Size | Price | Stock | Qty |
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1mg |
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Other Sizes |
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Targets |
P2Y2 Receptor
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ln Vitro |
MRS2768 (0.01-10000 μM; 24 hours) greatly boosts the proliferation of PANC-1 cells[1].
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ln Vivo |
In mice, myocardial injury is reduced by pretreatment with MRS2768 (4.44 μg/kg iv). Heart muscle cells are shielded from ischemia injury in vivo by MRS2768[2].
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Cell Assay |
Cell Proliferation Assay[1]
Cell Types: Human pancreatic duct epithelial cells PANC-1 Tested Concentrations: 0.01, 0.1, 1, 10, 100, 1000, 10000 μM Incubation Duration: 24 hrs (hours) Experimental Results: The effect on proliferation of PANC-1 cells was dependent on concentration (0.1 μM to 1 mM). The concentration that elicited a half-maximal response (EC50) in the stimulation of proliferation was 0.8±1.7 μM. Resulted in a dose- and time-dependent increase of proliferation in PANC-1 cells |
Animal Protocol |
Animal/Disease Models: Male wild-type mice (C57BL)[2]
Doses: 4.44 μg/ kg Route of Administration: Injected iv; 1 h before myocardial infarct (MI) Experimental Results: Pretreatment decreased myocardial damage. The damage was Dramatically smaller in mice compared to the untreated mice (25.6±4.5% vs. 39.2±6.3%). |
References |
[1]. Ji Hun Choi, et al. Uridine Triphosphate Increases Proliferation of Human Cancerous Pancreatic Duct Epithelial Cells by Activating P2Y2 Receptor. Pancreas. 2013 May;42(4):680-6.
[2]. Edith Hochhauser, et al. P2Y2 Receptor Agonist With Enhanced Stability Protects the Heart From Ischemic Damage in Vitro and in Vivo. Purinergic Signal. 2013 Dec;9(4):633-42. |
Molecular Formula |
C15H16N2NA4O18P4
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Molecular Weight |
728.14
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CAS # |
2567869-47-8
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.3734 mL | 6.8668 mL | 13.7336 mL | |
5 mM | 0.2747 mL | 1.3734 mL | 2.7467 mL | |
10 mM | 0.1373 mL | 0.6867 mL | 1.3734 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.