| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
ACTH receptor (Melanocortin 2 receptor, MC2R)
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| ln Vitro |
In freshly dispersed or cultivated beef adrenal cortical cells, ACTH(11–24) (acetate) stimulates submaximally cortisol secretion[1].
In freshly dispersed or cultured beef adrenal cortical cells, ACTH (11-24) (acetate) elicits cortisol secretion, but only submaximally. It is utilized in research to study the central nervous system and the structure-function relationships of ACTH fragments. |
| ln Vivo |
Fighting behavior and the levels of circulating plasma corticosterone are slightly influenced by ACTH (11-24) (acetate)[2].
ACTH (11-24) (acetate) has been shown to have slight influences on circulating plasma corticosterone levels and on fighting behavior in animal models. Its in vivo effects are consistent with its partial antagonism of the native ACTH hormone, modulating the physiological stress response. |
| Enzyme Assay |
In a typical in vitro receptor binding assay, the compound is first dissolved in a suitable buffer. Membranes prepared from cells expressing the ACTH receptor are incubated with a radiolabeled ACTH ligand (e.g., [125I]-ACTH) and increasing concentrations of the unlabeled ACTH (11-24) peptide. After a defined incubation period, the reaction mixture is filtered, and the radioactivity retained on the filters is measured. The peptide's binding affinity (Ki) is then determined from the competition curves.
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| Cell Assay |
For in vitro cell-based assays, primary adrenal cortical cells are isolated from beef adrenal glands. The cells are seeded into culture plates and allowed to attach. The cells are then treated with various concentrations of ACTH (11-24) (acetate) for a specified time (e.g., 2 hours). After treatment, the culture supernatant is collected, and the concentration of cortisol is measured using an ELISA or radioimmunoassay to assess the peptide's partial agonistic/antagonistic activity.
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| Animal Protocol |
A typical in vivo protocol involves male laboratory mice. ACTH (11-24) (acetate) is dissolved in a suitable vehicle (e.g., saline) and administered intraperitoneally at a defined dose. After a set time (e.g., 30-60 minutes), blood samples are collected via the tail vein or cardiac puncture. Plasma is separated, and corticosterone levels are measured by ELISA. Simultaneously, the fighting behavior of the mice is scored over a defined observation period.
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| ADME/Pharmacokinetics |
Specific pharmacokinetic data for ACTH (11-24) (acetate) have not been published. As a peptide fragment, it is expected to be rapidly cleared from systemic circulation. In research applications, it is typically administered via intraperitoneal or intravenous injection to ensure bioavailability. Detailed ADME studies are not standard for a research-grade peptide.
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| Toxicity/Toxicokinetics |
No formal toxicological studies have been reported for this research product. At high doses or with prolonged use, potential toxicity may arise from sustained antagonism of the ACTH receptor, which could suppress cortisol production and lead to adrenal insufficiency. Handling should be performed with gloves and appropriate safety measures.
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| References | |
| Additional Infomation |
ACTH (11-24) (acetate) is a research tool for studying the central nervous system and ACTH receptor function. The full-length ACTH is used clinically to treat infantile spasms and to diagnose adrenal insufficiency. This fragment is not approved for any clinical use. It is provided for research purposes only.
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| Molecular Formula |
C79H138N24O18
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|---|---|
| Molecular Weight |
1712.09
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| Related CAS # |
ACTH (11-24);4237-93-8
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| Appearance |
Solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~50 mg/mL (~29.20 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.5841 mL | 2.9204 mL | 5.8408 mL | |
| 5 mM | 0.1168 mL | 0.5841 mL | 1.1682 mL | |
| 10 mM | 0.0584 mL | 0.2920 mL | 0.5841 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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