| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Succinic acid-d6 does not have a specific biological target itself, as it is an analytical internal standard. However, its unlabeled counterpart, succinic acid, is a key intermediate in the TCA cycle and plays a crucial role in energy metabolism. It is also a signaling molecule and is involved in various metabolic pathways. The labeled compound is used as an internal standard for the quantification of succinic acid by mass spectrometry.
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| ln Vitro |
Succinic acid-d6 is not typically used for in vitro activity studies in the same way as pharmacologically active compounds. However, it is used as an internal standard in in vitro assays to quantify succinic acid levels in cell culture media, cell lysates, and other biological samples. It is used in studies of energy metabolism, the TCA cycle, and metabolic disorders.
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| ln Vivo |
As an analytical internal standard, succinic acid-d6 is not used for in vivo activity studies. However, it may be used as an internal standard in in vivo studies to quantify succinic acid levels in plasma, urine, and tissues. This is important for studying energy metabolism, the TCA cycle, and related metabolic disorders. The labeled compound allows for accurate quantification of succinic acid in biological samples.
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| Enzyme Assay |
In vitro assays for succinic acid-d6 typically involve its use as an internal standard in analytical methods such as LC-MS/MS or GC-MS. It is added to samples to correct for variability in sample preparation and instrument performance, ensuring accurate quantification of succinic acid. It may also be used in metabolic flux studies to trace the metabolism of succinate in cells.
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| Cell Assay |
Succinic acid-d6 is not used in cell-based assays as a bioactive compound. However, it may be used as an internal standard in cell-based assays that measure the uptake, metabolism, or release of succinic acid from cultured cells. In these applications, the labeled compound is added to cell culture media or cell lysates to enable accurate quantification of succinic acid levels by mass spectrometry.
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| Animal Protocol |
In vivo animal studies using succinic acid-d6 are not typically performed as an active treatment. However, the compound may be administered to animals in metabolic tracing studies to track the fate of succinate in vivo. In such studies, the labeled compound is used to distinguish between endogenous and exogenous succinate and to study its metabolism and distribution in various tissues.
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| ADME/Pharmacokinetics |
Succinic acid-d6 has a molecular formula of C4D6O4 and a molecular weight of 124.13. The CAS number is 21668-90-6. It has an isotopic purity of 98 atom % D. The compound has a purity of ≥98%. It is the deuterated form of succinic acid. The compound is intended for research use only.
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| Toxicity/Toxicokinetics |
The toxicity profile of succinic acid-d6 is similar to that of unlabeled succinic acid, as it is a stable isotope-labeled metabolite. Succinic acid is a naturally occurring metabolite that is generally recognized as safe. The labeled compound is intended for research use only and is not approved for therapeutic use in humans. Standard laboratory safety practices should be followed.
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| References | |
| Additional Infomation |
Succinic acid-d6 (Wormwood acid-d6) (CAS 21668-90-6) is the deuterated form of succinic acid. Succinic acid is an intermediate in the TCA cycle and one of the fermentation products of anaerobic metabolism. It has a molecular formula of C4D6O4 and a molecular weight of 124.13. The compound is intended for research use only.
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| Molecular Formula |
C4D6O4
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|---|---|
| Molecular Weight |
124.13
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| Exact Mass |
124.064
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| CAS # |
21668-90-6
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| Related CAS # |
Succinic acid;110-15-6
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| PubChem CID |
16213392
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
236.1±13.0 °C at 760 mmHg
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| Melting Point |
187-190ºC(lit.)
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| Flash Point |
110.9±16.3 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.478
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| LogP |
-0.59
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
8
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| Complexity |
92.6
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])(C(=O)O[2H])C([2H])([2H])C(=O)O[2H]
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| InChi Key |
KDYFGRWQOYBRFD-DTDGFSOZSA-N
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| InChi Code |
InChI=1S/C4H6O4/c5-3(6)1-2-4(7)8/h1-2H2,(H,5,6)(H,7,8)/i1D2,2D2/hD2
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| Chemical Name |
dideuterio 2,2,3,3-tetradeuteriobutanedioate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~805.61 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.0561 mL | 40.2804 mL | 80.5607 mL | |
| 5 mM | 1.6112 mL | 8.0561 mL | 16.1121 mL | |
| 10 mM | 0.8056 mL | 4.0280 mL | 8.0561 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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