| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
This compound is a labeling analog; unlabeled Demethoxycurcumin is a natural curcuminoid with effects on multiple cellular targets including NF-kappaB, COX-2, and various kinases.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantification throughout the drug development process. Due to its potential to alter the pharmacokinetic and metabolic characteristics of medications, deuteration has drawn attention[2].
Unlabeled Demethoxycurcumin possesses anti-inflammatory, antioxidant, and anticancer properties; as a stable isotope tracer, the labeled analog has identical biochemical activity but is used primarily for analytical quantification. |
| ln Vivo |
Demethoxycurcumin has anti-inflammatory and toxic effects on cancer cells in animal models; the d4-labeled compound is used for metabolic tracing and PK studies via LC-MS/MS rather than efficacy studies.
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| Enzyme Assay |
Receptor binding is not applicable; as an internal standard, Demethoxycurcumin-d4 is used in analytical method validation for metabolite quantification in biological matrices.
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| Cell Assay |
Cancer cells or primary cells are treated with unlabeled Demethoxycurcumin (1-50 microM) to assess cytotoxicity, apoptosis, or NF-kappaB inhibition; the labeled analog is not used for activity assessment.
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| Animal Protocol |
Animals are administered Demethoxycurcumin orally or intraperitoneally; plasma and tissue samples are collected, extracted, and analyzed by LC-MS/MS using Demethoxycurcumin-d4 as internal standard to correct for matrix effects.
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| ADME/Pharmacokinetics |
PK properties of unlabeled Demethoxycurcumin: poor oral bioavailability due to rapid metabolism and low aqueous solubility; the d4-labeled analog is used to enable accurate quantification in PK studies.
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| Toxicity/Toxicokinetics |
Unlabeled Demethoxycurcumin is considered safe at dietary levels; high-dose toxicity studies are limited; the labeled analog is for research use only and is safe as an analytical standard.
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| References | |
| Additional Infomation |
Demethoxycurcumin is a dietary curcuminoid under preclinical investigation; the d4-labeled version is an analytical standard and not a marketed drug.
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| Molecular Formula |
C20H14D4O5
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|---|---|
| Molecular Weight |
342.38
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| Related CAS # |
Demethoxycurcumin;22608-11-3
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9207 mL | 14.6037 mL | 29.2073 mL | |
| 5 mM | 0.5841 mL | 2.9207 mL | 5.8415 mL | |
| 10 mM | 0.2921 mL | 1.4604 mL | 2.9207 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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