| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
As a stable isotope-labeled tracer, L-Alanine-15N does not exert a pharmacological effect by binding to a biological target. It is used to trace nitrogen metabolism. When administered to cells or organisms, the ¹⁵N label is incorporated into proteins and other nitrogen-containing metabolites, allowing researchers to study nitrogen flux, amino acid turnover, and protein synthesis.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, L-Alanine-15N is used as a tracer in cell culture experiments to study amino acid metabolism. Cells are cultured in media containing the labeled amino acid, and the incorporation of ¹⁵N into proteins and other metabolites is measured using mass spectrometry. This approach allows for the quantification of protein synthesis rates and nitrogen metabolism. |
| ln Vivo |
In vivo, L-Alanine-15N is administered to animals to study whole-body nitrogen metabolism. The tracer can be given orally or intravenously, and the ¹⁵N label is tracked in blood, tissues, and urine to measure amino acid turnover and protein synthesis. This approach is widely used in nutritional and metabolic research.
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| Enzyme Assay |
Cell-free assays for L-Alanine-15N are not typical, as the compound functions as a metabolic tracer in living systems. However, it can be used in in vitro assays to study the kinetics of enzymes involved in alanine metabolism, such as alanine aminotransferase, by tracking the transfer of the ¹⁵N label to reaction products.
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| Cell Assay |
In vitro cellular experiments involve culturing cells in media containing L-Alanine-15N. After a defined incubation period, cells are harvested, and proteins and metabolites are extracted. The ¹⁵N enrichment in specific metabolites is then analyzed by mass spectrometry to determine the rates of protein synthesis and amino acid turnover.
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| Animal Protocol |
In vivo animal studies involve administering L-Alanine-15N to rodents via oral gavage or intravenous injection. Blood and tissue samples are collected at various time points to measure ¹⁵N enrichment in proteins and metabolites. This approach is used to study protein metabolism in various physiological and pathological conditions.
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| ADME/Pharmacokinetics |
The pharmacokinetics of L-Alanine-15N are identical to those of natural L-alanine, as the isotopic substitution does not alter the molecule's chemical properties. It is rapidly absorbed from the gastrointestinal tract, distributed throughout the body, and incorporated into proteins or metabolized via the TCA cycle.
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| Toxicity/Toxicokinetics |
L-Alanine-15N is a stable, non-radioactive isotope that is considered safe for use in research. No significant toxicity is associated with the compound, as it is chemically identical to natural L-alanine. Standard laboratory safety practices apply when handling the compound.
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| References | |
| Additional Infomation |
L-Alanine-15N is a research tool with no clinical applications or regulatory approvals. It is primarily used as a tracer for quantitation during the drug development process and in metabolic research. The compound is valuable for studying nitrogen metabolism in medical, environmental, and industrial research.
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| Molecular Formula |
C3H715NO2
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|---|---|
| Molecular Weight |
90.09
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| Exact Mass |
90.044
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| CAS # |
25713-23-9
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| Related CAS # |
L-Alanine;56-41-7
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| PubChem CID |
11815285
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| Appearance |
White to off-white solid powder
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| Melting Point |
314.5ºC (dec.)(lit.)
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| LogP |
0.118
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
6
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| Complexity |
61.8
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C[C@@H](C(=O)O)[15NH2]
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| InChi Key |
QNAYBMKLOCPYGJ-GZPBOPPUSA-N
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| InChi Code |
InChI=1S/C3H7NO2/c1-2(4)3(5)6/h2H,4H2,1H3,(H,5,6)/t2-/m0/s1/i4+1
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| Chemical Name |
(2S)-2-(15N)azanylpropanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 11.1000 mL | 55.5001 mL | 111.0001 mL | |
| 5 mM | 2.2200 mL | 11.1000 mL | 22.2000 mL | |
| 10 mM | 1.1100 mL | 5.5500 mL | 11.1000 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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