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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
The antifungal agent 41, which has a MIC value of 2, 1, 8, 2, 8, 0.25, 4, and 1 μg/mL, exhibits antifungal activity against C. alb, C. alb(sc5314), C.gla, C.par, C.kru, C.zey, C.neo, and Af[1]. With MIC values of 2, 8, 4, 2, and 4 μg/mL for strains CaR, 17#, 632, 901, and 904, respectively, antifungal agent 41 (0-64 μg/mL; 24 h) has antifungal activity against pathogenic fluconazole-resistant Candida albicans[1]. Against Candida albicans, antifungal agent 41 (0-64 μg/mL; 24 h) exhibits antifungal activity with MFC values of 16, 8, 16, >64, 16, 2, and 4 μg/mL for C. albicans, C. albicans(sc5314), strain CaR, 17#, 632, 901, and 904, respectively[1]. With SMIC50s of 0.5 μg/mL and 0.5-4 μg/mL, as well as SMIC80s of 4-32 μg/mL and 2-4 μg/mL, respectively, antifungal agent 41 (0-1024 μg/mL; 1.5-24 h) suppresses the biofilms of C.alb ATCC SC5314 and CPCC400616[1].
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ln Vivo |
In vivo antifungal activities are demonstrated using antifungal agent 4 (6–12 mg/kg; intraperitoneally once daily for five days)[1].
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Cell Assay |
Cell Cytotoxicity Assay[1]
Cell Types: HL-60, MDA-MB-231 and PC-3 cell lines Tested Concentrations: 0-100 μM Incubation Duration: 24 h Experimental Results: Inhibited growth of HL-60, MDA-MB-231 and PC-3 cells with IC50s of 32.53, 6.25 and 1.43 μM, respectively. |
Animal Protocol |
Animal/Disease Models: Mice with C.alb ATCC SC5314 infection[1]
Doses: 6 mg/kg and 12 mg /kg Route of Administration: intraperitoneal (ip)injection; 6 mg/kg and 12mg/kg one time/day; for 5 days Experimental Results: Dramatically decreased the kidney fungal burden of C.alb. |
References |
[1]. Xu H, et al. Discovery of novel selenium-containing azole derivatives as antifungal agents by exploiting the hydrophobic cleft of CYP51. Eur J Med Chem. 2022 Aug 28;243:114707.
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Molecular Formula |
C22H18CL4N4SE2
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Molecular Weight |
638.14
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.5671 mL | 7.8353 mL | 15.6705 mL | |
5 mM | 0.3134 mL | 1.5671 mL | 3.1341 mL | |
10 mM | 0.1567 mL | 0.7835 mL | 1.5671 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.