| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
|
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| Other Sizes |
| Targets |
MAOS does not target a biological receptor or enzyme. It is a chemical reagent used in analytical assays. In the presence of H₂O₂ and a peroxidase enzyme (such as horseradish peroxidase), MAOS reacts to form a colored product that can be measured spectrophotometrically.
|
|---|---|
| ln Vitro |
In vitro, MAOS is used in enzymatic assays to measure the production of H₂O₂. The chromogenic reaction is strongly dependent on the pH of the reaction medium. MAOS is used in a wide range of pH conditions.
|
| ln Vivo |
MAOS is not used in vivo. Its application is limited to in vitro analytical chemistry and biochemical assays.
|
| Enzyme Assay |
Cell-free assays for MAOS involve its use as a chromogenic substrate in peroxidase-coupled reactions. The assay typically contains the sample (containing H₂O₂), peroxidase, and MAOS. The reaction produces a colored product, and the absorbance is measured at a specific wavelength.
|
| Cell Assay |
In vitro cellular assays for MAOS are not typical, as the compound is used in cell-free systems to measure H₂O₂ in biological samples, such as cell culture supernatants. It is not used to treat cells.
|
| Animal Protocol |
In vivo animal studies are not applicable for MAOS, as it is a chemical reagent used in laboratory assays, not a drug.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for MAOS, as it is a chemical reagent and not a therapeutic agent.
|
| Toxicity/Toxicokinetics |
MAOS is not associated with toxicity, as it is a chemical reagent used in laboratory assays. Standard laboratory safety practices apply when handling the compound.
|
| References | |
| Additional Infomation |
MAOS is a research reagent with no clinical applications or regulatory approvals. It is widely used as a chromogenic probe for the determination of H₂O₂ in enzymatic assays and clinical chemistry. Its high water solubility and stability make it a preferred choice for Trinder's reaction-based assays.
|
| Molecular Formula |
C13H20NNAO4S
|
|---|---|
| Molecular Weight |
309.36
|
| Exact Mass |
309.101
|
| CAS # |
82692-97-5
|
| PubChem CID |
53393748
|
| Appearance |
White to off-white solid powder
|
| LogP |
2.116
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
20
|
| Complexity |
361
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCN(CC(CS(=O)(=O)[O-])O)C1=CC(=CC(=C1)C)C.[Na+]
|
| InChi Key |
HLXGRHNZZSMNRX-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C13H21NO4S.Na/c1-4-14(8-13(15)9-19(16,17)18)12-6-10(2)5-11(3)7-12;/h5-7,13,15H,4,8-9H2,1-3H3,(H,16,17,18);/q;+1/p-1
|
| Chemical Name |
sodium;3-(N-ethyl-3,5-dimethylanilino)-2-hydroxypropane-1-sulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 125 mg/mL (404.06 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2325 mL | 16.1624 mL | 32.3248 mL | |
| 5 mM | 0.6465 mL | 3.2325 mL | 6.4650 mL | |
| 10 mM | 0.3232 mL | 1.6162 mL | 3.2325 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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