2 toxin TFA is a toxin and is not used in animal models for therapeutic purposes. It is used for in vitro research on ion channels.
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| ADME/Pharmacokinetics |
Cn2 toxin TFA is a peptide toxin with a molecular weight typical for such compounds. It is provided as a TFA salt for solubility. It is not a drug and its pharmacokinetic properties are not relevant.
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| References | |
| Additional Infomation |
Cn2 toxin TFA (β-Mammal toxin Cn2 TFA) is a research-grade neurotoxic peptide used to study the Nav1.6 voltage-gated sodium channel. It is the main toxin in scorpion venom. It has not entered clinical trials and is not approved for any therapeutic indication. Its mechanism of action involves modulation of Nav1.6 channel activity. The compound is available exclusively for research purposes.
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| Molecular Formula |
C336H496N90O96S8.XC2HF3O2
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|---|---|
| Molecular Weight |
7588.60 (free base)
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~50 mg/mL (with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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