| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
PEGs
PROTAC Linkers. |
|---|---|
| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
As a linker molecule, Benzyl-PEG4-MS itself has no intrinsic biological activity; it serves as a structural connector to join a target protein ligand and an E3 ubiquitin ligase ligand in PROTAC synthesis. The mesylate group is an excellent leaving group for nucleophilic substitution (SN2) reactions, allowing the attachment of ligands containing nucleophilic groups such as amines, thiols, or carboxylates. The benzyl group serves as a protecting group for the terminal hydroxyl (which can be deprotected by hydrogenolysis using H2 and Pd/C to reveal a free OH). This free hydroxyl can then be further functionalized (e.g., converted to an amine or tosylate) for additional conjugation. The PEG4 spacer provides hydrophilicity, flexibility, and reduced steric hindrance, which can facilitate the formation of productive ternary complexes. |
| ln Vivo |
No specific in vivo activity has been reported for this linker alone; its activity is derived from the final PROTAC molecule after conjugation with appropriate ligands. The in vivo efficacy of a complete PROTAC is determined in animal models.
|
| Enzyme Assay |
N/A; this compound is not assessed in isolated enzyme/receptor binding assays. As a synthetic intermediate, its quality is typically confirmed by analytical methods such as HPLC and NMR, with a standard purity of ≥95%. The benzyl group can be characterized by its characteristic aromatic proton signals in NMR spectroscopy (multiplet around 7.2-7.4 ppm). The mesylate group can be characterized by its characteristic singlet around 3.0-3.1 ppm for the CH3 group. The IUPAC name is 2-{2-[2-(2-{benzyloxy}ethoxy)ethoxy]ethoxy}ethyl methanesulfonate.
|
| Cell Assay |
N/A; this linker is not tested alone in cell-based assays. It is used as a building block for constructing PROTACs. In a typical synthesis, the mesylate group is displaced by a nucleophile (e.g., a thiol or amine) from a target protein ligand or an E3 ligase ligand in the presence of a base such as K2CO3. The benzyl group can then be removed by hydrogenolysis to reveal a free hydroxyl group, which can be converted to a leaving group (e.g., mesylate or tosylate) and displaced by another nucleophile. The resulting PROTAC is then tested in cells for target degradation activity.
|
| Animal Protocol |
N/A; no animal studies are performed with the linker alone. For a complete PROTAC conjugate, in vivo studies are conducted following institutional guidelines. The compound is typically formulated using a vehicle containing DMSO, PEG300, Tween-80, and saline and administered via intraperitoneal (IP) or intravenous (IV) injection. The PEG4 spacer may improve the pharmacokinetic properties of the conjugate by enhancing water solubility and reducing aggregation.
|
| ADME/Pharmacokinetics |
This compound has a molecular weight of 390.49, a molecular formula of C18H30O7S, and a standard purity of ≥95%. The IUPAC name is 2-{2-[2-(2-{benzyloxy}ethoxy)ethoxy]ethoxy}ethyl methanesulfonate. It appears as a colorless to light yellow liquid. For storage, it should be kept at -20degC for up to 3 years, sealed, away from moisture. It is soluble in DMSO and other organic solvents. Density: approximately 1.2 g/cm3 (predicted). CAS: 477781-69-4.
|
| Toxicity/Toxicokinetics |
This product is for research use only and is not for human or veterinary use. Standard chemical safety precautions should be observed during handling. The mesylate group is reactive and should be handled with care to avoid premature reactions. The product should be stored away from moisture. PROTAC is a registered trademark of Arvinas Operations, Inc., and is used under license. This compound is not an approved drug and has not been cleared for clinical use.
|
| References |
[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562
|
| Additional Infomation |
The combination of a benzyl protecting group and a mesylate leaving group on a PEG4 spacer provides a versatile platform for constructing heterobifunctional PROTACs. The mesylate group is generally more reactive than tosylate toward nucleophiles, enabling faster substitution reactions. The benzyl group can be removed under mild hydrogenolysis conditions without affecting most other functional groups, revealing a free hydroxyl group for further derivatization. This orthogonal protection strategy enables stepwise conjugation to two different ligands. The PEG4 spacer provides optimal length and hydrophilicity for many PROTAC applications. This linker is also widely used in bioconjugation and polymer chemistry.
|
| Molecular Formula |
C16H26O7S
|
|---|---|
| Molecular Weight |
362.44
|
| Exact Mass |
362.139
|
| CAS # |
477781-69-4
|
| PubChem CID |
24180090
|
| Appearance |
Light yellow to yellow viscous liquid
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
497.1±40.0 °C at 760 mmHg
|
| Flash Point |
254.5±27.3 °C
|
| Vapour Pressure |
0.0±1.2 mmHg at 25°C
|
| Index of Refraction |
1.502
|
| LogP |
0.42
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
15
|
| Heavy Atom Count |
24
|
| Complexity |
374
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CS(=O)(=O)OCCOCCOCCOCCOCC1=CC=CC=C1
|
| InChi Key |
DAODDIFNUYIKRO-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C16H26O7S/c1-24(17,18)23-14-13-21-10-9-19-7-8-20-11-12-22-15-16-5-3-2-4-6-16/h2-6H,7-15H2,1H3
|
| Chemical Name |
2-[2-[2-(2-phenylmethoxyethoxy)ethoxy]ethoxy]ethyl methanesulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7591 mL | 13.7954 mL | 27.5908 mL | |
| 5 mM | 0.5518 mL | 2.7591 mL | 5.5182 mL | |
| 10 mM | 0.2759 mL | 1.3795 mL | 2.7591 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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