| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
The primary targets of β-Glucosidase are its substrates, which include beta-D-glucosides and other glycosides. The enzyme catalyzes the hydrolysis of the glycosidic bond, releasing the aglycone and glucose. By breaking down these substrates, β-glucosidase plays a critical role in various biological processes, including the metabolism of glycosides and the release of flavor compounds in wines.
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| ln Vitro |
In vitro, β-Glucosidase activity is typically measured using chromogenic or fluorogenic substrates, such as 4-nitrophenyl-β-D-glucopyranoside. The enzyme hydrolyzes the substrate, releasing 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically at 400 nm. The enzyme is also used in biochemical assays to study the inhibition of glycosidases. β-Glucosidase activity can be quantified using colorimetric microplate assay kits with a detection range of 10 µmol/L to 1000 µmol/L.
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| ln Vivo |
In vivo, β-Glucosidase plays a critical role in the metabolism of glycosides in various organisms. In plants, it is involved in the activation of defense compounds and the release of flavor precursors. In animals, it is involved in the digestion of carbohydrates and the metabolism of xenobiotics. The enzyme's activity is also important in the production of wine, where it releases flavor compounds from glycosylated precursors.
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| Enzyme Assay |
The in vitro enzyme assay for β-Glucosidase typically involves incubating the enzyme with a chromogenic substrate, such as 4-nitrophenyl-β-D-glucopyranoside, in a suitable buffer system. The enzyme hydrolyzes the substrate, releasing 4-nitrophenol, which produces a yellow color. The absorbance is measured at 400 nm, and the activity is calculated based on the rate of 4-nitrophenol release. The assay can be performed in a microplate format for high-throughput screening of inhibitors. The enzyme is typically diluted in sodium acetate buffer (100 mM), pH 4.0, containing 1 mg/mL BSA.
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| Cell Assay |
Cellular assays for β-Glucosidase typically involve the use of cell lines that express the enzyme. Cells are lysed, and the lysate is incubated with a chromogenic substrate. The production of the chromophore is measured to quantify enzyme activity. Alternatively, live cells can be used to measure the hydrolysis of fluorogenic substrates that are taken up by the cells. The enzyme's activity and its modulation by inhibitors or other compounds can be assessed.
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| Animal Protocol |
In vivo animal studies for β-Glucosidase typically involve the use of rodent models to study the enzyme's role in metabolism and disease. The enzyme's activity can be measured in tissue homogenates. The effects of β-glucosidase inhibitors on carbohydrate digestion and glucose absorption can be assessed. The compound's ability to modulate glycoside metabolism and its potential therapeutic applications can be evaluated.
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| ADME/Pharmacokinetics |
As an enzyme, β-Glucosidase does not have conventional pharmacokinetic properties like absorption, distribution, metabolism, and excretion (ADME). However, the enzyme's stability and activity can be affected by temperature, pH, and the presence of inhibitors or cofactors. The enzyme is typically stored at 4°C in an ammonium sulphate suspension. For research purposes, the enzyme is supplied as a lyophilized powder or as a solution in buffer.
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| Toxicity/Toxicokinetics |
β-Glucosidase is generally considered non-toxic as a research reagent. The enzyme itself is a naturally occurring protein and does not present significant toxicity concerns in laboratory settings. Standard laboratory safety practices should be followed when handling the enzyme preparation, including the use of personal protective equipment. The enzyme is typically handled as a routine laboratory reagent.
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| References | |
| Additional Infomation |
β-Glucosidase (EC 3.2.1.21) is a glycosidase enzyme that catalyzes the hydrolysis of glycosidic bonds in various substrates, including beta-D-glucosides. It is used in research, biochemical enzyme assays, and analytical testing applications. β-Glucosidase is used to reveal and enhance the flavors of wines through the hydrolysis of glycosylated aroma precursors. The enzyme is typically supplied as an ammonium sulphate suspension and should be stored at 4°C.
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| Molecular Weight |
0
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| Exact Mass |
283.069
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| CAS # |
9001-22-3
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| Appearance |
White to light yellow solid powder
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| LogP |
0.017
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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