| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
L-Aspartic acid-15N does not have a specific biological target itself, as it is an isotopically labeled compound. However, its unlabeled counterpart, L-aspartic acid, is a non-essential amino acid that plays a role in the urea cycle, the citric acid cycle, and the synthesis of other amino acids. It is a precursor for the synthesis of asparagine, arginine, lysine, methionine, threonine, and isoleucine. It also acts as a neurotransmitter.
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| ln Vitro |
L-Aspartic acid-15N is not typically used for in vitro activity studies in the same way as pharmacologically active compounds. However, it is used as a tracer in metabolic studies to investigate the metabolism of aspartic acid in cells. It is also used as a precursor in the synthesis of other 15N-labeled compounds. The labeled compound allows for the tracking of the nitrogen atom through metabolic pathways.
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| ln Vivo |
As a labeled compound, L-Aspartic acid-15N is not used for in vivo activity studies as a therapeutic agent. However, it is used as a tracer in metabolic studies in vivo to investigate the metabolism of aspartic acid and the fate of its nitrogen atom. It can be administered to animals to study the incorporation of nitrogen into various metabolites and tissues.
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| Enzyme Assay |
L-Aspartic acid-15N is not typically used in enzyme/receptor binding assays. However, it may be used as a substrate in enzyme assays to study the activity of enzymes that utilize aspartic acid, such as aspartate transaminase or asparagine synthetase. The labeled compound allows for the sensitive detection of the reaction products by mass spectrometry.
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| Cell Assay |
L-Aspartic acid-15N is not used in cell-based assays as a bioactive compound. However, it may be used as a tracer in cell-based metabolic studies. Cells are cultured with the labeled compound, and the incorporation of the 15N label into various metabolites is measured by mass spectrometry. This allows for the study of aspartic acid metabolism and nitrogen flux in cells.
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| Animal Protocol |
In vivo animal studies using L-Aspartic acid-15N are typically conducted as metabolic tracing studies. The labeled compound is administered to animals, and the distribution of the 15N label in various tissues and metabolites is measured. This allows for the study of aspartic acid metabolism and nitrogen balance in vivo.
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| ADME/Pharmacokinetics |
L-Aspartic acid-15N has a molecular formula of C4H7NO4 (with 15N) and a molecular weight of 134.10. The CAS number is 3715-16-0. The compound is typically supplied as a solid and should be stored according to the manufacturer's recommendations. It has an isotopic purity of 98 atom % 15N or higher.
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| Toxicity/Toxicokinetics |
The toxicity profile of L-Aspartic acid-15N is similar to that of unlabeled L-aspartic acid, as it is a stable isotope-labeled amino acid. L-Aspartic acid is a naturally occurring amino acid that is generally recognized as safe. The labeled compound is intended for research use only and is not approved for therapeutic use in humans. Standard laboratory safety practices should be followed.
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| References |
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| Additional Infomation |
L-Aspartic acid-15N (CAS 3715-16-0) is an isotopically labeled form of L-aspartic acid, where the nitrogen atom is enriched with the stable isotope 15N. L-Aspartic acid is an amino acid that has been shown to be a precursor active molecule for colon-specific active molecule delivery. It is used as a tracer in metabolic studies.
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| Molecular Formula |
C4H715NO4
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|---|---|
| Molecular Weight |
134.10
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| Exact Mass |
134.035
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| CAS # |
3715-16-0
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| Related CAS # |
L-Aspartic acid;56-84-8
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| PubChem CID |
16212100
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| Appearance |
White to off-white solid powder
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| Melting Point |
>300ºC (dec.)(lit.)
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| LogP |
-2.8
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
9
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| Complexity |
133
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C([C@@H](C(=O)O)[15NH2])C(=O)O
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| InChi Key |
CKLJMWTZIZZHCS-KPHKZEKTSA-N
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| InChi Code |
InChI=1S/C4H7NO4/c5-2(4(8)9)1-3(6)7/h2H,1,5H2,(H,6,7)(H,8,9)/t2-/m0/s1/i5+1
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| Chemical Name |
(2S)-2-(15N)azanylbutanedioic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.4571 mL | 37.2856 mL | 74.5712 mL | |
| 5 mM | 1.4914 mL | 7.4571 mL | 14.9142 mL | |
| 10 mM | 0.7457 mL | 3.7286 mL | 7.4571 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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