| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Dinotefuran acts on nicotinic acetylcholine receptors (nAChRs) in the insect central nervous system; it binds to these receptors, leading to overstimulation, paralysis, and death in target pests.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantification throughout the drug development process. Due to its potential to alter the pharmacokinetic and metabolic characteristics of medications, deuteration has drawn attention[1].
The labeled compound is not evaluated for insecticidal activity; the unlabeled Dinotefuran is a potent nAChR agonist with activity against aphids, whiteflies, thrips, leafhoppers, and leafminers at low ppm concentrations. |
| ln Vivo |
In vivo activity matches the unlabeled insecticide; Dinotefuran is effective in crop protection against a wide range of sucking and chewing insect pests via foliar, soil, and seed treatment applications.
|
| Enzyme Assay |
Receptor binding is typically assessed by radioligand binding assays using insect nAChR membrane preparations; the labeled version is not used in such assays but serves as an LC-MS internal standard.
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| Cell Assay |
Insect cell lines (e.g., Sf9 expressing nAChR subunits) or neuronal cultures are exposed to Dinotefuran to measure intracellular calcium influx or membrane depolarization; the labeled analog is not used for activity assessment.
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| Animal Protocol |
Dinotefuran is applied to plants or insects; tissues are homogenized, extracted with organic solvents, and analyzed by LC-MS/MS using Dinotefuran-d3 as an internal standard to compensate for extraction efficiency and matrix effects.
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| ADME/Pharmacokinetics |
PK studies use the deuterated analog as an internal standard to measure Dinotefuran concentrations in plasma, urine, or plant tissues; the unlabeled compound is rapidly absorbed and eliminated with a short half-life in mammals.
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| Toxicity/Toxicokinetics |
Dinotefuran is considered low in toxicity to mammals (acute oral LD50 >5,000 mg/kg in rats) but highly toxic to honeybees and other beneficial insects; the labeled compound has a similar safety profile.
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| References | |
| Additional Infomation |
Dinotefuran is a registered insecticide for agricultural use; the d3-labeled version is a research-grade analytical standard and not for human or veterinary therapeutic use.
|
| Molecular Formula |
C7H11D3N4O3
|
|---|---|
| Molecular Weight |
205.23
|
| CAS # |
2934813-27-9
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| Related CAS # |
Dinotefuran;165252-70-0
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8726 mL | 24.3629 mL | 48.7258 mL | |
| 5 mM | 0.9745 mL | 4.8726 mL | 9.7452 mL | |
| 10 mM | 0.4873 mL | 2.4363 mL | 4.8726 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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