| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| Other Sizes |
| Targets |
1-Naphthyl phosphate potassium salt targets phosphatases in a non-specific manner, inhibiting acid phosphatases, alkaline phosphatases, and protein phosphatases. The compound acts as a competitive inhibitor by binding to the active site of phosphatase enzymes, thereby preventing the dephosphorylation of natural substrates. Its broad-spectrum activity makes it a useful tool for studying phosphatase-dependent processes, although its lack of specificity limits its use in targeted mechanistic studies.
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| ln Vitro |
While PF3 does not react to phosphatase inhibitor treatment, the splice-correcting effect achieved with NickFect1 (NF1) and NickFect2 (NF2) diminishes by approximately 50% in the presence of 1-Naphthyl phosphate potassium salt (N7000). When 1-Naphthyl Phosphate Potassium Salt is present, the shape and packing density of ON complexes with NF1/2 are less regular[1].
In vitro, 1-Naphthyl phosphate potassium salt demonstrates potent inhibition of non-specific phosphatase activity. The compound is used as a substrate in phosphatase activity assays, where its dephosphorylation can be monitored by the release of naphthol, which can be detected colorimetrically or fluorometrically. As an inhibitor, it can suppress phosphatase activity in cell lysates and biochemical reactions. The compound's activity is concentration-dependent, with typical effective concentrations ranging from 1-100 µM. |
| ln Vivo |
In vivo activity data for 1-Naphthyl phosphate potassium salt are limited, as the compound is primarily used as an in vitro research reagent. Due to its non-specific phosphatase inhibition, the compound would not be suitable for in vivo therapeutic applications. Its potential utility in animal models has not been explored, and the compound is not intended for use in living organisms. Any in vivo effects would likely be non-specific and potentially toxic.
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| Enzyme Assay |
The in vitro enzyme assay for 1-Naphthyl phosphate potassium salt typically involves measuring phosphatase activity using the compound as either a substrate or an inhibitor. For substrate assays, the compound is incubated with phosphatase enzyme in appropriate buffer, and the release of naphthol is measured at 405 nm or by fluorescence. For inhibition assays, varying concentrations of the compound (1-1000 µM) are pre-incubated with the enzyme before adding a standard substrate, and residual activity is measured.
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| Cell Assay |
For in vitro cell-based assays, cells are cultured and lysed, and the cell lysate is incubated with 1-Naphthyl phosphate potassium salt. Phosphatase activity is measured by monitoring the dephosphorylation of the compound or by using a standard phosphatase substrate. The compound can be used to inhibit cellular phosphatase activity to study the effects on signaling pathways. Typical concentrations range from 10-500 µM depending on the cell type and assay conditions.
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| Animal Protocol |
In vivo animal studies for 1-Naphthyl phosphate potassium salt are not commonly performed, as the compound is a research reagent rather than a drug candidate. If used in vivo, the compound would likely be administered via intravenous or intraperitoneal injection, but its pharmacokinetics, efficacy, and safety have not been characterized. The non-specific nature of its phosphatase inhibition would make interpretation of in vivo results challenging.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 1-Naphthyl phosphate potassium salt have not been characterized, as the compound is a research reagent. The compound is soluble in water at 50 mg/mL. It appears as a white to faint yellow powder. Stability in solution may be limited. The compound should be stored under recommended conditions and fresh solutions should be prepared for each experiment. Molecular weight is 300.33 or 262.24 depending on the salt form.
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| Toxicity/Toxicokinetics |
Toxicology data for 1-Naphthyl phosphate potassium salt are limited. The compound has GHS hazard communication with a warning signal word. It should be handled with standard laboratory safety precautions. The compound may cause skin and eye irritation. It is not intended for human use and its safety profile for therapeutic applications has not been evaluated. Appropriate personal protective equipment should be used when handling the compound.
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| References | |
| Additional Infomation |
1-Naphthyl phosphate potassium salt is a non-specific phosphatase inhibitor and substrate widely used in biochemical research. It is a valuable tool for studying phosphatase activity, enzyme kinetics, and signal transduction pathways. The compound's broad-spectrum activity makes it useful for identifying phosphatase-dependent processes, although its lack of specificity limits targeted mechanistic studies. It is intended for research use only and is not approved for therapeutic or diagnostic applications.
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| Molecular Formula |
C10H7K2O4P
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|---|---|
| Molecular Weight |
300.33
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| Exact Mass |
261.98
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| CAS # |
100929-85-9
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| PubChem CID |
91871704
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
2.749
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
17
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| Complexity |
251
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[KH].OP(OC1=CC=CC2=CC=CC=C12)(O)=O
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| InChi Key |
BLJABTDVVCCOPM-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C10H9O4P.2K/c11-15(12,13)14-10-7-3-5-8-4-1-2-6-9(8)10;;/h1-7H,(H2,11,12,13);;/q;2*+1/p-2
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| Chemical Name |
dipotassium;naphthalen-1-yl phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3297 mL | 16.6484 mL | 33.2967 mL | |
| 5 mM | 0.6659 mL | 3.3297 mL | 6.6593 mL | |
| 10 mM | 0.3330 mL | 1.6648 mL | 3.3297 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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