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| 5mg |
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| Targets |
CYP1
The primary targets of Resorufin methyl ether are cytochrome P450 enzymes, particularly CYP1A2. The compound is a substrate for these enzymes, and its metabolism results in the production of the fluorescent product resorufin. By measuring the fluorescence of resorufin, researchers can quantify CYP450 enzyme activity in vitro. The compound is a relatively specific substrate for CYP1A2 activity in rodents. |
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| ln Vitro |
In vitro, Resorufin methyl ether is used as a fluorogenic substrate to measure CYP450 enzyme activity. The compound is metabolized by CYP1A2 and other CYP450 enzymes to produce the fluorescent product resorufin. The fluorescence can be measured using a fluorometer or microplate reader, allowing for the quantification of enzyme activity. The compound is a relatively specific substrate for CYP1A2 activity in rodents.
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| ln Vivo |
Resorufin methyl ether does not have specific in vivo biological activity as it is used as a substrate for in vitro enzyme assays. However, the compound may be used in ex vivo studies to measure CYP450 activity in tissue samples or microsomal preparations. The compound's utility lies in its ability to serve as a probe for CYP450 enzyme activity.
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| Enzyme Assay |
The in vitro enzyme assay for Resorufin methyl ether involves incubating the compound with CYP450 enzyme preparations (e.g., liver microsomes, recombinant CYP450 isoforms) in the presence of NADPH. The enzyme catalyzes the O-dealkylation of Resorufin methyl ether to produce the fluorescent product resorufin. The fluorescence is measured at excitation and emission wavelengths appropriate for resorufin (typically around 530 nm excitation and 585 nm emission). Enzyme activity is calculated based on the rate of resorufin formation, and inhibition or induction of enzyme activity can be assessed by comparing treated and control samples.
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| Cell Assay |
Cellular assays for Resorufin methyl ether typically use hepatocyte cell lines (e.g., HepG2) or primary hepatocytes that express CYP450 enzymes. Cells are treated with the compound, and the production of fluorescent resorufin is measured to assess CYP450 enzyme activity. The compound can be used to study enzyme induction or inhibition in a cellular context.
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| Animal Protocol |
Resorufin methyl ether does not have specific in vivo animal study protocols as it is used as a substrate for in vitro enzyme assays. The compound may be used in ex vivo studies to measure CYP450 activity in tissue samples or microsomal preparations from animals treated with enzyme inducers or inhibitors.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
7-Methoxysilane's known metabolites include silane. As a fluorogenic substrate, Resorufin methyl ether does not have conventional pharmacokinetic properties. The compound has a molecular weight of 227.22, a melting point of ≥220°C, and is soluble in DMF. It is typically stored at 2-8°C. For research purposes, the compound is handled as a solid with standard safety precautions. |
| Toxicity/Toxicokinetics |
There is no specific toxicity data reported for Resorufin methyl ether in the available literature. As a research chemical, the compound is intended for laboratory use only and should be handled with standard safety precautions. The compound is typically stored at 2-8°C.
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| References |
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| Additional Infomation |
Resorufin methyl ether (Methoxyresorufin) is a fluorogenic substrate for CYP1A2 and other cytochrome P450 enzymes. The compound has a molecular formula of C13H9NO3 and a molecular weight of 227.22. It is metabolized by CYP1A2 to produce the fluorescent product resorufin, allowing for the quantification of enzyme activity. Resorufin methyl ether is a relatively specific substrate for CYP1A2 activity in rodents.
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| Molecular Formula |
C13H9NO3
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|---|---|
| Molecular Weight |
227.22
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| Exact Mass |
227.058
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| CAS # |
5725-89-3
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| PubChem CID |
119220
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| Appearance |
Brown to reddish brown solid powder
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| Density |
1.33 g/cm3
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| Boiling Point |
378.8ºC at 760 mmHg
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| Melting Point |
≥220ºC(lit.)
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| Flash Point |
187.3ºC
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| Index of Refraction |
1.638
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| LogP |
2.301
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
17
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| Complexity |
436
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=CC2=C(C=C1)N=C3C=CC(=O)C=C3O2
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| InChi Key |
KNYYMGDYROYBRE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3
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| Chemical Name |
7-methoxyphenoxazin-3-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMF: 3.33 mg/mL (14.66 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4010 mL | 22.0051 mL | 44.0102 mL | |
| 5 mM | 0.8802 mL | 4.4010 mL | 8.8020 mL | |
| 10 mM | 0.4401 mL | 2.2005 mL | 4.4010 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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