| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 500mg | |||
| Other Sizes |
| Targets |
Sarafloxacin-d8 hydrochloride trihydrate, like its unlabeled counterpart, targets bacterial DNA gyrase and topoisomerase IV, enzymes essential for bacterial DNA replication. As a labeled compound, its primary use is as an analytical standard.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantification throughout the drug development process. Due to its potential to alter the pharmacokinetic and metabolic characteristics of medications, deuteration has drawn attention[1].
In vitro, Sarafloxacin-d8 hydrochloride trihydrate exhibits antibacterial activity identical to unlabeled Sarafloxacin. It is a quinolone antibiotic effective against a range of bacterial pathogens. Its primary in vitro use is as an internal standard for the quantification of Sarafloxacin. |
| ln Vivo |
In vivo, Sarafloxacin-d8 hydrochloride trihydrate behaves identically to unlabeled Sarafloxacin. It is used in veterinary medicine for the treatment of bacterial infections in animals, particularly in poultry and swine. The deuterium label allows for pharmacokinetic studies.
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| Enzyme Assay |
Non-cellular assays for Sarafloxacin-d8 hydrochloride trihydrate are not typically performed, as it is primarily used as an analytical standard. Its use in mass spectrometry involves spiking it into samples to serve as an internal standard for quantifying Sarafloxacin.
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| Cell Assay |
In vitro cellular assays for Sarafloxacin-d8 hydrochloride trihydrate are not typically performed, as its primary application is as an analytical standard. Its antibacterial activity is well-characterized for the unlabeled compound.
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| Animal Protocol |
In vivo animal experiments for Sarafloxacin-d8 hydrochloride trihydrate would involve administering the compound to animals to study the pharmacokinetics of Sarafloxacin. The deuterium label allows for precise tracking of the drug and its metabolites in biological samples.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) properties: Sarafloxacin-d8 hydrochloride trihydrate behaves identically to unlabeled Sarafloxacin. Its PK profile in target animal species (e.g., poultry, swine) would be similar, allowing for accurate pharmacokinetic studies using the labeled compound as a tracer.
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| Toxicity/Toxicokinetics |
Toxicological profile: Sarafloxacin-d8 hydrochloride trihydrate is a labeled form of a quinolone antibiotic. Its safety profile is expected to be similar to that of Sarafloxacin, which is used in veterinary medicine. It is intended for research use only and not for human consumption.
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| References | |
| Additional Infomation |
Sarafloxacin-d8 hydrochloride trihydrate has a molecular weight of 483.92. Its CAS number is not provided in the available sources, but the unlabeled Sarafloxacin hydrochloride trihydrate has a CAS number of 91296-87-6. It is supplied for research purposes as an analytical standard.
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| Molecular Formula |
C20H16D8CLF2N3O6
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| Molecular Weight |
483.92
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| Related CAS # |
Sarafloxacin hydrochloride;91296-87-6;Sarafloxacin;98105-99-8
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0665 mL | 10.3323 mL | 20.6646 mL | |
| 5 mM | 0.4133 mL | 2.0665 mL | 4.1329 mL | |
| 10 mM | 0.2066 mL | 1.0332 mL | 2.0665 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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