| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
The primary target of 4-Methylumbelliferyl α-L-arabinofuranoside is α-L-arabinofuranosidase, an enzyme that catalyzes the hydrolysis of terminal non-reducing α-L-arabinofuranoside residues from various substrates. The compound serves as a substrate for this enzyme, and its hydrolysis results in the release of the fluorescent product 4-methylumbelliferone. By measuring the fluorescence of 4-methylumbelliferone, researchers can quantify α-L-arabinofuranosidase activity.
|
|---|---|
| ln Vitro |
In vitro, 4-Methylumbelliferyl α-L-arabinofuranoside is used as a fluorogenic substrate to measure α-L-arabinofuranosidase activity. The enzyme catalyzes the hydrolysis of the glycosidic bond, releasing 4-methylumbelliferone, which produces a blue fluorescence. The fluorescence is measured at excitation and emission wavelengths appropriate for 4-methylumbelliferone (typically around 360 nm excitation and 450 nm emission). Enzyme activity is calculated based on the rate of 4-methylumbelliferone production.
|
| ln Vivo |
4-Methylumbelliferyl α-L-arabinofuranoside does not have specific in vivo biological activity as it is used as a substrate for in vitro enzyme assays. However, the compound may be used in ex vivo studies to measure α-L-arabinofuranosidase activity in tissue samples or biological fluids. The compound's utility lies in its ability to serve as a probe for α-L-arabinofuranosidase enzyme activity.
|
| Enzyme Assay |
The in vitro enzyme assay for 4-Methylumbelliferyl α-L-arabinofuranoside involves incubating the compound with α-L-arabinofuranosidase enzyme preparations (e.g., tissue homogenates, cell lysates, or recombinant enzyme) in a suitable buffer system. The enzyme catalyzes the hydrolysis of the substrate to release the fluorescent product 4-methylumbelliferone. The fluorescence is measured at excitation and emission wavelengths appropriate for 4-methylumbelliferone. Enzyme activity is calculated based on the rate of 4-methylumbelliferone formation.
|
| Cell Assay |
Cellular assays for 4-Methylumbelliferyl α-L-arabinofuranoside typically use cell lines that express α-L-arabinofuranosidase. Cells are lysed, and the lysate is incubated with the substrate. The production of 4-methylumbelliferone is measured to quantify enzyme activity. Alternatively, live cells can be used to measure the hydrolysis of the substrate, which may be taken up by the cells. The compound's utility lies in its role as a substrate for α-L-arabinofuranosidase activity measurements.
|
| Animal Protocol |
4-Methylumbelliferyl α-L-arabinofuranoside does not have specific in vivo animal study protocols as it is used as a substrate for in vitro enzyme assays. The compound may be used in ex vivo studies to measure α-L-arabinofuranosidase activity in tissue samples from animals.
|
| ADME/Pharmacokinetics |
As a fluorogenic substrate, 4-Methylumbelliferyl α-L-arabinofuranoside does not have conventional pharmacokinetic properties. The compound is typically stored under standard laboratory conditions. For research purposes, the compound is handled as a solid with standard safety precautions.
|
| Toxicity/Toxicokinetics |
There is no specific toxicity data reported for 4-Methylumbelliferyl α-L-arabinofuranoside in the available literature. As a research chemical, the compound is intended for laboratory use only and should be handled with standard safety precautions. The compound is typically stored under standard laboratory conditions.
|
| References | |
| Additional Infomation |
4-Methylumbelliferyl α-L-arabinofuranoside (CAS#: 77471-44-4) is a synthetic glycoside commonly used as a substrate in biochemical assays, particularly for the detection of α-L-arabinofuranosidase activity. It is a fluorogenic substrate for alpha-L-arabinofuranosidase, producing a blue fluorescent solution upon cleavage. The compound is ideal for biochemical and glycosidase research applications. It is available with a purity of 95%.
|
| Molecular Formula |
C15H16O7
|
|---|---|
| Molecular Weight |
308.28
|
| Exact Mass |
308.09
|
| CAS # |
77471-44-4
|
| PubChem CID |
14161014
|
| Appearance |
White to off-white solid powder
|
| Density |
1.486g/cm3
|
| Boiling Point |
599.4ºC at 760 mmHg
|
| Flash Point |
228.1ºC
|
| Index of Refraction |
1.631
|
| LogP |
-0.2
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
22
|
| Complexity |
463
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
CC1=CC(=O)OC2=C1C=CC(=C2)O[C@H]3[C@@H]([C@H]([C@@H](O3)CO)O)O
|
| InChi Key |
FAGLTVBWEMHJRP-SPWCGHHHSA-N
|
| InChi Code |
InChI=1S/C15H16O7/c1-7-4-12(17)21-10-5-8(2-3-9(7)10)20-15-14(19)13(18)11(6-16)22-15/h2-5,11,13-16,18-19H,6H2,1H3/t11-,13-,14+,15+/m0/s1
|
| Chemical Name |
7-[(2S,3R,4R,5S)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-4-methylchromen-2-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2438 mL | 16.2190 mL | 32.4380 mL | |
| 5 mM | 0.6488 mL | 3.2438 mL | 6.4876 mL | |
| 10 mM | 0.3244 mL | 1.6219 mL | 3.2438 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
