| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
CD117 (c-Kit), the receptor for stem cell factor (SCF/steel factor). Binding of SCF to CD117 induces receptor dimerization and autophosphorylation, activating downstream signaling pathways (PI3K/Akt, MAPK, JAK/STAT) critical for hematopoiesis, proliferation, and differentiation of stem cells. The antibody can block SCF binding and deplete c-Kit+ cells.
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| ln Vitro |
The antibody binds specifically to CD117-expressing cells (e.g., mouse bone marrow hematopoietic stem cells). Some clones (e.g., ACK2) have been reported to block c-Kit function and are used for in vivo c-Kit+ cell depletion and in vitro c-Kit neutralization. Binding is typically assessed by flow cytometry, immunoprecipitation, or immunofluorescence.
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| ln Vivo |
In vivo administration of anti-mouse CD117 antibodies (e.g., ACK2) results in depletion of c-Kit+ cells, including hematopoietic stem cells and mast cells. This depletion has been used to block hematopoiesis and disrupt melanocyte development. The antibodies have been used to study the role of c-Kit signaling in stem cell biology and mast cell-mediated allergic responses.
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| Enzyme Assay |
Not applicable. Binding affinity can be measured using recombinant mouse CD117 extracellular domain in ELISA or by surface plasmon resonance (SPR). For blocking assays, SCF-induced CD117 autophosphorylation in purified c-Kit protein or membrane preparations can be measured by Western blot using anti-phospho-c-Kit antibodies.
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| Cell Assay |
Flow cytometry: Mouse bone marrow or spleen cells are stained with the anti-mouse CD117 antibody (directly conjugated to a fluorophore). CD117+ hematopoietic stem and progenitor cells are identified and quantified. For functional assays, cells are treated with the antibody in vitro to block SCF-induced signaling, and downstream markers (e.g., p-Akt, p-ERK) are analyzed by Western blot.
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| Animal Protocol |
In vivo depletion: Mice are injected intraperitoneally with anti-mouse CD117 antibody (clone ACK2 or 3C1). Depletion efficiency is verified by flow cytometry of bone marrow for CD117+ cells. Depleted mice are used to study the role of c-Kit+ stem cells in hematopoiesis, or to create niches for transplanted stem cells. Mast cell depletion models are also used.
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| ADME/Pharmacokinetics |
As a monoclonal antibody, anti-mouse CD117 antibodies have a half-life of approximately 5-10 days in mice. Depletion of CD117+ cells occurs within 24-72 hours and can be sustained for several days to a week, depending on the dose and the rate of stem cell regeneration.
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| Toxicity/Toxicokinetics |
No detailed toxicity data. At standard depletion doses, the antibody is well-tolerated. On-target effects include ablation of hematopoietic stem cells, leading to transient or prolonged cytopenias (anemia, neutropenia, thrombocytopenia), requiring supportive care (e.g., in germ-free or antibiotic-treated environments). Toxicity may be clone-dependent.
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| Additional Infomation |
Anti-mouse CD117 antibodies are research-grade tools, not approved for clinical use. The ACK2 clone blocks c-Kit function and is widely used for depletion. The 2B8 clone does not block SCF binding. CD117 is a therapeutic target in cancer (e.g., gastrointestinal stromal tumors) where imatinib is used. Strictly for research.
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| Appearance |
Colorless to light yellow liquid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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