| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Endo-1,4-β-xylanase targets (1→4)-β-D-xylosidic linkages in xylans, which are complex polysaccharides found in plant cell walls. The enzyme catalyzes the endohydrolysis of these linkages, breaking down xylan polymers into smaller xylooligosaccharides and xylose. As a glycoside hydrolase, it belongs to a large family of enzymes that hydrolyze glycosidic bonds in carbohydrates. The enzyme exhibits specificity for β-D-xylan substrates.
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|---|---|
| ln Vitro |
In vitro, Endo-1,4-β-xylanase exhibits robust enzymatic activity in hydrolyzing (1→4)-β-D-xylosidic linkages in xylans. The enzyme's activity can be measured by the release of reducing sugars from xylan substrates using colorimetric assays such as the dinitrosalicylic acid (DNS) method. It is widely used in biochemical research to study carbohydrate metabolism, plant cell wall degradation, and for the production of xylooligosaccharides.
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| ln Vivo |
In vivo activity data for Endo-1,4-β-xylanase are not applicable in the context of a therapeutic agent, as this enzyme is a research tool and industrial enzyme rather than a drug. In its native context, the enzyme is produced by microorganisms to degrade plant cell wall polysaccharides for nutritional purposes. The enzyme may be used in animal feed additives to improve nutrient digestibility.
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| Enzyme Assay |
For non-cellular in vitro enzyme assays, Endo-1,4-β-xylanase is evaluated using purified enzyme and a xylan substrate. The reaction is typically carried out in a suitable buffer at optimal pH and temperature. Enzyme activity is measured by the release of reducing sugars from the xylan substrate using colorimetric methods such as the DNS assay. The specific activity and kinetic parameters (Km, Vmax) are determined.
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| Cell Assay |
For in vitro cellular assays, Endo-1,4-β-xylanase is not typically used in mammalian cell culture as a therapeutic agent. However, its activity can be studied in microbial cultures or in plant cell wall degradation models. The enzyme's function in cells or complex substrates is assessed by analyzing the degradation products released from xylan.
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| Animal Protocol |
In vivo animal studies are not applicable for Endo-1,4-β-xylanase as a therapeutic drug. However, the enzyme may be studied in animal models for its effects on nutrient digestibility when used as a feed additive. In such studies, animals are fed diets containing the enzyme, and digestibility parameters, growth performance, and gut health are assessed.
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| ADME/Pharmacokinetics |
Pharmacokinetic data are not applicable for Endo-1,4-β-xylanase, as it is not a therapeutic drug but a research and industrial enzyme. The enzyme is typically used in controlled in vitro conditions or as a feed additive and is not administered systemically for therapeutic purposes. Its stability and activity are assessed under various pH and temperature conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for Endo-1,4-β-xylanase are primarily related to its use as a food enzyme and feed additive. The enzyme is generally recognized as safe for its intended uses. Standard safety assessments for enzyme preparations include studies on allergenicity, toxicity, and potential for causing adverse effects. The enzyme is for research use only and not for human therapeutic use.
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| References | |
| Additional Infomation |
Endo-1,4-β-xylanase is an aromatic amine.
Endo-1,4-β-xylanase (Xylanase; CtXyn11A; EC 3.2.1.8) is an arabinoxylan-degrading enzyme and glycoside hydrolase. It catalyzes the endohydrolysis of (1→4)-β-D-xylosidic linkages in xylans. The enzyme has a CAS number of 9025-57-4 and is widely used in biochemical research and industrial applications. Its systematic name is 4-β-D-xylan xylanohydrolase. |
| Molecular Formula |
C34H50N4O2+2
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|---|---|
| Molecular Weight |
546.8
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| Exact Mass |
546.393
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| CAS # |
9025-57-4
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| Related CAS # |
311-09-1 (di-Chloride)
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| PubChem CID |
9394
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| Appearance |
Yellow to brown solid powder
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| LogP |
5.7
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
18
|
| Heavy Atom Count |
40
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| Complexity |
774
|
| Defined Atom Stereocenter Count |
0
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| SMILES |
CC[N+](CC1=CC=CC=C1)(CC)CCCNC1=CC(=O)C(NCCC[N+](CC2=CC=CC=C2)(CC)CC)=CC1=O
|
| InChi Key |
YERABYSOHUZTPQ-UHFFFAOYSA-P
|
| InChi Code |
InChI=1S/C34H48N4O2/c1-5-37(6-2,27-29-17-11-9-12-18-29)23-15-21-35-31-25-34(40)32(26-33(31)39)36-22-16-24-38(7-3,8-4)28-30-19-13-10-14-20-30/h9-14,17-20,25-26H,5-8,15-16,21-24,27-28H2,1-4H3/p+2
|
| Chemical Name |
benzyl-[3-[[4-[3-[benzyl(diethyl)azaniumyl]propylamino]-3,6-dioxocyclohexa-1,4-dien-1-yl]amino]propyl]-diethylazanium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8288 mL | 9.1441 mL | 18.2882 mL | |
| 5 mM | 0.3658 mL | 1.8288 mL | 3.6576 mL | |
| 10 mM | 0.1829 mL | 0.9144 mL | 1.8288 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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