At 1.2 M of L-Alanine, the viability of 201B7 cells and ehiPSCs decreases to 7.5±1.3% and 3.7±0.7%, respectively, as the concentration of L-Alanine increases. The viability of hFBs and hSkMCs, however, does not appear to have decreased. Although iCMs' vitality marginally declines as L-Alanine concentration rises, it is still considerably greater (p<0.01) than that of undifferentiated iPSCs, 201B7 cells, and ehiPSCs at 1.2 M concentration of L-Alanine (49.4±6.9%). Even after two or four hours of treatment, the vitality of 201B7 cells, hiPSCs, and ehiPSCs sharply declines. However, the viability of hFBs exhibits a slight decline at 24 hours of treatment, failing to decrease at 1, 2, and 4 hours. Following a 2-hour treatment with 1.2 M L-Alanine, the vitality of 201B7 cells in suspension culture drops to 11.8±6.0%, while that of hFBs is 72.9±14.2%[1].

[1]. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine. Sci Rep. 2018 Aug 20;8(1):12427.

L-alanine is the L-enantiomer of alanine. It has a role as an EC 4.3.1.15 (diaminopropionate ammonia-lyase) inhibitor and a fundamental metabolite. It is a pyruvate family amino acid, a proteinogenic amino acid, a L-alpha-amino acid and an alanine. It is a conjugate base of a L-alaninium. It is a conjugate acid of a L-alaninate. It is an enantiomer of a D-alanine. It is a tautomer of a L-alanine zwitterion.
Alanine is a non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and the central nervous system.
L-Alanine is a metabolite found in or produced by Escherichia coli (strain K12, MG1655).
Alanine is an Amino Acid.
Alanine has been reported in Euphorbia prostrata, Angelica gigas, and other organisms with data available.
Alanine is a small non-essential amino acid in humans, Alanine is one of the most widely used for protein construction and is involved in the metabolism of tryptophan and vitamin pyridoxine. Alanine is an important source of energy for muscles and central nervous system, strengthens the immune system, helps in the metabolism of sugars and organic acids, and displays a cholesterol-reducing effect in animals. (NCI04)
Alanine is a nonessential amino acid made in the body from the conversion of the carbohydrate pyruvate or the breakdown of DNA and the dipeptides carnosine and anserine. It is highly concentrated in muscle and is one of the most important amino acids released by muscle, functioning as a major energy source. Plasma alanine is often decreased when the BCAA (Branched Chain Amino Acids) are deficient. This finding may relate to muscle metabolism. Alanine is highly concentrated in meat products and other high-protein foods like wheat germ and cottage cheese. Alanine is an important participant as well as regulator in glucose metabolism. Alanine levels parallel blood sugar levels in both diabetes and hypoglycemia, and alanine reduces both severe hypoglycemia and the ketosis of diabetes. It is an important amino acid for lymphocyte reproduction and immunity. Alanine therapy has helped dissolve kidney stones in experimental animals. Normal alanine metabolism, like that of other amino acids, is highly dependent upon enzymes that contain vitamin B6. Alanine, like GABA, taurine and glycine, is an inhibitory neurotransmitter in the brain. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and the central nervous system.
A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.
See also: Glatiramer Acetate (monomer of); Glatiramer (monomer of); Amlisimod (monomer of) ... View More ...

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Drug Indication
Used for protein synthesis.

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Mechanism of Action
L-Alanine is a non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and the central nervous system. BCAAs are used as a source of energy for muscle cells. During prolonged exercise, BCAAs are released from skeletal muscles and their carbon backbones are used as fuel, while their nitrogen portion is used to form another amino acid, Alanine. Alanine is then converted to Glucose by the liver. This form of energy production is called the Alanine-Glucose cycle, and it plays a major role in maintaining the body's blood sugar balance.

C3H7NO2

89.09

89.047

56-41-7

25191-17-7

5950

White to off-white solid powder

1.2±0.1 g/cm3

212.9±23.0 °C at 760 mmHg

314.5 °C (dec.)(lit.)

82.6±22.6 °C

0.1±0.9 mmHg at 25°C

1.460

-0.68

2

3

1

6

61.8

1

C[C@@H](C(=O)O)N

QNAYBMKLOCPYGJ-REOHCLBHSA-N

InChI=1S/C3H7NO2/c1-2(4)3(5)6/h2H,4H2,1H3,(H,5,6)/t2-/m0/s1

(2S)-2-aminopropanoic acid

Alanine AlaninumL-(+)-Alanine Alanina NSC-206315 NSC206315NSC 206315

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

H2O : ~150 mg/mL (~1683.69 mM)
DMSO :< 1 mg/mL

Solubility in Formulation 1: 50 mg/mL (561.23 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.

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 (Please use freshly prepared in vivo formulations for optimal results.)