| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
Not applicable; this is a stable isotope-labeled analytical standard. Formamide-15N is not designed to target biological receptors but rather to serve as a tracer or internal standard. The unlabeled parent compound, formamide, is a DNA denaturant that significantly reduces DNA stability in buffer solutions. However, the ¹⁵N-labeled version is primarily used for analytical method development and metabolite tracing studies.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Formamide-15N is used as an internal standard in mass spectrometry and NMR spectroscopy to quantify unlabeled formamide in biological or environmental samples. It does not exert direct pharmacological activity in cell-free systems. Its value lies in having nearly identical chemical properties to the natural compound while providing a distinct mass shift (M+1) that allows for precise quantification during analytical measurements. |
| ln Vivo |
In vivo, Formamide-15N is not used as a therapeutic agent. It is incorporated into stable isotope tracing studies to follow metabolic pathways. For example, it can be administered to animal models to track nitrogen flux through one-carbon metabolism or nucleotide biosynthesis pathways. Its safety profile is expected to be similar to unlabeled formamide, which can be toxic at high doses, but labeling itself does not alter inherent toxicity.
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| Enzyme Assay |
For NMR analysis: Dissolve Formamide-15N in a suitable deuterated solvent (e.g., DMSO-d6) to a concentration of 1-10 mM. Acquire ¹H-¹⁵N HMBC (Heteronuclear Multiple Bond Correlation) or ¹⁵N-¹H HSQC (Heteronuclear Single Quantum Coherence) spectra to exploit the ¹⁵N label. For GC-MS or LC-MS: Add a known amount of Formamide-15N (e.g., 10-100 ng/mL) to samples as an internal standard. Quantify by comparing the peak area ratio (analyte/internal standard) against a calibration curve.
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| Cell Assay |
Formamide-15N is not typically used in live cell assays due to potential toxicity of formamide. However, for analytical validation, cell lysates or culture media can be spiked with the labeled compound to serve as an internal standard for quantifying endogenous formamide or formamide metabolites. Process samples by protein precipitation with acetonitrile or methanol, followed by centrifugation and LC-MS/MS analysis. Use this method to correct for matrix effects in biological samples.
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| Animal Protocol |
In animal pharmacokinetic or metabolic studies, Formamide-15N can be administered (e.g., oral gavage or intravenous injection) to track its distribution and metabolism. Blood, urine, and tissue samples are collected at predetermined time points. Samples are processed (e.g., protein precipitation, centrifugation) and analyzed by LC-MS or GC-MS. The labeled compound serves as the tracer; the unlabeled analyte is quantified using the labeled form as an internal standard. Doses vary based on study design.
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| ADME/Pharmacokinetics |
Formamide-15N has a molecular formula CH3NO (with ¹⁵N) and a molecular weight of approximately 46.04 g/mol. It is a colorless to light yellow liquid at room temperature. Purity: ≥99% by GC. Isotopic enrichment: ≥98 atom% ¹⁵N. Store at 2-8degC or -20degC in a sealed container, protected from light. It is stable under recommended storage conditions.
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| Toxicity/Toxicokinetics |
Formamide is classified as a hazardous substance. It is harmful if swallowed, and may cause reproductive harm. The ¹⁵N-labeled variant shares these properties. Always handle with appropriate personal protective equipment (gloves, lab coat, safety glasses) in a well-ventilated fume hood. Avoid contact with skin, eyes, and clothing. Wash hands thoroughly after handling. In case of accidental exposure, seek medical advice. Consult the Safety Data Sheet (SDS) for detailed information.
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| References | |
| Additional Infomation |
Formamide-15N is a versatile research tool in chemistry and biology. It is the simplest monocarboxylic acid amide, derived from formic acid. Beyond analytical applications, it is used as a plasticizer for thermoplastic starch (TPS) and as a solvent for many ionic compounds. The ¹⁵N labeling makes it invaluable for ¹⁵N NMR studies and isotopic tracing in nitrogen metabolism research. It is also used to prepare other labeled compounds.
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| Molecular Formula |
CH3NO
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|---|---|
| Molecular Weight |
45.04062
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| Exact Mass |
46.018
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| CAS # |
1449-77-0
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| Related CAS # |
Formamide;75-12-7
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| PubChem CID |
11029754
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.159 g/mL at 25ºC
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| Boiling Point |
210ºC(lit.)
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| Melting Point |
2-3ºC(lit.)
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| Flash Point |
310 °F
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| Index of Refraction |
n20/D 1.447(lit.)
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| LogP |
-0.8
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
3
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| Complexity |
12.3
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(=O)[15NH2]
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| InChi Key |
ZHNUHDYFZUAESO-VQEHIDDOSA-N
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| InChi Code |
InChI=1S/CH3NO/c2-1-3/h1H,(H2,2,3)/i2+1
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| Chemical Name |
(15N)azanylformaldehyde
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 22.2025 mL | 111.0124 mL | 222.0249 mL | |
| 5 mM | 4.4405 mL | 22.2025 mL | 44.4050 mL | |
| 10 mM | 2.2202 mL | 11.1012 mL | 22.2025 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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