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250mg | ||
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ln Vitro |
Compound 6c, also known as HCVcc-IN-2, exhibits antiviral activities against a range of viruses, including coxsackievirus B4 (CBV4), hepatitis A virus HM 175 (HAV), hepatitis C genotype 4 (HCVcc), adenovirus type 7 (HAdV7), and herpes simplex virus 1 (HSV-1) with respective viral reduction rates of 83.3%, 63.3%, 40%, 63.3%, and 30%[1]. HCVcc-IN-2 (0.55-1.9 μg/mL; 1 h) inhibits a range of viruses with IC50 values of 0.55 μg/mL for herpes simplex virus, 0.76 μg/mL for HCVcc genotype virus, and 0.76 μg/mL for coxsackievirus B4), respectively; or CC50 values of 1.8 μg/mL for herpes simplex virus, 1.9 μg/mL for HCVcc genotype virus, and 1.9 μg/mL for coxsackievirus B4), respectively[1]. With IC50s of 16.01 μg/mL and 7.68 μg/mL, respectively, HCVcc-IN-2 (7.68-16.01 μg/mL) inhibits the hepatitis C virus NS3/4A and HSV-USP7 protease enzyme[1]. HCVcc-IN-2(0.01 mM; 24 h) exhibits strong inhibition against two cell lines, SK-MEL-5 and OVCAR-4, while having minimal cytotoxicity[1].
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Cell Assay |
Cell Cytotoxicity Assay[1]
Cell Types: FRHK-4, Hep2, BGM, Vero, and Huh 7.5 cell lines Tested Concentrations: 100 μg/mL Incubation Duration: 24 hrs (hours) Experimental Results: demonstrated nontoxic under the doses of 100 μg/mL against FRHK-4, Hep2, BGM, Vero, and Huh 7.5 cells, respectively. Cell Viability Assay[1] Cell Types: SF- 539, SNB-75, HCT-116, and A498 Tested Concentrations: 0.01 mM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibited SF-539, SNB-75, HCT-116, and A498 cell viability by 15.70%, 16.66%, 75.89%, and 58.5%, respectively. |
References |
[1]. Azzam et al. Novel Thiophene Thioglycosides Substituted with the Benzothiazole Moiety: Synthesis, Characterization, Antiviral and Anticancer Evaluations, and NS3/4A and USP7 Enzyme Inhibitions. ACS Omega. 2022 Sep 29;7(40):35656-35667.
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Molecular Formula |
C32H29BRN2O10S3
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Molecular Weight |
777.68
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.2859 mL | 6.4294 mL | 12.8588 mL | |
5 mM | 0.2572 mL | 1.2859 mL | 2.5718 mL | |
10 mM | 0.1286 mL | 0.6429 mL | 1.2859 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.